Inhibition of P2X7 Purinergic Receptor Ameliorates Fibromyalgia Syndrome by Suppressing NLRP3 Pathway

D’Amico, Ramona; Fusco, Roberta; Siracusa, Rosalba; Impellizzeri, Daniela; Peritore, Alessio Filippo; Gugliandolo, Enrico; Interdonato, Livia; Sforza, Andrea Maria; Crupi, Rosalia; Cuzzocrea, Salvatore; Genovese, Tiziana; Cordaro, Marika; Paola, Rosanna Di

doi:10.3390/ijms22126471

Cited by 39 publications

(30 citation statements)

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“…A fibromyalgia-like model was induced by reserpine injection (1 mg/kg, subcutaneous) for three consecutive days [26]. Reserpine (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in distilled water with 0.5% acetic acid.…”

Section: Induction Of Fibromyalgiamentioning

confidence: 99%

“…The dose and route of HD treatment were chosen based on previous studies [26][27][28]. At the end of the experiment, the L4-L6 region of the spinal cord from each rat was collected for all analyses.…”

Section: Experimental Groupsmentioning

confidence: 99%

“…The supernatant of the homogenate of spinal cord tissue was centrifuged and operated [26,33]. The expression of interleukin (IL)-18, tumor necrosis factor (TNF)-α, and IL-1β were measured using ELISA kits (R&D Systems, Minneapolis, MN, USA) following the manufacturer's instructions.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…The Electronic von Frey test (BIO-EVF4, Bioseb, Vitrolles, France) was used to evaluate mechanical allodynia, as previously explained [26]. All the results are expressed as the force, in grams, at which the rat removed its paw, indicated as the withdrawal threshold.…”

Section: Von Frey Hair Testmentioning

confidence: 99%

“…This test was used to evaluate spinal thermal sensitivity. The duration of the tail withdrawal reflex was recorded as previously described [26]. The cut off time was 10 s to minimize tissue damage to the tail.…”

Section: Tail-flick Warm Water Testmentioning

confidence: 99%

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Wnt/β-Catenin Pathway in Experimental Model of Fibromyalgia: Role of Hidrox®

et al. 2021

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Fibromyalgia (FM) is a chronic condition characterized by persistent widespread pain that negatively affects the quality of life of patients. The WNT/β-catenin signaling pathway seems to be involved in central sensitization and different pain states. The objective of this study was to investigate the beneficial effects of a new compound called Hidrox® (HD), containing 40–50% hydroxytyrosol, in counteracting the pain associated with FM. An FM-like model was induced in rats by subcutaneous injections of reserpine (1 mg/kg) for three consecutive days. Later, HD (10 mg/kg) was administered orally to the animals for seven days. Reserpine injections induced WNT/β-catenin pathway activation, release of pro-inflammatory mediators as well as a significant increase in oxidative stress. Daily treatment with HD was able to modulate the WNT/β-catenin and Nrf2 pathways and consequently attenuate the behavioral deficits and microglia activation induced by reserpine injection. These results indicate that nutritional consumption of HD can be considered as a new therapeutic approach for human FM.

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Section: Induction Of Fibromyalgiamentioning

confidence: 99%

Section: Experimental Groupsmentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Section: Von Frey Hair Testmentioning

confidence: 99%

Section: Tail-flick Warm Water Testmentioning

confidence: 99%

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Wnt/β-Catenin Pathway in Experimental Model of Fibromyalgia: Role of Hidrox®

et al. 2021

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Natural polyphenolic coffee extract administration relieves chronic nociplastic pain in a reserpine‐induced fibromyalgia‐like female mouse model

Toledano‐Martos,

Bagó‐Mas,

Deulofeu

et al. 2024

Brain and Behavior

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IntroductionNociplastic pain involves reflexive and nonreflexive pain responses and it is a core symptom of fibromyalgia (FM). The increasing prevalence of this health condition and the low rates of patients’ quality of life, combined with the lack of suitable pharmacologic treatments, evidence the demand to research new alternatives. Polyphenols may be potential therapeutic candidates as they have been reported to exert pathological pain modulation in preclinical models. In that context, this work was aimed to study the antinociceptive effects of a polyphenolic extract obtained from decaffeinated ground roasted coffee, in the RIM6 FM‐like mouse model.MethodsTo this end, RIM6 adult ICR‐CD1 female mice were administered daily once a week with either 10 or 15 mg/kg of extract, and reflexive pain responses were evaluated for up to 3 weeks. At the end, the depressive‐like behavior was assessed as a nonreflexive pain response, and spinal cord and serum samples were collected for immunohistochemical and toxicological analyses.ResultsThese findings showed that the repeated administration of the coffee polyphenolic extract (CE) modulated reflexive pain responses, depressive‐like behavior, and spinal cord gliosis in a dose‐dependent manner, without signs of systemic toxicity.ConclusionThus, the CE may be a potential pharmacological treatment suitable to relieve nociplastic pain responses characteristic of FM.

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