Inhibition of NO
 3
 −
 and NO
 2
 −
 Reduction by Microbial Fe(III) Reduction: Evidence of a Reaction between NO
 2
 −
 and Cell Surface-Bound Fe
 2+

Coby, Aaron J.; Picardal, Flynn W.

doi:10.1128/aem.71.9.5267-5274.2005

Cited by 81 publications

(58 citation statements)

References 48 publications

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“…We believe that the encrustations formed with Fe 2ϩ in batch reactors are artifacts of the millimolar-level Fe 2ϩ and NO 3 Ϫ concentrations typically used in Ϫ and Fe 2ϩ sorbed onto cell surfaces (7,10). In the present study, NO 2 Ϫ was measured in cultures containing Fe 2ϩ but not in medium O which contained only acetate as the electron donor.…”

Section: Discussionmentioning

confidence: 61%

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Chakraborty

Roden

Schieber

et al. 2011

Appl Environ Microbiol

146

140

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Microbial nitrate-dependent, Fe(II) oxidation (NDFO) is a ubiquitous biogeochemical process in anoxic sediments. Since most microorganisms that can oxidize Fe(II) with nitrate require an additional organic substrate for growth or sustained Fe(II) oxidation, the energetic benefits of NDFO are unclear. The process may also be self-limiting in batch cultures due to formation of Fe-oxide cell encrustations. We hypothesized that NDFO provides energetic benefits via a mixotrophic physiology in environments where cells encounter very low substrate concentrations, thereby minimizing cell encrustations. Acidovorax sp. strain 2AN was incubated in anoxic batch reactors in a defined medium containing 5 to 6 mM NO 3 ؊ , 8 to 9 mM Fe 2؉ , and 1.5 mM acetate. Almost Microbially driven, Fe(III)/Fe(II) redox transitions in the environment have a dramatic impact on iron's solubility, mineralogy, sorption characteristics, and overall geochemical properties (21,29,45). Microbially mediated redox transformations of Fe can also affect the biogeochemical cycling of other key nutrients (e.g., C, S, P, and N) (15, 29), trace metals (9, 49), metalloids (12), and the fate of organic pollutants (25) and contaminant metals (8), including those released from industrial and mining areas (30). Anaerobic, microbial Fe(III) reduction has been studied extensively over the last 30 years (31, 45), and much is known about the microbiology and geochemistry of that process.Chemotrophic, anoxic Fe(II) oxidation under circumneutral conditions, on the other hand, was first described only 15 years ago (42), and little is known about the microbiology of the process or its actual significance in the environment. Microbial nitrate-dependent, Fe(II) oxidation (NDFO) has been demonstrated in a variety of sediments, microbial consortia, and pure cultures, including both autotrophic and heterotrophic cultures. With respect to autotrophic growth via NDFO, for example, the mesophilic enrichment culture originally described by Straub et al. (42) has been maintained for over a decade under autotrophic conditions and is capable of near complete oxidation of Fe(II) and reduction of NO 3

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Section: Discussionmentioning

confidence: 61%

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Chakraborty

Roden

Schieber

et al. 2011

Appl Environ Microbiol

146

140

View full text Add to dashboard Cite

show abstract

“…In all cultures, mineral precipitation at the cell surface was probably initially avoided by a protective EPS layer surrounding the cells, which potentially complexed Fe(III) and/or inhibited crystal nucleation and crystal growth because of binding of Fe ions by the organics (55). When nitrite diffuses through this EPS layer, green rust and goethite minerals form on the outside of the EPS layer, catalyzing Fe(II) oxidation and Fe(III) mineral formation (31,46,69). This mechanism would also explain the observations of (i) Fe(III) mineral formation without direct contact with the cells despite the low solubility of Fe(III) (14), (ii) oxidation of Fe(II) by sterile-filtered supernatants (67), and (iii) formation of an Fe(III) mineral coating on Shewanella putrefaciens cells incubated with Fe 2ϩ and NO 2 Ϫ (69).…”

Section: Discussionmentioning

confidence: 99%

Potential Role of Nitrite for Abiotic Fe(II) Oxidation and Cell Encrustation during Nitrate Reduction by Denitrifying Bacteria

Klueglein

Zeitvogel

Stierhof

et al. 2014

Appl Environ Microbiol

183

239

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“…), (ii) extracellular or intracellular mineral phase Fe(II) oxidation coupled to the reduction of NO 3 Ϫ or biogenic NO 2 Ϫ and NO (48) (green rusts have been shown to form during NDFO by Acidovorax sp. strain BoFeN1 [49]), (iii) proton-dependent abiotic Fe(II) reduction of NO 2 Ϫ under the lower-pH conditions that exist in the bacterial periplasm, and (iv) Fe(II) oxidation coupled to the reduction of NO 2 Ϫ and NO catalyzed by periplasmic components such as protein thiols (3, 41, 50) or membrane-bound iron (51,52).…”

Section: Evidence Against An Inducible Fe(ii) Oxidoreductase Inmentioning

confidence: 99%

Fe(II) Oxidation Is an Innate Capability of Nitrate-Reducing Bacteria That Involves Abiotic and Biotic Reactions

Carlson

Clark²,

Blazewicz³

et al. 2013

Journal of Bacteriology

155

160

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Phylogenetically diverse species of bacteria can catalyze the oxidation of ferrous iron [Fe(II)] coupled to nitrate (NO 3؊ ) reduction, often referred to as nitrate-dependent iron oxidation (NDFO). Very little is known about the biochemistry of NDFO, and though growth benefits have been observed, mineral encrustations and nitrite accumulation likely limit growth. Acidovorax ebreus, like other species in the Acidovorax genus, is proficient at catalyzing NDFO. Our results suggest that the induction of specific Fe(II) oxidoreductase proteins is not required for NDFO. No upregulated periplasmic or outer membrane redox-active proteins, like those involved in Fe(II) oxidation by acidophilic iron oxidizers or anaerobic photoferrotrophs, were observed in proteomic experiments. We demonstrate that while "abiotic" extracellular reactions between Fe(II) and biogenic NO 2 ؊ /NO can be involved in NDFO, intracellular reactions between Fe(II) and periplasmic components are essential to initiate extensive NDFO. We present evidence that an organic cosubstrate inhibits NDFO, likely by keeping periplasmic enzymes in their reduced state, stimulating metal efflux pumping, or both, and that growth during NDFO relies on the capacity of a nitrate-reducing bacterium to overcome the toxicity of Fe(II) and reactive nitrogen species. On the basis of our data and evidence in the literature, we postulate that all respiratory nitrate-reducing bacteria are innately capable of catalyzing NDFO. Our findings have implications for a mechanistic understanding of NDFO, the biogeochemical controls on anaerobic Fe(II) oxidation, and the production of NO 2 ؊ , NO, and N 2 O in the environment.

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Inhibition of NO ₃ ⁻ and NO ₂ ⁻ Reduction by Microbial Fe(III) Reduction: Evidence of a Reaction between NO ₂ ⁻ and Cell Surface-Bound Fe ²⁺

Cited by 81 publications

References 48 publications

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Potential Role of Nitrite for Abiotic Fe(II) Oxidation and Cell Encrustation during Nitrate Reduction by Denitrifying Bacteria

Fe(II) Oxidation Is an Innate Capability of Nitrate-Reducing Bacteria That Involves Abiotic and Biotic Reactions

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Inhibition of NO 3 − and NO 2 − Reduction by Microbial Fe(III) Reduction: Evidence of a Reaction between NO 2 − and Cell Surface-Bound Fe 2+

Cited by 81 publications

References 48 publications

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Enhanced Growth of Acidovorax sp. Strain 2AN during Nitrate-Dependent Fe(II) Oxidation in Batch and Continuous-Flow Systems

Potential Role of Nitrite for Abiotic Fe(II) Oxidation and Cell Encrustation during Nitrate Reduction by Denitrifying Bacteria

Fe(II) Oxidation Is an Innate Capability of Nitrate-Reducing Bacteria That Involves Abiotic and Biotic Reactions

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Inhibition of NO ₃ ⁻ and NO ₂ ⁻ Reduction by Microbial Fe(III) Reduction: Evidence of a Reaction between NO ₂ ⁻ and Cell Surface-Bound Fe ²⁺