Inhibition of Protein Synthesis and Cation Uptake in Beetroot Tissue by Cycloheximide and Cryptopleurine

Abstract

The effects of cycloheximide and of cryptopleurine (two inhibitors of protein synthesis) on ion uptake and protein synthesis in beet disks are described

Influence of Calcium on Sodium and Potassium Absorption by Fresh and Aged Bean Stem Slices

“…The development of ion absorption capacity along with increased respiration has been suggested to involve basic alterations in physiological processes of plant tissue (1,9,14,20,40) and especially in protein metabolism (4,12,20,27). Figure 9A shows results of an experiment on the effect of cycloheximide, a known inhibitor of protein synthesis (4), on the development of K absorption capacity.…”

“…It has been demonstrated that by washing slices of aging tuberous roots or stem tissues in various solutions, ion absorption (1,2,15,17,21,30,33,(36)(37)(38), respiratory patterns (14,15,17,19,26,33,38), endoplasmic reticulum (9), protein and RNA metabolism (3,4,12,20,27), and phospholipid turnover in membrane fractions (40) are altered. Plant hormones have also been found to influence the response of isolated tissues as they are aged (30,34,39).…”

“…A review of the literature indicated that one of the variables in such investigations was the solution in which the tissue was aged. For (1,17) and in solutions consisting of tap water (19,21), tris-buffer (36,37), and various salts (2,17,27,30,40).…”

Action of Protein Synthesis Inhibitors in Blocking Electrogenic H+ Efflux from Corn Roots

“…The It has been repeatedly demonstrated in a wide variety of plant tissues that inhibitors of protein synthesis, such as cycloheximide, will also inhibit energy-linked ion absorption, but the nature of this inhibition is not resolved. In tissues where ion-absorption rates are increasing due to washing or aging (13,17,25,26), the inhibition might be reasonably attributed to blocked synthesis of those enzymes required to augment transport rates. In other cases, however, there have been suggestions that protein synthesis inhibitors may have secondary or side effects, possibly interfering with metabolic energy supply (9,14,22 tions, also blocked IAA-induced growth and its associated increases in respiration, H+ efflux, and cell potential.…”

“…In tissues where ion-absorption rates are increasing due to washing or aging (13,17,25,26), the inhibition might be reasonably attributed to blocked synthesis of those enzymes required to augment transport rates. In other cases, however, there have been suggestions that protein synthesis inhibitors may have secondary or side effects, possibly interfering with metabolic energy supply (9,14,22).…”