Inhibition of Nucleostemin Upregulates CDX2 Expression in HT29 Cells in Response to Bile Acid Exposure: Implications in the Pathogenesis of Barrett’s Esophagus

Abstract

These findings suggest that the inhibition of nucleostemin expression in "esophageal stem cells" in response to bile acid exposure may be involved in the pathogenesis of BE through upregulating CDX2 expression.

Systematic review: the role of bile acids in the pathogenesis of gastro-oesophageal reflux disease and related neoplasia

“…A review of the references from five review articles 8, 94–97 yielded two additional original articles that were reviewed and met inclusion criteria 98, 99 . One article was excluded after initial manuscript preparation when it was recognised that the cell line used (HT‐29) was colonic (not oesophageal) adenocarcinoma 25 . Thus, a total of 83 original articles met the inclusion criteria and were included in the final review.…”

“…98,99 One article was excluded after initial manuscript preparation when it was recognised that the cell line used (HT-29) was colonic (not oesophageal) adenocarcinoma. 25 Thus, a total of 83 original articles met the inclusion criteria and were included in the final review.…”

“…After initial title and abstract review, 123 complete articles were considered potentially relevant and retrieved for full review. Of these, 82 original articles met the inclusion criteria 12–93 . Forty‐one articles were excluded for the following reasons: 3 – not original articles; 1 – duplicate reference; 7 – animal (nonhuman) study; 6 – use of a cell line initially thought to be oesophageal that later was determined non‐oesophageal (i.e., SEG‐1); 21 – no oesophageal bile acid exposure or measurement (11 – duodenogastric reflux; 5 – bilirubin measurement; 5 – other); and 3 – no relevant outcomes provided.…”