Inhibition of protein synthesis by cycloheximide in rabbit reticulocytes

Colombo, Bruno; Felicetti, L.; Baglioni, Corrado

doi:10.1016/0006-291x(65)90719-9

Cited by 160 publications

(48 citation statements)

References 5 publications

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“…Sodium fluoride and cycloheximide suppress protein synthesis by inhibiting the initiation complex formation and peptide chain elongation on ribosome, respectively (Colombo et al 1965;Ravel et al 1966). HB4C5 cells were pre-cultured for 6 h in ITES-ERDF medium containing 4.0 mM NaF or 10.0 g/ml of cycloheximide before the IPSF assay.…”

Section: Inhibition Of Protein Synthesismentioning

confidence: 99%

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

Takenouchi

Sugahara

2003

Cytotechnology

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Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 g/ml under serum-free conditions. LDHs from pig muscle and pig heart also accelerated IgM production 8.4-and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied by activation of cell proliferation. LDH from rabbit muscle facilitated IgM and IgG production by human peripheral blood lymphocytes. This means LDH stimulates immunoglobulin production not only by the specified hybridoma cell line, but also by unspecified immunoglobulin producers. LDH from rabbit muscle enhanced IgM production of transcription-suppressed HB4C5 cells treated with actinomycin D. The immunoglobulin production-stimulating factors (IPSFs) effect of LDH was slightly weakened by sodium fluoride (translation inhibitor) treatment of HB4C5. Moreover, the amount of intracellular IgM of monensin-treated HB4C5 cells was obviously enhanced by LDH. This result means that the IPSF effect of LDH is irrelevant to the post-translation activity of target cells. It is expected from these findings that LDH from rabbit muscle accelerates the translation step to enhance immunoglobulin productivity. The immunoglobulin production-stimulating activity of LDH was inhibited by colchicine, endocytosis inhibitor. This fact suggests that it is necessary for LDH to be taken by target cells to act as an IPSF.

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Section: Inhibition Of Protein Synthesismentioning

confidence: 99%

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

Takenouchi

Sugahara

2003

Cytotechnology

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“…Specificity was also observed between the antigen used for preparation of immune RNA and the antibody detected on spleen cells. The increment of number of rosette formers after treatment with immune RNA was inhibited by metabolic inhibitors of protein synthesis at translation stage [7,21]. These results indicate that the cells, which are treated with RNA fraction extracted from spleen of immunized mice, do produce specific antibody.…”

Section: Effect Of Metabolic Inhibitors On the Production Of Rosette-mentioning

confidence: 80%

Transfer Agent of Immunity

Kawakami

Kïtamura

Mikami

et al. 1969

Japanese Journal of Microbiology

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Using erythrocytes as antigen particles, number of antibody‐forming cells was enumerated by immunocytoadehesion technique, in which formation of rosette was shown to be inhibited by anti‐mouse immunoglobulin sera. This number increased in vitro after treatment of spleen cells of mice for 60 min with RNA fraction extracted from spleen of mice immunized with erythrocytes used in the enumeration, and incubation of cells for 12 hr at 37 C. Response of cells treated with immune RNA fraction was immunologically specific and was inhibited by puromycin or cycloheximide. The activity of immune RNA capable of converting nonimmune cells to antibody‐forming cells was shown to be sensitive to ribonucleases but resistant to deoxyribonuclease and proteolytic enzyme.

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“…The mechanism of action for cycloheximide has been reported as the direct prevention of ribosomal movement along the m-RNA chains (Wettstein, Noll & Penman, 1964;Colombo, Felicetti & Boglioni, 1965). Therefore, it might be reasoned that the induction of tolerance to the depletion of calcium may in some way be connected to the translational aspect of protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Tolerance to Morphine‐induced Calcium Depletion in Regional Brain Areas: Characterization With Reserpine and Protein Synthesis Inhibitors

Ross

1975

British J Pharmacology

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Administration of a single dose of morphine sulphate (25 mg/kg) induces tolerance to calcium depletion lasting seven days. There are no apparent changes in calcium content in any of eight discrete brain regions throughout this seven day period. Pretreatment with reserpine (5 mg/kg) did not alter the ability of morphine to induce tolerance. Reserpine alone produced no tolerance to its own calcium depleting action. Cycloheximide (500 μg/kg) but not chloramphenicol (200 mg/kg) effectively prevented development of tolerance. It is concluded that the induction of tolerance to calcium depletion seen after morphine may involve changes in various proteins in membranes of synaptic origin.

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Inhibition of protein synthesis by cycloheximide in rabbit reticulocytes

Cited by 160 publications

References 5 publications

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes

Transfer Agent of Immunity

Tolerance to Morphine‐induced Calcium Depletion in Regional Brain Areas: Characterization With Reserpine and Protein Synthesis Inhibitors

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