Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: a potential host for the expression of heterologous proteins

Abstract

BackgroundBacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. The ability of pathogens to integrate within a biofilm is pivotal to their survival. The possibility of biofilm formation in Lactobacillus communities is also important in various industrial and medical settings. Lactobacilli can eliminate the colonization of different pathogenic microorganisms. Alternatively, new opportunities are now arising with the rapidly expanding potential of lactic acid bacteria biofilms as bio-control agents against food-borne pathogens.ResultsA new isolate Lactobacillus plantarum PA21 could form a strong biofilm in pure culture and in combination with several pathogenic and food-spoilage bacteria such as Salmonella enterica, Bacillus cereus, Pseudomonas fluorescens, and Aeromonas hydrophila. Exposure to Lb. plantarum PA21 significantly reduced the number of P. fluorescens,A. hydrophila and B. cereus cells in the biofilm over 2-, 4- and 6-day time periods. However, despite the reduction in S. enterica cells, this pathogen showed greater resistance in the presence of PA21 developed biofilm, either in the planktonic or biofilm phase. Lb. plantarum PA21 was also found to be able to constitutively express GFP when transformed with the expression vector pMG36e which harbors the gfp gene as a reporter demonstrating that the newly isolated strain can be used as host for genetic engineering.ConclusionIn this study, we evaluate the ability of a new Lactobacillus isolate to form strong biofilm, which would provide the inhibitory effect against several spoilage and pathogenic bacteria. This new isolate has the potential to serve as a safe and effective cell factory for recombinant proteins.

Efficacy of Using Probiotics with Antagonistic Activity against Pathogens of Wound Infections: An Integrative Review of Literature

“…On the other hand, experiments on large animals, such as pigs, whose skin has been regarded as the closest surrogate to human skin with regard to similarities in structure and healing, have a disadvantage of extensive costs, handling, and lack of genetic manipulability [131,135]. Certain probiotics have been reported to form robust biofilms in vitro and shown to attach to various host biofilm sites; these include L. casei, L. rhamnosus, L. plantarum, L. reuteri, L. acidophilus, B. bifidum, and B. breve [136][137][138][139][140][141]. Although probiotics form similar biofilm modalities as pathogens, research and evaluation of these biofilms has only occurred in recent years and not necessarily on the skin [43].…”

Bactofection of SW620 cell by Lactococcus lactis M4

“…Due to their certification as GRAS (Generally Recognized As Safe) organisms by the US Food and Drug Administration (FDA), the potential of LAB in several applications have been greatly enhanced. LAB have been reported to be utilized as cell factories for the expression of membrane proteins (Douillard et al, 2011), suppression of spoilage and growth of pathogenic bacteria (Jalilsood et al, 2015), production of biocatalyst (Hugenholtz et al, 2000) and delivery of therapeutic substances (Braat et al, 2006;Rottiers et al, 2009). It was suggested that, replacing the attenuated pathogenic bacteria as a delivery vector for therapeutic vaccine with LAB portrays a much safer alternative for cancer treatments (García-Fruitós, 2012).…”

<i>Lactobacillus</i> sp.—A Threat to Pathogenic Microorganisms and Tumor Cells

“…Studies show that the antimicrobial activity of Lactobacillus was responsible for the expression of heterologous protein. GFP expression was confirmed by the presence of a 27-kDa protein [54]. Another report shows that bacteriocins produced by lactic acid bacteria exert similar characteristics to microsins.…”