Inhibition of phosphate and arsenate uptake in yeast by monoiodoacetate, fluoride, 2,4-dinitrophenol and acetate

Borst-Pauwels, G.W.F.H.; Jager, Sarah de

doi:10.1016/0005-2728(69)90136-4

Cited by 41 publications

(10 citation statements)

References 20 publications

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“…In contrast, cells acclimated to chemostat growth at 0.05 h −1 did not respond to increased orthophosphate availability, despite the abiotic concentration being only on the order of 1 mM. These findings parallel the prior descriptions of the “over(hyper)compensation” effect (Weimberg, 1975) and yeast impermeability to phosphate (Borst‐Pauwels and Jager, 1969). However, the intracellular view of phosphate dynamics provided by NMR adds more detail.…”

Section: Discussionsupporting

confidence: 85%

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NMR-Observed Phosphate Trafficking and Polyphosphate Dynamics in Wild-Type and vph1-1 Mutant Saccharomyces cerevisae in Response to Stresses

1999

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The phosphagenic, osmotic, and metabolic roles of polyphosphate in chemostat-cultivated yeast were investigated with a new NMR cultivator. Wild-type yeast and a vacuolar vph1-1 mutant, which lacks polyphosphate, were subjected to different stimuli. Starved wild-type yeast exclusively directed phosphate to vacuoles despite other competing sinks. After DNP or iodoacetate exposure, which significantly affected cytosolic pH or ATP metabolism, polyphosphate hydrolysis did not occur, which casts doubt on the phosphagen function of vacuolar polyphosphate. It took about 1 h for Mn2+ to traffic to vacuoles, and some evidence was obtained for polyphosphate responding to osmotic challenges. Fast NMR scans show that rapid polyphosphate hydrolysis to small polymers follows alkalinization. The small polymers then degrade to orthophosphate, which coincides with sugar phosphates increasing and subsequent reacidification. In contrast, when vph1-1 mutants were subjected to alkalinization, the absence of a vacuolar source of phosphate slowed reacidification. Based on known yeast physiology and observed sugar phosphate dynamics, polyphosphate degradation may enable rapid glycogen mobilization to glycolysis for considerable acid and ATP production. Overall, maintaining both polyphosphate and carbohydrate reserves may endow yeast with the ability to rapidly manage the extracellular environment.

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Section: Discussionsupporting

confidence: 85%

“…This new peak is attributed to extracellular phosphate. Its location and appearance are consistent with DNP's inhibitory effect on phosphate uptake (Loomis and Lipmann, 1948; Borst‐Pauwels and Jager, 1969). Despite the reduced phosphate influx, polyphosphate is not hydrolyzed.…”

Section: Discussionsupporting

confidence: 63%

NMR-Observed Phosphate Trafficking and Polyphosphate Dynamics in Wild-Type and vph1-1 Mutant Saccharomyces cerevisae in Response to Stresses

1999

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“…Other authors have found that high concentrations of acetate inhibit the uptake of sugars in Aspergillus (15) and that short-chain fatty acids (2, 17) as well as DNP (2,11,13) inhibit the uptake of phosphate by yeast.…”

Section: Fraction Nlmbermentioning

confidence: 96%

Effects of Fatty Acids on Growth and Envelope Proteins of Bacillus subtilis

1972

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Fatty acids of different chain lengths were added to cultures of Bacillus subtilis growing in nutrient sporulation medium, and the effects of these fatty acids on growth, oxygen uptake, adenosine triphosphate (ATP) concentration, and membrane protein composition were examined. All fatty acids inhibited growth, the effect being reduced in the presence of glycolytic compounds and reversed by transfer to medium without fatty acids. The inhibition of growth was correlated with a reduction in both the rate of oxygen consumption and the concentration of ATP per cell. The concentration required to obtain a certain degree of inhibition increased with decreasing molecular weight of the fatty acid. However, the reduced nicotinamide adenine dinucleotide oxidation system of cell envelope preparations (i.e., the electron transport system) was not inhibited. Submaximal growth inhibition was accompanied by the relative increase of a membrane protein band revealed by urea-acetic acid gel electrophoresis. This increase was blocked by actinomycin or chloramphenicol. All of the above changes could also be produced by 2,4-dinitrophenol. The inhibition results are best explained by assuming that the fatty acids reversibly react with the cell membrane or proteins in it; they could either alter the membrane structure or uncouple the electron transport chain from two types of proteins, those used for ATP regeneration and others needed for the transport of certain compounds into the cells.

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“…Kinetic values for accumulation of phosphate, calcium and sulphate ions were, moreover, similar to those already reported for these transport systems in strains of S . cerevisiae grown aerobically (Borst-Pauwels & Jager, 1969;Fuhrmann & Rothstein, 1968;Breton & Surdin-Kerjan, 1977). Values for KT and VmaY for accumulation of L-asparagine and L-glutamine, which have not previously been reported for S .…”

Section: Eflect Of Storage Time On Solute-accumulating Ability Of Orgmentioning

confidence: 99%

Effect of Plasma-membrane Phospholipid Unsaturation on Solute Transport into Saccharomyces cerevisiae NCYC 366

Keenan¹,

Rose²,

Silverman³

1982

Microbiology

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A comparison was made of kinetics of solute accumulation by Saccharomyces cerevisiae NCYC 366 grown anaerobically under conditions that lead to enrichment of the plasma membrane with ergosterol and either oleyl or linoleyl residues. Values for KT and V,,, were identical for accumulation of L-asparagine, L-glutamine, H,PO,, Ca2+ and SO:-, while for accumulation of D-glucose, the value differed slightly but not significantly. Values for KT for accumulation of L-lysine, by both the lowand high-affinity systems, decreased when oleyl residues were replaced by linoleyl residues. Under these conditions, V,,, values for the high-affinity system decreased while that for the low-affinity system increased. An Arrhenius plot for accumulation of lysine by the high-affinity system revealed a discontinuity when membranes were enriched in linoleyl residues. However, no discontinuity was evident on plots of lysine accumulation when membranes were enriched in oleyl residues. Similar plots for accumulation of L-asparagine, which was used as a control, showed that substitution of linoleyl for oleyl residues significantly raised the transition temperature, but had little effect on the activation energy at temperatures below the discontinuity. When palmitoleyl residues were incorporated into the yeast plasma membrane, the KT value for L-lysine accumulation by the high-affinity system was hardly altered, although the V,,, value was lowered, as compared with organisms with membranes enriched in oleyl residues. Replacement of oleyl by palmitoleyl residues lowered both the KT and V,,, values for accumulation of L-asparagine. A modified statistical method is described for calculating confidence limits for transition points on Arrhenius plots.

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Inhibition of phosphate and arsenate uptake in yeast by monoiodoacetate, fluoride, 2,4-dinitrophenol and acetate

Cited by 41 publications

References 20 publications

NMR-Observed Phosphate Trafficking and Polyphosphate Dynamics in Wild-Type and vph1-1 Mutant Saccharomyces cerevisae in Response to Stresses

NMR-Observed Phosphate Trafficking and Polyphosphate Dynamics in Wild-Type and vph1-1 Mutant Saccharomyces cerevisae in Response to Stresses

Effects of Fatty Acids on Growth and Envelope Proteins of Bacillus subtilis

Effect of Plasma-membrane Phospholipid Unsaturation on Solute Transport into Saccharomyces cerevisiae NCYC 366

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