Inhibition of nuclear translocation of notch intracellular domain (NICD) by diosgenin prevented atherosclerotic disease progression

Monocytes treated with ciprofloxacin and oxyLDL express myristate, priming atherosclerosis

“…Cell morphological changes were observed in the ciprofloxacin‐treated cells starting at 48 hours. The morphology of these cells was continuously observed up to 14 days, which showed gradual morphological changes from monocyte to plaque‐like cells on day 12 and foam cells, which are an intermediate stage in atherosclerosis, observed at day 8, which was confirmed with Oil Red O staining …”

“…Lipoproteins have been classified by their electrophoretic mobility. [6,14,15] In this study, the electrophoretic mobility of oxidized lipoproteins (LDL) showed greater electrophoretic mobility than the native lipoproteins. [16] Major classes of bactericidal antibiotics have been demonstrated to induce oxidative damage in bacteria, leading to the generation of ROS.…”

“…The concentration used in this study was less than the clinically relevant dose. Lipoproteins have been classified by their electrophoretic mobility . In this study, the electrophoretic mobility of oxidized lipoproteins (LDL) showed greater electrophoretic mobility than the native lipoproteins …”

“…The morphology of these cells was continuously observed up to 14 days, which showed gradual morphological changes from monocyte to plaque-like cells on day 12 and foam cells, which are an intermediate stage in atherosclerosis, observed at day 8, which was confirmed with Oil Red O staining. [6,15] Further flow cytometry analysis with CD64 and CD23 was performed to confirm monocyte-macrophage polarization, which showed an increase in the proinflammatory macrophage phenotype of up to 18% in oxyLDL-induced ciprofloxacin-treated cells when compared with the oxyLDL-induced cells. The cytokine and chemokine profile of macrophages can define their activation state.…”

The Use of Nutraceuticals to Counteract Atherosclerosis: The Role of the Notch Pathway

“…Norisoboldine, an alkaloid compound isolated from Radix Linderae, suppresses synovial angiogenesis, thanks to its inhibition of VEGF-induced endothelial cell migration via a cAMP-PKA-NF-κB/Notch-1 signaling pathway [301]. Both in rat aortic subintimal macrophages and in in vitro-differentiated macrophage cell diosgenin, a phytosteroid sapogenin extracted from the tubers of Dioscorea wild yam suppresses the nuclear translocation of NICD [302]. Resveratrol can attenuate neointimal VSMC hyperplasia in rats, following balloon injury, through a mechanism that involves inhibition of the Notch signaling [303].…”