Inhibition of Neutrophil Pholasin ChemiluminescencebyCleome arabicaLeaf Extract

Abstract

The antioxidative activity of Cleome arabica L. leaf extract was studied toward superoxide anion radical generating systems. The extract at 10 mg=ml has no scavenger effect on superoxide anion radicals generated by the xanthine-xanthine oxidase system, as determined by either the reduction of cytochrome c or pholasin luminescence. Also, this extract, up to 50 mg=ml, did not affect the uric acid production by xanthine oxidase. However, the extract showed a great inhibition activity, in a dose-dependent manner, of neutrophil pholasin luminescence stimulated by formyl-methionyl-leucylphenylalanine (fMLP). Indeed, the inhibition of pholasin luminescence was 80.22 AE 2.9%, 90.22 AE 4.0%, and 101.0 AE 1.0% in presence of the extract at concentrations of 1, 2, and 10 mg=ml, respectively. Thereby, it is likely that Cleome arabica leaf extract showed an inhibitory effect on enzyme(s) involved in signaling pathways of fMLP-stimulated neutrophils. Effectively, the extract exerted a significant inhibitory effect (p < 0.05), in a dose-dependent manner, on elastase release by fMLP/ cytochalasin B-stimulated neutrophils.

Cleome arabicaleaf extract has anticancer properties in human cancer cells

“…Less information is available on botanical extracts that contain a mixture of flavonoids. Cleome arabica is well known in traditional medicine: its botanical extract with high antioxidant capacities (Djeridane et al, 2010;Selloum et al, 2004) could reduce inflammatory conditions both in vivo and in vitro (Bouriche et al, 2003. These effects have been related to the flavonoid content of CALE.…”

“…It has been reported that the polyphenolic extract of C. arabica leaves (CALE) could reduce inflammatory conditions either in vivo by reducing rat paw edema, in vitro by modulating the activity of lipoxygenase and calcium-stimulated generation of the leukotriene B4 and prostaglandin E2 in human neutrophils or also by inhibiting their chemotaxis (Bouriche et al, 2003(Bouriche et al, , 2004. These CALE actions seem to be related to the high content of polyphenolic compounds (Bouriche et al, 2003;Djeridane et al, 2010;Ismail et al, 2005).…”

“…These CALE actions seem to be related to the high content of polyphenolic compounds (Bouriche et al, 2003;Djeridane et al, 2010;Ismail et al, 2005). Indeed, C. arabica leaf extract contains flavonoids such as glycosylated quercetin, kaempferol and isorhamnetin (Ismail et al, 2005) that also increase the antioxidant capacities of this extract (Djeridane et al, 2010;Selloum et al, 2004).…”