Inhibition of proinflammatory pathways by bioactive fraction of Tinospora cordifolia

Jacob, Jenny; Babu, Bashi M.; Mohan, Mohind C.; Abhimannue, Anu P.; B, Prakash Kumar

doi:10.1007/s10787-017-0319-2

Cited by 21 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of many pro-inflammatory stimulants, such as cytokines, LTs, infectious agents, or oxidative stress, activates NF-κB which thereby activates the expression of many inflammatory mediators such as enzymes, chemokines, and adhesion molecules. [ 23 24 ] The inflammatory mediators promote monocyte–macrophage differentiation, macrophage relocalization, at the site of infection leading to chronic inflammation. LT is an effective chemoattractant mediator of inflammation due to the presence of seven transmembrane-spanning G protein receptors.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer – An in vivo zebrafish study

et al. 2021

View full text Add to dashboard Cite

Background: The eugenol from zinc oxide eugenol (ZnOE) sealer tends to diffuse to the periapical region resulting in inflammation. Several modifications of ZnOE sealer have been formulated to minimize the inflammatory potential of the ZnOE sealer. Petasites hybridus contains petasin which possesses anti-inflammatory property used in treatment of migraine and allergic rhinitis. Aim: The aim of the study was to evaluate the anti-inflammatory property of petasin-incorporated ZnOE sealer on zebrafish. Materials and Methods: The study has been reviewed and approved by the Institutional Review Board (SRMU/MandHS/SRMDC/2018/S/025) and by the in-house Institutional Animal Ethics Committee (IAEC) of Pentagrit Research Lab and conducted with compliance to ICH harmonization and principles for animal housing and handling (IAEC Study No: 215/Go06/IAEC). The samples were implanted in the caudal portion of the zebrafish. The samples ( n = 50) were divided into five groups ( n = 10) – Group 1: incision only (negative control), Group 2: zinc oxide (ZnO) (positive control), Group 3: ZnO + eugenol and petasin in ratio of 10:1, Group 4: ZnO + eugenol and petasin in ratio of 5:1, and Group 5: ZnO + eugenol and petasin in ratio of 1:1. The experimental groups were further subdivided into two subgroups based on time intervals at 24 h and 48 h. The tissue samples were assessed using smear pathology test, and the percentage of inflammation was evaluated. The data were statistically analyzed using SPSS software version 22.0 with a significance level fixed as 5% (α = 0.05). Results: The presence of inflammatory cells and congestion of arterioles were taken as the criteria to assess inflammatory action. It was maximum in ZnOE sealer followed by ZnOE sealer modified with the addition of petasin to eugenol in the ratio of 10:1, 5:1, and 1:1, respectively. Conclusion: The addition of petasin extract to ZnOE reduces the inflammation potential of ZnOE sealers. ZnOE sealer containing eugenol and petasin in the ratio of 1:1 showed a maximal reduction in inflammation.

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer – An in vivo zebrafish study

et al. 2021

View full text Add to dashboard Cite

show abstract

“…cordifolia has established anti-inflammatory and immunomodulatory properties as observed in several experimental and clinical studies. [ 42 43 44 45 ] It modulates the response of CD4+ and CD 8+ T-cells, activates macrophages via TLR6 signaling and nuclear factor kappa B translocation, inhibit lipoxygenase, and cyclooxygenase enzymes, inhibit tumor necrosis factor-a and cytokine production and alters the level of enzymes such as catalase to stimulate lymphocytes. [ 42 43 44 45 ] T .…”

Section: Discussionmentioning

confidence: 99%

“…[ 42 43 44 45 ] It modulates the response of CD4+ and CD 8+ T-cells, activates macrophages via TLR6 signaling and nuclear factor kappa B translocation, inhibit lipoxygenase, and cyclooxygenase enzymes, inhibit tumor necrosis factor-a and cytokine production and alters the level of enzymes such as catalase to stimulate lymphocytes. [ 42 43 44 45 ] T . cordifolia also alters the activity of immune system through the dynamic regulation of cytokines.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of Guduchighana Vati in asymptomatic and mild-to-moderate cases of coronavirus disease-19: A randomized controlled pilot study

Rai

Shukla²,

Ujjaliya³

et al. 2020

AYU

View full text Add to dashboard Cite

Background: Currently, there is no approved treatment for the management of coronavirus disease (COVID-19). Drug repurposing of existing medications could be a possible way to find out a novel therapeutic entity to combat the COVID-19. Aim: To determine the clinical efficacy and safety of an Ayurveda intervention ( Guduchighana Vati ) in asymptomatic and mild-to-moderate cases of COVID-19. Materials and methods: This was an open-label randomized controlled pilot study with a sample size of 30 participants (15 in each arm). The participants were asymptomatic or mild to moderate cases of COVID-19. Guduchighana Vati 500 mg twice daily for 10 days was administered in the study group and Hydroxychloroquine for 5 days in the control group. Paracetamol, Vitamin C, Multivitamin, and Zinc were also provided in the control group. The main outcome measures were to negative real-time reverse transcription–polymerase chain reaction (RT-PCR) assay for COVID-19, proportion of participants with negative RT-PCR for COVID-19 at 5 th and 10 th day, proportion of participants with clinical recovery, improvement in laboratory parameters, and incidence of adverse drug reaction/adverse event (ADR/AE). The results of RT-PCR and clinical recovery were compared between groups using Chi-square test. The data related to laboratory parameters were compared within group using paired sample t -test/Wilcoxon signed-rank test and between groups using independent sample t -test/Mann–Whitney test. Results: The proportion of participants with negative RT-PCR for COVID-19 in the Guduchighana Vati group (93.3%) was better as compared to the control group (66.6%) till 10 th day of the study period. Though, the results are statistically not significant ( P = 0.068). All the symptomatic patients in the Guduchighana Vati group clinically recovered whereas one patient remained symptomatic in the control group on the 5 th day. No symptoms of COVID-19 were observed at 10 th day in both the groups. No ADR/serious adverse event were observed during the study period in either of the groups. Conclusion: In this study on asymptomatic and mild to moderate cases of COVID-19, Guduchighana Vati showed numerically better proportion of participants with negative RT-PCR assay for COVID-19 and reduced time to clinical improvement which requires confirmation through studies with larger sample size. Although, the study outcomes are statistically not significant which may be due to small sample size.

show abstract

“…Cell viability was measured by MTT assay according to protocol described earlier with slight modifications. [27] THP-1 cells were plated in a 96-well plate at a density of 1 × 10 4 cells/well. After 24 h of incubation, serial dilutions of (6.25, 12.5, 25, 50, and 100 μg/mL) fractions of Kashayam (dissolved in cell culture medium) were added and cells were incubated for next 24 h with 5% CO 2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of anti-inflammatory effect of Varanadi Kashayam (decoction) in THP-1-derived macrophages

Chinchu

Mohan

et al. 2018

AYU

View full text Add to dashboard Cite

Background: Varanadi Kashayam is an Ayurvedic polyherbal decoction containing 16 ingredients, for which the mechanisms of action involved in controlling chronic inflammatory conditions have not been evaluated. The inhibition of release of proinflammatory cytokines by lipopolysaccharide (LPS)-stimulated monocytes/macrophages is an ideal in vitro model for identifying anti-inflammatory molecules. Aim: The aim of the study is to determine the anti-inflammatory effect of Varanadi Kashayam in THP-1-derived macrophages. Materials and Methods: The efficacy of Varanadi Kashayam on monocyte cell differentiation was determined by quantitative polymerase chain reaction to assess the expression of differentiation markers MMP-9, CD36, CD11b and CD14. Further Varanadi Kashayam treated THP-1 macrophages were induced with LPS and the production of proinflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β) were measured and corresponding genes expressions were quantified. Results: The results indicate that Varanadi Kashayam reduced the differentiation of THP-1 monocytes to macrophages and downregulated the expression of cell surface markers. Furthermore, it could decrease the release of proinflammatory cytokines from LPS-induced THP-1 macrophages and downregulated the expression of TNF-α and IL-1β genes. Conclusion: The results obtained from this study suggest a possible mechanism of action of the herbal decoction in inflammatory processes and opens up the possibilities of identifying bioactive lead molecules with anti-inflammatory potentials.

show abstract

Inhibition of proinflammatory pathways by bioactive fraction of Tinospora cordifolia

Cited by 21 publications

References 25 publications

Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer – An in vivo zebrafish study

Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer – An in vivo zebrafish study

Efficacy and safety of Guduchighana Vati in asymptomatic and mild-to-moderate cases of coronavirus disease-19: A randomized controlled pilot study

Evaluation of anti-inflammatory effect of Varanadi Kashayam (decoction) in THP-1-derived macrophages

Contact Info

Product

Resources

About