Inhibition of osteoclast differentiation and bone resorption by rotenone, through down-regulation of RANKL-induced c-Fos and NFATc1 expression

Kwak, Han Bok; Lee, Byeong Ki; Oh, Jae‐Min; Yeon, Jeong-Tae; Choi, Sik‐Won; Cho, Hae Joong; Lee, Myeung Su; Kim, Jeong-Joong; Bae, Ji-Myung; Kim, Seong Hwan; Kim, Hun Soo

doi:10.1016/j.bone.2009.10.042

Cited by 82 publications

(72 citation statements)

References 29 publications

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“…To further elucidate the underlying mechanism involved in miR-218-triggered inhibition on osteoclastogenesis and bone resorption, the p38MAPK signaling was investigated. In accordance with our hypothesis, miR-218 elevation strikingly inhibited the phosphorylation of p38, as well as the down-regulation of its downstream targets of c-Fos and NFATC1, both crucial regulators for osteoclast differentiation and bone resorption [30]. Importantly, pretreatment with P79350 notable abated the inhibitory effect of miR-218 on osteoclast differentiation, migration, actin ring formation and bone-resorbing activity.…”

Section: Discussionsupporting

confidence: 87%

miR-218 is involved in the negative regulation of osteoclastogenesis and bone resorption by partial suppression of p38MAPK-c-Fos-NFATc1 signaling: Potential role for osteopenic diseases

Xia

Yan

et al. 2015

Experimental Cell Research

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Section: Discussionsupporting

confidence: 87%

miR-218 is involved in the negative regulation of osteoclastogenesis and bone resorption by partial suppression of p38MAPK-c-Fos-NFATc1 signaling: Potential role for osteopenic diseases

Xia

Yan

et al. 2015

Experimental Cell Research

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“…Previous studies showed that imatinib mesylate and baicalein decrease bone resorption by interfering with osteoclast survival (Hajj et al, 2006;Kim et al, 2008). Also, capsazepine and rotenone inhibit bone resorption by downregulating the activity of osteoclasts (Idris et al, 2010;Kwak et al, 2010). In the present study, econazole inhibited bone resorption activity of OLCs, but had no significant effect on OLC survival.…”

Section: Discussioncontrasting

confidence: 43%

Inhibition of bone resorption by econazole in rat osteoclast-like cells through suppressing TRPV5

et al. 2011

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Osteoclasts are primary bone resorption cells and intervention in osteoclast activation is considered an effective therapeutic approach to treatment of bone diseases involving osteoclasts. TRPV5 was detected in osteoclasts and it has been thought to take part in the transportation of the degraded calcium in the resorption lacuna, which is essential for bone resorption. The aim of the present study was to examine the effects of a modulator of calcium dynamics, econazole, on the expression of TRPV5 and bone resorption activity in rat osteoclast-like cells (OLCs). OLCs were obtained by co-culturing rat bone marrow cells with osteoblasts and then culturing with different concentrations of econazole (0.01, 0.1, 1.0, 10.0 μmol/L). Cell counting and staining protocols were used to determine whether econazole influenced the survival of OLCs. Expression of TRPV5 in response to econazole treatment was assessed by western blotting. Bone resorption activity of OLCs was determined by measuring the resorption area of dentin slices with a microscope and a digital image analysis system. Additionally, Ca(2+) inside OLCs was tested. We found that econazole inhibited expression of TRPV5 in a dose dependent manner while it had no influence on the survival of OLCs and it therefore inhibited bone resorption activity in rat OLCs. Ca(2+) inside OLCs increased, suggesting a limited compensatory mechanism to make up for inhibition of TRPV5 effects.

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“…Furthermore, Stewartia koreana extracts and berberine from Coptidis rhizoma have also been reported (Han et al 2007;Park et al 2007;Hu et al 2008;Kim et al 2009;Kwak et al 2010).…”

Section: Introductionmentioning

confidence: 94%

Isolation and identification of RANKL-induced osteoclast differentiation inhibitor from Pleurotus citrinopileatus

et al. 2013

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Keywords: b-Glucan golden oyster mushroom Medicinal mushroom Osteoporosis Physiological functionality a b s t r a c tThe purpose of this study was to investigate the effects of water extracts from edible mushroom, Pleurotus citrinopileatus fruiting body on RANKL-induced osteoclast differentiation. The osteoclast differentiation inhibitor from water extracts of P. citrinopileatus was purified by ultrafiltration, size exclusion column chromatography, ethanol precipitation, and treatment with various enzymes. The water extracts from P. citrinopileatus showed significant osteoclast differentiation inhibitory activity, and its 50 kDa above fraction from ultrafiltration showed the highest osteoclast differentiation inhibitory activity. Fractions 2 and 3 from size exclusion column chromatography clearly inhibited RANKL-induced osteoclast differentiation. When these fractions were treated by ethanol precipitation, the precipitates showed high osteoclast differentiation inhibitory activity. Finally, we obtained the precipitates as the purified osteoclast differentiation inhibitor, and it was identified as b-glucan. However, further studies are required to be used as candidate of anti-osteoporotic agent.Crown

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Inhibition of osteoclast differentiation and bone resorption by rotenone, through down-regulation of RANKL-induced c-Fos and NFATc1 expression

Cited by 82 publications

References 29 publications

miR-218 is involved in the negative regulation of osteoclastogenesis and bone resorption by partial suppression of p38MAPK-c-Fos-NFATc1 signaling: Potential role for osteopenic diseases

miR-218 is involved in the negative regulation of osteoclastogenesis and bone resorption by partial suppression of p38MAPK-c-Fos-NFATc1 signaling: Potential role for osteopenic diseases

Inhibition of bone resorption by econazole in rat osteoclast-like cells through suppressing TRPV5

Isolation and identification of RANKL-induced osteoclast differentiation inhibitor from Pleurotus citrinopileatus

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