Inhibition of ozone-induced SP-A oxidation by plant polyphenols

Stagos, Dimitrios; Umstead, Todd M.; Phelps, David S.; Skaltsounis, Léandros; Haroutounian, Serko; Floros, Joanna; Kouretas, Demetrios

doi:10.1080/10715760601064714

Cited by 9 publications

(8 citation statements)

References 59 publications

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“…We speculate that this increase occurs because of the lack of SP-A, an important host defense protein that plays an antioxidant or oxidant scavenger role in the alveolus. This is based on several converging lines of evidence including: 1) the attribution of an antioxidant role to SP-A [101][102][103]; 2) the demonstration that SP-A is highly susceptible to oxidative modification by carbonylation) [53] and to ozone-induced oxidation of methionine residues [34], and that its function is disrupted by these oxidative modifications) [9,[31][32][33][34]; and 3) the description of other systems in which proteins serve as "sacrificial antioxidants" [104][105][106][107][108][109].…”

Section: Discussionmentioning

confidence: 99%

“…Many of the changes in WTO 3 mice are likely due to oxidative stress resulting from acute ozone exposure. We have demonstrated that SP-A is highly susceptible to oxidative modification and that its modification significantly compromises its function)) [31][32][33]53,[115][116][117]. In WT mice SP-A is an abundant BAL protein and several lines of evidence have linked it to redox regulation and led investigators to propose an antioxidant function for SP-A.…”

Section: Comparison Of Wt and Ko Micementioning

confidence: 97%

“…These include serving as an opsonin for the recognition of some pathogens [23,24], regulating the production of cell surface antigens and inflammatory mediator expression by some immune cells [25,26], participating in the development of dendritic cells [27], regulating reactive oxidant production [28,29], and others [30]. However, a series of studies from our laboratory has shown that several of these functions are compromised when SP-A is oxidized) [9,[31][32][33][34]. A number of studies have explored the function of SP-A in vivo by subjecting SP-A-/-(SP-A knockout; KO) mice to various infectious or environmental challenges.…”

Section: Introductionmentioning

confidence: 99%

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The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

et al. 2009

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BackgroundOzone is a major component of air pollution. Exposure to this powerful oxidizing agent can cause or exacerbate many lung conditions, especially those involving innate immunity. Surfactant protein-A (SP-A) plays many roles in innate immunity by participating directly in host defense as it exerts opsonin function, or indirectly via its ability to regulate alveolar macrophages and other innate immune cells. The mechanism(s) responsible for ozone-induced pathophysiology, while likely related to oxidative stress, are not well understood.MethodsWe employed 2-dimensional difference gel electrophoresis (2D-DIGE), a discovery proteomics approach, coupled with MALDI-ToF/ToF to compare the bronchoalveolar lavage (BAL) proteomes in wild type (WT) and SP-A knockout (KO) mice and to assess the impact of ozone or filtered air on the expression of BAL proteins. Using the PANTHER database and the published literature most identified proteins were placed into three functional groups.ResultsWe identified 66 proteins and focused our analysis on these proteins. Many of them fell into three categories: defense and immunity; redox regulation; and protein metabolism, modification and chaperones. In response to the oxidative stress of acute ozone exposure (2 ppm; 3 hours) there were many significant changes in levels of expression of proteins in these groups. Most of the proteins in the redox group were decreased, the proteins involved in protein metabolism increased, and roughly equal numbers of increases and decreases were seen in the defense and immunity group. Responses between WT and KO mice were similar in many respects. However, the percent change was consistently greater in the KO mice and there were more changes that achieved statistical significance in the KO mice, with levels of expression in filtered air-exposed KO mice being closer to ozone-exposed WT mice than to filtered air-exposed WT mice.ConclusionWe postulate that SP-A plays a role in reactive oxidant scavenging in WT mice and that its absence in the KO mice in the presence or absence of ozone exposure results in more pronounced, and presumably chronic, oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Comparison Of Wt and Ko Micementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

et al. 2009

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“…High levels of ROS react with cellular components including DNA, proteins, and lipids leading to cellular and tissue damage [278]. Resveratrol and other dietary stilbenes reduce oxidative stress by acting either as a direct scavenger of ROS [279] or as an inhibitor of NADPH oxidase expression and xanthine oxidase activity [280]. Resveratrol has low toxicity [281].…”

Section: Approachesmentioning

confidence: 99%

Cancer prevention and therapy through the modulation of the tumor microenvironment

Casey

Amedei

Aquilano

et al. 2015

Seminars in Cancer Biology

312

204

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Cancer arises in the context of an in vivo tumor microenvironment. This microenvironment is both a cause and consequence of tumorigenesis. Tumor and host cells co-evolve dynamically through indirect and direct cellular interactions, eliciting multiscale effects on many biological programs, including cellular proliferation, growth, and metabolism, as well as angiogenesis and hypoxia and innate and adapative immunity. Here we highlight specific biological processes that could be exploited as targets for the prevention and therapy of cancer. Specifically, we describe how inhibition of targets such as cholesterol synthesis and metabolites, reactive oxygen species and hypoxia, macrophage activation and conversion, indoleamine 2, 3-dioxygenase regulation of dendritic cells, vascular endothelial growth factor regulation of angiogenesis, fibrosis inhibition, endoglin, and Janus kinase signaling emerge as examples of important potential nexuses in the regulation of tumorigenesis and the tumor microenvironment that can be targeted. We have also identified therapeutic agents as approaches, in particular natural products such as berberine, resveratrol, onionin A, epigallocatechin gallate, genistein, curcumin, naringenin, desoxyrhapontigenin, piperine, and zerumbone, that may warrant further investigation to target the tumor microenvironment for the treatment and/or prevention of cancer.

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“…However, the precise mechanisms by which SP-A protects the lungs from oxidant-induced cellular injury remain unclear. Interestingly, we now recognize that in addition to the protective effects of SP-A against oxidant-induced cellular damage, the function of SP-A itself can be impaired by oxidation 91–93 . Inhalation of ozone impairs the functional interaction between SP-A and either lung macrophages or alveolar epithelia 94–95 .…”

Section: Genes Of Innate Immunity and Ambient Ozonementioning

confidence: 99%

Ambient ozone and pulmonary innate immunity

et al. 2010

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Ambient ozone is a criteria air pollutant that impacts both human morbidity and mortality. The effect of ozone inhalation includes both toxicity to lung tissue and alteration of the host immunologic response. The innate immune system facilitates immediate recognition of both foreign pathogens and tissue damage. Emerging evidence supports that ozone can modify the host innate immune response and that this response to inhaled ozone is dependent on genes of innate immunity. Improved understanding of the complex interaction between environmental ozone and host innate immunity will provide fundamental insight into the pathogenesis of inflammatory airways disease. We review the current evidence supporting that environmental ozone inhalation: (1) modifies cell types required for intact innate immunity, (2) is partially dependent on genes of innate immunity, (3) primes pulmonary innate immune responses to LPS, and (4) contributes to innate-adaptive immune system cross-talk.

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Inhibition of ozone-induced SP-A oxidation by plant polyphenols

Cited by 9 publications

References 59 publications

The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

Cancer prevention and therapy through the modulation of the tumor microenvironment

Ambient ozone and pulmonary innate immunity

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