Inhibition of NETosis via PAD4 Could Alleviate the Inflammation of Giant Cell Myocarditis

Zhan, Hongbing; Hua, Xiumeng; Mo, Xiuxue; Chen, Yuan; Chen, Xiao; Xu, Zhenyu; Tao, Mengtao; Hu, Gang; Song, Jiangping

doi:10.2139/ssrn.4250467

Cited by 1 publication

(1 citation statement)

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“…Single-cell RNA-Seq data preprocessing and clustering with DESC Single-cell RNA-Seq data preprocessing and clustering with DESC was performed according to previously method [32]. Briefly, single-cell RNA-seq data were pre-processed and qualitied with Seurat (version 4) [33].…”

Section: Single-cell Rna-seq Data Analysesmentioning

confidence: 99%

Muscularis macrophages controlled by NLRP3 maintain the homeostasis of excitatory neurons

Gao,

Shi,

Wei

et al. 2024

Int. J. Biol. Sci.

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Peristaltic movements in gut are essential to propel ingested materials through the gastrointestinal tract. Intestinal resident macrophages play an important role in this physiological function through protecting enteric neurons. However, it is incompletely clear how individuals maintain the homeostasis of gut motility. Here we found that NLRP3 is a critical factor in controlling loss of muscularis resident macrophages (MMs), and demonstrate that MMs are involved in the homeostasis of excitatory neurons such as choline acetyltransferase (ChAT) + and vesicular glutamate transporter 2 (VGLUT2) + but not inhibitory neuronal nitric oxide synthase (nNOS) + neurons. NLRP3 knockout (KO) mice had enhanced gut motility and increased neurons, especially excitatory ChAT + and VGLUT2 + neurons. Single cell analyses showed that there had increased resident macrophages, especially MMs in NLRP3 KO mice. The MM proportion in the resident macrophages was markedly higher than those in wild-type (WT) or caspase 1/11 KO mice. Deletion of the MMs and transplantation of the NLRP3 KO bone marrow cells showed that survival of the gut excitatory ChAT + and VGLUT2 + neurons was dependent on the MMs. Gut microbiota metabolites β-hydroxybutyrate (BHB) could promote gut motility through protecting MMs from pyroptosis. Thus, our data suggest that MMs regulated by NLRP3 maintain the homeostasis of excitatory neurons.

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Section: Single-cell Rna-seq Data Analysesmentioning

confidence: 99%