Inhibition of neutrophil infiltration by a malleable protein matrix of lactic acid bacteria-fermented whey proteins in vivo

Beaulieu, Josée; Girard, Denis; Dupont, Claude; Lemieux, Pierre

doi:10.1007/s00011-009-7100-y

Cited by 12 publications

(11 citation statements)

References 44 publications

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“…In line with our findings, Sprong et al ( 1 ) reported that cheese whey protein protected rats against mild dextran sulphate Na-induced colitis by inhibiting the expressions of inflammatory cytokines. Whey product has demonstrated a number of anti-inflammatory effects including decreased cytokine release in rodent models after exposure to LPS ( 42 ) . It is possible that the protective effects of WPC supplementation on intestinal integrity were closely related to reducing the expressions of intestinal pro-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-β1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge

et al. 2016

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Whey protein concentrate (WPC) has been reported to have protective effects on the intestinal barrier. However, the molecular mechanisms involved are not fully elucidated. Transforming growth factor-β1 (TGF-β1) is an important component in the WPC, but whether TGF-β1 plays a role in these processes is not clear. The aim of this study was to investigate the protective effects of WPC on the intestinal epithelial barrier as well as whether TGF-β1 is involved in these protection processes in a piglet model after lipopolysaccharide (LPS) challenge. In total, eighteen weanling pigs were randomly allocated to one of the following three treatment groups: (1) non-challenged control and control diet; (2) LPS-challenged control and control diet; (3) LPS + 5 %WPC diet. After 19 d of feeding with control or 5 %WPC diets, pigs were injected with LPS or saline. At 4 h after injection, pigs were killed to harvest jejunal samples. The results showed that WPC improved (P < 0·05) intestinal morphology, as indicated by greater villus height and villus height:crypt depth ratio, and intestinal barrier function, which was reflected by increased transepithelial electrical resistance and decreased mucosal-to-serosal paracellular flux of dextran (4 kDa), compared with the LPS group. Moreover, WPC prevented the LPS-induced decrease (P < 0·05) in claudin-1, occludin and zonula occludens-1 expressions in the jejunal mucosae. WPC also attenuated intestinal inflammation, indicated by decreased (P < 0·05) mRNA expressions of TNF-α, IL-6, IL-8 and IL-1β. Supplementation with WPC also increased (P < 0·05) TGF-β1 protein, phosphorylated-Smad2 expression and Smad4 and Smad7 mRNA expressions and decreased (P < 0·05) the ratios of the phosphorylated to total c-jun N-terminal kinase (JNK) and p38 (phospho-JNK:JNK and p-p38:p38), whereas it increased (P < 0·05) the ratio of extracellular signal-regulated kinase (ERK) (phospho-ERK:ERK). Collectively, these results suggest that dietary inclusion of WPC attenuates the LPS-induced intestinal injury by improving mucosal barrier function, alleviating intestinal inflammation and influencing TGF-β1 canonical Smad and mitogen-activated protein kinase signalling pathways.

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Section: Discussionmentioning

confidence: 99%

Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-β1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge

et al. 2016

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“…Moreover, it was also shown that MPM, upon oral gavage in diet-induced obese mice, may act as a potential peroxisome proliferator-activated receptor gamma (PPARγ) agonist (Lapointe et al, 2009). This could occur indirectly via the action of MPM on insulin or via reduction of specific cytokines and inflammation markers, considering the antiinflammatory effect of MPM and its ability to inhibit cytokine and chemokine production (i.e., IL-1β, IL-6, and tumor necrosis factor-α) in murine immune disease models (Beaulieu et al, 2007b(Beaulieu et al, , 2009a.…”

Section: Discussionmentioning

confidence: 99%

The whey fermentation product malleable protein matrix decreases triglyceride concentrations in subjects with hypercholesterolemia: A randomized placebo-controlled trial

Berthold

Schulte

Lapointe³

et al. 2011

Journal of Dairy Science

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Malleable protein matrix (MPM) is a unique whey-derived ingredient obtained through a fermentation process using proprietary lactic acid bacteria strains from the Lactobacillus kefiranofaciens species. Because evidence from animal models suggests that MPM decreases serum lipid concentrations, the purpose of the present trial was to assess the hypothesis that MPM exerts lipid-lowering effects in humans. A total of 161 subjects (50% male; age 54.5 ± 9.8 yr, body mass index 26.3 ± 3.6 kg/m(2)) with hypercholesterolemia with baseline low-density lipoprotein cholesterol (LDL-C) levels of 181 ± 30 mg/dL and normal triglyceride (TG) levels (131 ± 55 mg/dL) were randomized to receive MPM (2 × 15 g/d) or matching placebo. A 6-wk run-in phase was followed by a double-blind 12-wk treatment phase after randomization. The data were analyzed on an intention-to-treat basis. The primary outcome measure was the percentage change of LDL-C. The secondary outcome measures were changes in TG and high-density lipoprotein cholesterol concentrations as well as changes in other cardiovascular risk factors. After 12 wk of treatment, the relative TG decrease from baseline reached 9.8%, whereas LDL-C was slightly decreased (by 1.5%) following MPM treatment compared with placebo in the intention-to-treat cohort. The treatment effect on TG reduction was much higher in the subset of subjects having TG levels at baseline of 150 mg/dL or above (n=42), reaching 20.0% compared with placebo. High-density lipoprotein cholesterol concentrations, blood pressure, and fasting blood glucose remained unchanged, whereas a positive treatment effect was seen on hemoglobin A(1c). The MPM product was tolerated well without severe adverse events. In conclusion, MPM has significant TG-lowering properties in subjects with combined hypercholesterolemia and higher TG levels. Its effects on LDL-C concentrations and glucose metabolism deserve further investigation.

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“…Clinical studies have found that [40]. In addition, many studies reported that probiotics, and homogenates of lactic acid bacteria are effective for preventing cancer [29], improving absorption of lactose and milk proteins [15], decreasing cholesterol [42], treating rheumatoid arthritis [5], and enhancing intestinal regulation [20]. Thus, in terms of the functionality of lactic acid bacteria, this study confirmed that antimicrobial and anti-allergic activity was increased by culturing the lactic acid bacteria in a medium containing the supernatant of lactic acid bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…casei had strain-specific therapeutic effects on allergy [25,40]. Various studies also showed that probiotics, killed lactic acid bacteria, and lactic acid bacteria lysates induced anti-cancer effect [29], improved absorption of lactose and milk proteins [15], lower serum cholesterol levels [42], treatment for rheumatoid arthritis [5], and intestinal regulation [20].…”

Section: Introductionmentioning

confidence: 99%

Double-culture Method Enhances the in Vitro Inhibition of Atopy-inducing Factors by Lactococcus lactis

Jo¹,

Kang²,

Kim³

2015

Journal of Life Science

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We analyzed whether lactic acid bacteria could control the expression of IL-4 and IL-13 in activated mast cells and whether these bacteria could inhibit the activity of transcription factors such as GATA-1, GATA-2, NF-AT1, NF-AT2, and NF-κB p65. We previously described a technique for identification of lactic acid bacteria with anti-atopy functionality by confirming increased expression of CD4+/CD25+/foxp3+ in T cells. We also confirmed that a double-culture method increased the antibacterial activity of these lactic acid bacteria against Staphylococcus aureus (S. aureus). In the present study, we characterized the effect of lactic acid bacteria cultured by this double-culture method on inhibition of allergic inflammatory reactions of RBL-2H3 mast cells, a cellular model of atopic dermatitis. The strongest anti-allergic effects of the lactic acid bacteria were seen in the following order: Lactococcus lactis broth cultured with medium containing Lactobacillus plantarum culture supernatant > Lc. lactis > Lc. lactis broth cultured with medium containing Lb. plantarum culture supernatant > Lb. plantarum. Thus, Lc. lactis cultured in medium containing Lb. plantarum culture supernatant had the strongest inhibitory effect on the differentiation of mast cells during allergic reactions, which may be mediated through the selective regulation of expression of relevant genes.

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Inhibition of neutrophil infiltration by a malleable protein matrix of lactic acid bacteria-fermented whey proteins in vivo

Cited by 12 publications

References 44 publications

Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-β1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge

Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-β1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge

The whey fermentation product malleable protein matrix decreases triglyceride concentrations in subjects with hypercholesterolemia: A randomized placebo-controlled trial

Double-culture Method Enhances the in Vitro Inhibition of Atopy-inducing Factors by Lactococcus lactis

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