Inhibition of Prostaglandin E2 Production by Platycodin D Isolated from the Root of Platycodon grandiflorum

Kim, Yong Pil; Lee, Eun Bang; Kim, Seung Yeon; Li, Dawei; Ban, Hyun Seung; Lim, Soon Sung; Shin, Kuk Hyun; Ohuchi, Kazuo

doi:10.1055/s-2001-14317

Cited by 108 publications

(63 citation statements)

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“…1 Platycodin saponins (PS) are the primary constituents of Platycodi Radix, 7 responsible for a diversity of effects including antiinflammation, antiallergy, antitumor, augmentation of immune response, and stimulating the apoptosis in skin cells. [8][9][10][11] In the last decade, PS has generated a renewed interest due to its pharmacological potentials for healing the diseases of adulthood such as hyperlipidemia, hypertension, diabetes, and obesity. 1,2 However, the evidence for the use of PS in obesity and hyperlipidemia treatment is few and sometimes discrepant.…”

Section: Introductionmentioning

confidence: 99%

Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction

Shim

et al. 2005

Int J Obes

123

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OBJECTIVE:To investigate how and to what extent platycodin saponin (PS) from Platycodi Radix exerts a favorable influence on obesity and hyperlipidemia. DESIGN: Sprague-Dawley rats were fed with a high fat (HF) diet for 4 weeks and then the animals were treated with 35 or 70 mg/kg of PS for another 4 weeks. Changes in body weight and daily calorie intake were measured regularly during the experimental period and the degree of linear correlation for the above two variables was further analyzed. The in vitro lipase inhibition of each PS compound and the in vivo fecal lipid excretion were examined in hope of revealing their relationship. The concentrations of hepatic triglyceride and cholesterol in serum. RESULTS: The body weight reduction (1374% vs HF control, Po0.05) by PS administration was highly correlated to the food intake restriction (Pearson's linear coefficient r ¼ 0.752, Po0.005). The in vitro inhibition of lipase by each isolated compound and mixture of PS were virtually identical. Consequently, the fecal TG excretion was increased by 2.1-3.2 folds depending on the dose of PS. The serum TG and LDL-cholesterol concentrations were decreased without noticeable changes in HDLcholesterol levels. Concomitantly, the contents of the hepatic TG, cholesterol, and the liver surface fat pads were decreased in ubiquity, but no noticeable biochemical abnormalities or histological tissue damages were observed. CONCLUSIONS: The administration of PS produced profound effects on the control of obesity and lipid metabolism, which resulted in LDL-cholesterol reduction. PS also caused a remarkable reduction in calorie intake, which was highly correlated to the body weight loss. These results suggest that PS has a greater role in antiobesity, hypolipidemia, and liver protection than previously thought. Hence, PS could be a potential therapeutic alternative in the treatment of obesity and hyperlipidemia.

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Section: Introductionmentioning

confidence: 99%

Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction

Shim

et al. 2005

Int J Obes

123

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“…Triterpene saponins, called platycosides, are responsible for the medicinal value of this plant material. They have demonstrated a broad spectrum of therapeutic effects, such as antitumor [4][5][6][7], anti-inflammatory [8,9], hepatoprotective [10], antiatherosclerotic [11] and antinociceptive [12]. Additionally, these compounds were found to be a potent adjuvant of specific cellular and humoral immune responses, with the potential use in vaccine production [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

et al. 2014

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IntroductionHairy, or transgenic roots are characterized by a rapid and stable growth, which importantly can be initiated and maintained in a hormone-free medium. They often synthetize approximately the same and in some cases even greater amounts of secondary metabolites compared to their mother plants [1,2] and the rate of production remains stable. This makes hairy roots of medicinal plants a good source of valuable compounds and obviously their large-scale culture in bioreactors holds immense potential for the pharmaceutical industry [3].Platycodon grandiflorum (Jacq.) A. DC. (Campanulaceae) is an ornamental plant growing wild in northern Asia, in China, Korea, Japan and east Siberia. For culture it needs light, medium moisture, organically rich, well-drained loams and full sun to part shade.Platycodi radix, the root of P. grandiflorum, has been used as food and in traditional oriental medicine to treat bronchitis, asthma and other pulmonary diseases. Triterpene saponins, called platycosides, are responsible for the medicinal value of this plant material. They have demonstrated a broad spectrum of therapeutic effects, such as antitumor [4][5][6][7], anti-inflammatory [8,9], hepatoprotective [10], antiatherosclerotic [11] and antinociceptive [12]. Additionally, these compounds were found to be a potent adjuvant of specific cellular and humoral immune responses, with the potential use in vaccine production [13][14][15]. Finally, saponins from Platycodi radix stimulated osteoblast differentiation via RUNX2, a principal osteogenic master gene for bone formation [16].Although the extensive pharmacological studies of these valuable compounds were undertaken, to date the sum of saponins, not the chosen, in hairy root cultures of P. grandiflorum was determined only by our research team [17]. Besides, this is the first report of the growth of P. grandiflorum transgenic roots cultured in a mist bioreactor and their yield of saponins. The method of Platycodi radix saponins determination published in "Chinese pharmacopeia" [18] exhibited as no suitable for quantitative analysis of saponins from P. grandiflorum transgenic roots. Therefore, the authors elaborated their own method [17].The compounds mentioned above are the main chemicals found in Platycodi radix. They are typical composed of oleaene backbones with two side chains: one is a 3-O-glucose linked by glycosidic bond, and the other is a 28-O-arabinoserhamnose-xylose-apiose linked by an ester bond [19]. To date, more than 55 triterpenoid saponins have been isolated from Platycodi radix [20]. Examples are platycodins A-I and polygalacins D and D2. According to WHO monographs AbstractThe growth and saponin accumulation were measured in two lines of transgenic hairy roots of Platycodon grandiflorum, Pl 6 and Pl 17, cultured for 8 weeks in 250-ml shake flasks containing 50 ml of hormone-free woody plant medium supplemented with 40 g/l sucrose and in the Pl 17 line cultured for 12 weeks in a 5-l mist bioreactor containing 1.5 l of the same medium. With both methods, the...

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“…According to traditional oriental medicine, the root of Platycodon grandiflorum has been used for regulating pulmonary inflammatory diseases (Jang, 2003). Platycodin D (PD), a compound isolated from the root of Platycodon grandiflorum , was reported to have various biological effects including anticancer effect (Kim et al, 2001;Ahn et al, 2006;Chung et al, 2008;Kim et al, 2008;Yu and Kim, 2010). Platycodin D induced apoptosis in human breast cancer cells (Yu and Kim, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Lee¹,

Lee²,

Jeon³

et al. 2010

Biomolecules and Therapeutics

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-In this study, we tried to investigate whether platycodin D significantly affects MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF), phorbol ester (PMA) and tumor necrosis factor-α (TNF-α) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of platycodin D for 30 min and then stimulated with EGF, PMA and TNF-α for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) Platycodin D was found to inhibit the production of MUC5AC mucin protein induced by EGF, PMA, and TNF-α, respectively. (2) It also inhibited the expression of MUC5AC mucin gene induced by the same inducers. These results suggest that platycodin D can regulate mucin gene expression and production of mucin protein, by directly acting on human airway epithelial cells.

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Inhibition of Prostaglandin E2 Production by Platycodin D Isolated from the Root of Platycodon grandiflorum

Cited by 108 publications

References 0 publications

Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction

Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction

The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

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