Inhibition of protein thiol modification in hepatocytes isolated from rats supplemented with vitamin E under oxidative stress

Lii, Chong‐Kuei; Chen, H.-W.; Wang, S.-T.

doi:10.1016/s0887-2333(96)00048-3

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Other functions of vitamin E have also been proposed, e.g. in maintaining membrane protein thiols (Takenaka et al, 1991), in stabilising membrane structure (Urano et al, 1992), in protecting cells with other antioxidants from damage and lysis induced by oxidative stress (Konar et al, 2000), in preventing certain diseases (Lii et al, 1996) and in activating fish immune functions (Anderson, 1992;Sakai, 1999).…”

Section: Abstract: Carbonic Anhydrase Erythrocyte Vitamin E Troutmentioning

confidence: 99%

Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Aras-Hisar

Hisar

Beydemır

et al. 2004

Acta Veterinaria Hungarica

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Considering that the excessive usage of vitamin E causes hypervitaminosis and thus reduces blood erythrocyte concentrations, therefore it is worth studying how its pharmacological dosage affects the activity of carbonic anhydrase (CA) enzyme found in erythrocytes of rainbow trout (Oncorhynchus mykiss) in vitro and in vivo. Vitamin E inhibited CA enzyme and the IC 50 value of the vitamin was 0.039 mM in vitro. Similarly, it was seen that vitamin E inhibited CA enzyme activity after the first hour following vitamin E injections in vivo. The activities of CA in groups of trout given vitamin E injection were measured at 1, 3 and 5 h and the corresponding activities were found to be 772.7 ± 290.5 (P < 0.05), 1286.4 ± 378.2 and 1005.7 ± 436.1 enzyme units (EU) g Hb -1 . The difference over the control was significant (P < 0.05) in the first hour and insignificant at 3 and 5 h (P > 0.05). The activity of CA in the control, which did not contain vitamin E, was determined as 1597.7 ± 429.0 EU g Hb -1 .

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Section: Abstract: Carbonic Anhydrase Erythrocyte Vitamin E Troutmentioning

confidence: 99%

Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Aras-Hisar

Hisar

Beydemır

et al. 2004

Acta Veterinaria Hungarica

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“…Other functions of α-tocopherol have also been proposed, e.g. in maintaining membrane protein thiols, stabilising membrane structure, preventing certain diseases (Lii et al, 1996), activating fish immune functions (Anderson, 1992;Sakai, 1999) and protecting cells with other antioxidants from damage and lysis induced by oxidative stress (Konar et al, 2000).…”

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confidence: 99%

Effect of α-tocopherol on antioxidant enzyme activities and lipid peroxidation in rainbow trout (Oncorhynchus mykiss)

Gülçın

Beydemır²,

Hisar³

2005

Acta Veterinaria Hungarica

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In this study, the effect of α-tocopherol on the antioxidant capacity of rainbow trout (Oncorhynchus mykiss) was determined. For this purpose, activities of antioxidant enzymes such as catalase (CAT), peroxidase (POD) and glutathione reductase (GSSG-Rx) were investigated. Enzyme activities were measured at 0 (control), 1, 3 and 5 h after α-tocopherol injection. In addition, the levels of malondialdehyde (MDA) as a lipid peroxidation marker were determined in the erythrocytes. The results showed that α-tocopherol significantly activated the CAT, POD and GSSG-Rx enzymes as compared with the enzyme activities found in the controls (p < 0.05). However, MDA levels were significantly decreased by α-tocopherol treatment (p < 0.05). The results suggest that α-tocopherol may have a pro-oxidant tendency at a high dose and cause mild oxidative stress which could modulate signal transduction cascades, redirect gene expression, and influence many cellular responses such as proliferation, differentiation, and reproduction. For this reason, α-tocopherol should be used carefully in all applications in relation to fish.

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Sublethal ammonia and urea concentrations inhibit rainbow trout (Oncorhynchus mykiss) erythrocyte glucose-6-phosphate dehydrogenase

Erdoğan

Hisar

Köroğlu

et al. 2005

Comparative Biochemistry and Physiology Part C: Toxicology &

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Inhibition of protein thiol modification in hepatocytes isolated from rats supplemented with vitamin E under oxidative stress

Cited by 4 publications

References 29 publications

Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Effect of α-tocopherol on antioxidant enzyme activities and lipid peroxidation in rainbow trout (Oncorhynchus mykiss)

Sublethal ammonia and urea concentrations inhibit rainbow trout (Oncorhynchus mykiss) erythrocyte glucose-6-phosphate dehydrogenase

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