Inhibition of oxidative stress and gastric emptying as additional mechanisms of antidiabetic activity of Newbouldia laevis

Mbagwu, Ikechukwu Sonne; Akah, Peter A.; Ajaghaku, Amara Anwuchaepe; Ugwu, Obiora Celestine; Ajaghaku, Daniel Lotanna

doi:10.1016/j.phyplu.2021.100023

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…"High levels of alkaline phosphatase (ALP) and alanine aminotransferase (ALT), which are typical of oxidative stress conditions, were differentially ameliorated after treatment with the ethanol extracts of N. laevis leaves and stem in a dose-dependent manner. Our previous study on the leaves extract also reveals strong antioxidant potentials both in vitro and in vivo" [12].…”

Section: Discussionmentioning

confidence: 95%

“…We have previously conducted extensive studies on the ethanol leaf extract and fractions of N. laevis which include establishment of the median antidiabetic effective dose [12]; establishment of improved effect on glucose and fat homeostasis in a type-2 diabesity mice model [13]; demonstration that inhibition of oxidative stress and gastric emptying are part of it mechanisms of antidiabetic activity [12] and the isolation of two new caffeic acid glycosides (Newboulasides A and B) with α-amylase inhibitory activity [14]. Since diabetes requires prolonged time for management and treatment which might be associated with accumulated drug toxicity, this study evaluated the sub-chronic toxicity of the ethanol leaf extract of N. laevis in animal model of toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Median Lethal Dose and Target Organ Toxicity of Oral Sub-chronic Administration of Newbouldia laevis in Mice and Rat Model of Toxicity

Mbagwu,

Orji,

Ani

et al. 2023

JOCAMR

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The acute and sub-chronic toxicities of the leaf extract of Newbouldia laevis, an ethnomedicinal herb use in the management of diabetes mellitus was investigated. For the acute toxicity study, 10 – 5000 mg/kg of the extract were administered orally to mice and obvious signs of toxic symptoms and mortality monitored for 24 h post extract administration. In the sub-chronic study, 302 and 604 mg/kg of the extract were orally administered daily for 90 days. Body weight changes as well as haematological and biochemical parameters were determined periodically. Qualitative phytochemistry was also conducted. Presence of flavonoids, saponins, tannins, reducing sugar, steroids, terpenoids, alkaloids and glycosides phytocompounds in the extract were detected. The oral LD50 was estimated to be above 5,000 mg/kg in mice. Ninety days oral administration of ethanol extract of N. laevis produced a significant (P<0.05) reduction in body weight at 604 mg/kg on the 31st day and at both 302 and 604 mg/kg on the 61st and 91st days compared to 5% Tween 20 vehicle control group. For liver function enzymes, the extract at both doses (302 and 604 mg/kg) produced significant (P<0.05) reduction in serum ALT enzyme activity at the 91st day with non-significant reduction in other liver function enzymes compared to vehicle control group. Non-significant changes were also recorded for haematological and kidney function markers. The results from this study provide evidence for safety profile of the ethanol leaves extract of N. laevis thus supportive of its validity in the use for treatment of chronic diseases like diabetes.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Assessment of Median Lethal Dose and Target Organ Toxicity of Oral Sub-chronic Administration of Newbouldia laevis in Mice and Rat Model of Toxicity

Mbagwu,

Orji,

Ani

et al. 2023

JOCAMR

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“…The WHO advocates for the use of natural products in the management of ailments (Süntar, 2020). Several plants have been shown to have antidiabetic properties, among them the trio of Leptadenia hastata, Newbouldia laevis and Albizzia chevalieri which were previously reported to have antidiabetic action in type 1 diabetic rodent models (Saidu et al, 2010;Bello et al, 2011;Kabir et al, 2021;Mbagwu et al, 2021). Thus, we aimed to determine whether the methanolic extracts of these plants would have an antidiabetic and thrombolytic effect in a rat model of high fat diet and dexamethasone induced T2DM.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and Thrombolytic Activities of Some Selected Medicinal Plants in High Fat Diet and Dexamethasone-Induced Type 2 Diabetic Rats

Ibrahim,

Shinkafi,

Ishaka

et al. 2024

J. Afr. Assoc. Physiol. Sci

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Background: Type-2 diabetes (T2D) is a hypercoagulable and hypofibrinolytic condition that predisposes to cardiovascular and thrombotic complications. We screened three medicinal plants (Albizzia chevalieri, Newbouldia laevis and Leptadenia hastate) for their antidiabetic and thrombolytic activities. Methods: T2D was induced with high fat diet and dexamethasone. Following induction, rats were grouped into 6 (n=8 rats); control, untreated, treated (500mg/kg body weight (BW) metformin only) or treated (300mg/kg BW leaf extracts). The rats were treated for two weeks and euthanized. About 2ml of the collected blood was used for thrombolytic activity assay while the rest was processed, and the recovered serum utilised for biochemical and hormonal assays. Results: Rats treated with extracts had significantly (p<0.05) lowered concentrations of serum glucose, TG, LDL-cholesterol, VLDL- cholesterol and increased HDL-cholesterol compared to untreated rats. Extracts also lowered (p<0.05) the serum insulin concentration on day 14 compared to untreated rats. Albizzia chevalieri, Newbouldia laevis and Leptadenia hastata showed 48.90%, 39.20% and 37.69% clot lysis activities respectively which was significantly higher (p<0.001) than distilled water, while streptokinase produced a substantial clot lysis of 93.70%. Conclusions: The leaf extracts of Albizzia chevalieri, Newbouldia laevis and Leptadenia hastata have antidiabetic and thrombolytic activities in type-2 diabetic rats and thus, can be potentially used as combined antidiabetic and thrombolytic agents with in vivo effects in type-2 diabetic patients.

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In vivo antidiabetic and antioxidant activities of chloroform fraction of Nelsonia canescens Leaf in Alloxan-induced Diabetic Rats

Daniel¹,

Gara²,

Ibrahim³

et al. 2022

Pharmacological Research - Modern Chinese Medicine

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Inhibition of oxidative stress and gastric emptying as additional mechanisms of antidiabetic activity of Newbouldia laevis

Cited by 3 publications

References 32 publications

Assessment of Median Lethal Dose and Target Organ Toxicity of Oral Sub-chronic Administration of Newbouldia laevis in Mice and Rat Model of Toxicity

Assessment of Median Lethal Dose and Target Organ Toxicity of Oral Sub-chronic Administration of Newbouldia laevis in Mice and Rat Model of Toxicity

Antidiabetic and Thrombolytic Activities of Some Selected Medicinal Plants in High Fat Diet and Dexamethasone-Induced Type 2 Diabetic Rats

In vivo antidiabetic and antioxidant activities of chloroform fraction of Nelsonia canescens Leaf in Alloxan-induced Diabetic Rats

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