Inhibition of murine GVHD by PEN110 treatment

Abstract

PEN110 treatment prevents the development of GVHD and alloantibody production following WBC transfusion in a murine model system, supporting the continued development of PEN110 treatment of cellular blood components as an alternative to gamma irradiation for the prevention of GVHD.

Novel processes for inactivation of leukocytes to prevent transfusion-associated graft-versus-host disease

“…In contrast, animals transfused with donor T cells treated with amotosalen and UVA survived without developing TA-GVHD and were engrafted with donor hematopoietic cells. 47 The homozygous parent to F 1 hybrid transfusion model also has been used by Fast et al 48 to evaluate the ability of the Inactine compound PEN 110 to prevent TA-GVHD. Treated cells did not cause TA-GVHD.…”

Prevention ofYersinia enterocolitica,Pseudomonas fluorescens, andPseudomonas putidaoutgrowth in deliberately inoculated blood by a novel pathogen‐reduction process

“…The INACTINE process for preparation of pathogen‐reduced RBCs combines chemical inactivation of pathogenic microorganisms 11,12 with RBC purification 13,14 to improve the transfusion safety of RBCs 15‐20 . The process utilizes PEN110, a small, positively charged electrophilic molecule that is chemically related to binary ethyleneimine.…”

Genotypic regulation of alloantibody production in response to UVB‐irradiated allogeneic donor cells

“…The blood was centrifuged at 800 × g for 10 minutes and the plasma removed and stored at −20°C. The presence of alloantibody in the plasma was measured by flow cytometric (FACscan, Becton Dickinson, San Jose, CA) quantitation of the level of alloantibody binding to donor thymocytes as previously described 9 . Thymocytes were used because the paucity of Fc receptor bearing cells and B cells in this population contributed to a low background when secondary anti‐mouse immunoglobulin reagents were used.…”