Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells

Cao, Zhenguo; Chen, Liang

doi:10.3892/etm.2017.5053

Cited by 17 publications

(12 citation statements)

References 34 publications

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“…The preliminarily screened literature was read according to the criteria of oral bioavailability (OB) ≥30% and drug-likeness (DL) ≥0. 18 component of BHD were retrieved. The targets and the effective components of BHD were then inputted into the Perl plug-ins.…”

Section: Effective Components and The Selection Of Targets Of Bhdmentioning

confidence: 99%

The mechanism of Bushen Huoxue decoction in treating intervertebral disc degeneration based on network pharmacology

et al. 2021

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Background: This study aimed to explore the mechanism of Bushen Huoxue decoction (BHD) in treating intervertebral disc degeneration using the network pharmacology method. Methods: Using of oral bioavailability >30% and drug-likeness >0.18 as the screening standards, the effective components and targets of BHD were retrieved from the TCMSP database and the BATMAN-TCM database. The disease targets of intervertebral disc degeneration were retrieved from the GeneCards database. The Wayne map of the interaction targets of the effective components of BHD and intervertebral disc degeneration were drawn using R software. The protein-protein interaction (PPI) network of common targets was constructed using STRING software. The network map of the interaction targets of the effective components of BHD-intervertebral disc degeneration was drawn using Cytoscape3.7.2 software. The GO and KEGG enrichment analysis of the common targets of BHD and intervertebral disc degeneration was performed using R software and the related plug-ins to screen the potential pathways and analyze its mechanism. Results: This study screened 164 effective components of BHD, 131 interaction targets, 626 targets for degenerative disc disease, and 31 common interaction targets. IL6, VEGFA, CASP3, EGFR, ESR1, and MAPK8 appeared more frequently. These were mainly enriched in the AGE-RAGE, TNF, PI3K Akt, and MAPK signaling pathways. Conclusions: BHD mainly intervenes in intervertebral disc degeneration through IL6, VEGFA, CASP3, EGFR, ESR1, and MAPK8. The mechanism of the intervention of BHD on intervertebral disc degeneration may be related to AGE-RAGE, TNF, PI3K Akt, MAPK, and other signal pathways.

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Section: Effective Components and The Selection Of Targets Of Bhdmentioning

confidence: 99%

The mechanism of Bushen Huoxue decoction in treating intervertebral disc degeneration based on network pharmacology

et al. 2021

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“…Novel research has shown that miR-27a promotes ERK phosphorylation by downregulation of the ERK inhibitor sprouty2 (50). It has also been proven that miR-27a regulates the p38/mitogen-activated protein kinase (MAPK) signaling pathway (51). Furthermore, miR-27a directly silences the FBXW7 gene by binding to its 3′ untranslated region (UTR), thereby reducing the expression of FBXW7 in cancer cell.…”

Section: Roles Of Mir-27a In Regulating Signaling Pathwaysmentioning

confidence: 99%

MicroRNA-27a (miR-27a) in Solid Tumors: A Review Based on Mechanisms and Clinical Observations

Zhang

Cao

et al. 2019

Front. Oncol.

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MicroRNAs (miRNAs) are a family of highly conserved, non-coding single-stranded RNAs transcribed as ~70 nucleotide precursors to an 18–22 nucleotide product (1). miRNAs can silence their homologous target genes at the post-transcriptional level, and these genes have been revealed to play an important role in tumorigenesis, invasion and metastasis (2). MicroRNA-27a (miR-27a), transcripted by miR-27a gene, has proved to implicate with many kinds of solid tumors, showing potential as a useful biomarker or drug target for clinical application. However, even though miR-27a has been reported in many cancers, the mechanism and signal pathways of miR-27 in oncogenesis, invasion, and metastasis are still obscure. Moreover, recent studies show that miR-27a pays an important role in epithelial-mesenchymal-transition, regulating tumor immune response, and chemoresistance. In this review, we summarize the current literature, demonstrate the established link between miR-27a and tumorigenesis, and focus on recently identified mechanisms. The review also aims to demonstrate the potential of miR-27a as a diagnostic and/or prognostic biomarker in solid tumors and to discuss the possibilities of targeted therapy and drug design.

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“…The current study tested the hypothesis that MetS modulates in MetS-MSCs the content of miRNA related to senescence. For this purpose, senescence biomarkers were evaluated and MSC miRNA expression characterized using high-throughput RNA sequencing analysis, as well as selective inhibition of miR-27b, a MAPK regulator 14 , which was found to be elevated in MetS-MSCs. Importantly, we characterize the miRNA spectrum that may be involved in regulation of MSC senescence in MetS.…”

Section: Introductionmentioning

confidence: 99%

Micro-RNAS Regulate Metabolic Syndrome-induced Senescence in Porcine Adipose Tissue-derived Mesenchymal Stem Cells through the P16/MAPK Pathway

et al. 2018

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Mesenchymal stem cells (MSCs) constitute an important repair system, but may be impaired by exposure to cardiovascular risk factors. Consequently, adipose tissue-derived MSCs from pigs with the metabolic syndrome (MetS) show decreased vitality. A growing number of microRNAs (miRNAs) are recognized as key modulators of senescence, but their role in regulating senescence in MSC in MetS is unclear. We tested the hypothesis that MetS upregulates in MSC expression of miRNAs that can serve as post-transcriptional regulators of senescence-associated (SA) genes. MSCs were collected from swine abdominal adipose tissue after 16 weeks of Lean or Obese diet (n = 6 each). Next-generation miRNA sequencing (miRNA-seq) was performed to identify miRNAs up-or down-regulated in MetS-MSCs compared with Lean-MSCs. Functional pathways of SA genes targeted by miRNAs were analyzed using gene ontology. MSC senescence was evaluated by p16 and p21 immunoreactivity, H2AX protein expression, and SA-β-Galactosidase activity. In addition, gene expression of p16, p21, MAPK3 (ERK1) and MAPK14, and MSC migration were studied after inhibition of SA-miR-27b. Senescence biomarkers were significantly elevated in MetS-MSCs. We found seven upregulated miRNAs, including miR-27b, and three downregulated miRNAs in MetS-MSCs, which regulate 35 SA genes, particularly MAPK signaling. Inhibition of miR-27b in cultured MSCs downregulated p16 and MARP3 genes, and increased MSC migration. MetS modulates MSC expression of SA-miRNAs that may regulate their senescence, and the p16 pathway seems to play an important role in MetS-induced MSC senescence.

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Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells

Cited by 17 publications

References 34 publications

The mechanism of Bushen Huoxue decoction in treating intervertebral disc degeneration based on network pharmacology

The mechanism of Bushen Huoxue decoction in treating intervertebral disc degeneration based on network pharmacology

MicroRNA-27a (miR-27a) in Solid Tumors: A Review Based on Mechanisms and Clinical Observations

Micro-RNAS Regulate Metabolic Syndrome-induced Senescence in Porcine Adipose Tissue-derived Mesenchymal Stem Cells through the P16/MAPK Pathway

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