Inhibition of phototrophically growing Chromatium vinosum D by glycine

Effects of glycine on cell growth and pigment biosynthesis in Rhodobacter azotoformans

“…The growth rate and biomass of photoheterotrophic growing Rhodobacter azofoformans 134K20 were significantly decreased with the addition of glycine. These results were in accordance with those obtained from C. vinosum D [12] and R. sphaeroides MDC 6521 [13]. Kampf et al [12] demonstrated that the reason for this growth inhibition lied in a competition between glycine and D-or L-alanine for a substrate-binding site on an enzyme involved in peptidoglycan synthesis thus interfered with cell wall assembly, and the cells of growth inhibited cultures were misshaped.…”

“…As shown in Figure 2a, different concentrations of glycine could affect the 134K20 growth significantly. This was in consistent with the report that the growth rate of C. vinosum D decreased noticeably even at glycine concentrations of as low as 0.1 mM [12]. Figure 2b showed that the presence of 423 nm peak was dependent on glycine concentrations and this peak was disappeared at 15 mM glycine.…”

“…Lascelles [11] reported that glycine at the concentrations ranging from 0.25 to 10 mM promoted porphyrins (mainly coproporphyrin III) biosynthesis; however, excess glycine inhibited it. Kämpf et al [12] reported that glycine inhibited the phototrophic growth of Chromatium vinosum D and led to the growing cell's misshape. Gabrielyan et al [13] reported that the growth rate of Rhodobacter sphaeroides MDC 6521 grown on yeast-containing medium was negatively correlated with increased glycine concentration.…”