Inhibition of protein tyrosine phosphatase 1B and regulation of insulin signalling markers by caffeoyl derivatives of chicory (Cichorium intybus) salad leaves

Abstract

Evaluations of molecular mechanisms of dietary plants with their active molecules are essential for the complete exploration of their nutritive and therapeutic value. In the present study, we investigated the effect of chicory (Cichorium intybus) salad leaves in inhibiting protein tyrosine phosphatase 1B (PTP1B), and evaluated their role in modulating the key markers involved in insulin cell signalling and adipogenesis using 3T3-L1 adipocytes. Bioactivity-directed purification studies enlightened the additive effects of chlorogenic acid (CGA) along with other caffeic acid derivatives present in methanolic extract of C. intybus (CME). Incubation of CME and CGA with 3T3-L1 adipocytes significantly enhanced the 2-deoxy-D- 3 [H]-glucose uptake and inhibited adipogenesis through altering the expressions of insulin signalling and adipogenesis markers. Extending to an in vivo model, the effect of CME was also investigated on insulin sensitivity in high-fat diet with low streptozotocin-induced diabetic rats. Supplementation of CME for 2 weeks reinstated the insulin sensitivity along with plasma metabolic profile. The present results demonstrate that the caffeoyl derivatives of chicory salad leaves show promising pharmacological effect on energy homoeostasis via PTP1B inhibition both in vitro and in vivo.

Absorption and Metabolism of Chlorogenic Acids in Cultured Gastric Epithelial Monolayers

“…NO availability (Rocha et al, 2009), and inhibition of inflammatory factors (Chu et al, 2011). Coffee contains many potentially bioactive compounds, and there is growing evidence that CGAs and hydroxycinnamic acid derivatives may play an important role in glucose metabolism through modulation of glucose absorption (Johnston et al, 2003), insulin sensitivity (Muthusamy et al, 2010), and inhibition of glycogenolysis (Andrade-Cetto and Vázquez, 2010;Henry-Vitrac et al, 2010). Despite the potential pharmacological effects of CGAs, there is limited knowledge concerning bioavailability and the mechanism of gastrointestinal absorption.…”

Medicinal Plants from Jordan in the Treatment of Diabetes: Traditional Uses vs.In VitroandIn VivoEvaluations – Part 2

“…A guaianolide sesquiterpene glycoside, cichotyboside [76]. Tannins, chlorogenic acid along with caffeic acid derivatives [77] Antihyperglycemic effect in alloxan-induced diabetes mice [78] and clinically proven reduction on diabetes risk [79], which can be explained by a reduction in intestinal absorption of glucose [80]. Significant increases in catalytic concentration of glutathione S-transferases in the liver of diabetic NOD mice [81].…”

“…Significant increases in catalytic concentration of glutathione S-transferases in the liver of diabetic NOD mice [81]. Its dietary inulin-type fructans promote secretion of endogenous gastrointestinal peptides involved in appetite regulation [82] and via reduction in the hepatic Glc-6-pase activity thus decreasing hepatic glucose production, with no possibility of inducing insulin secretion from pancreatic beta-cells [83], also through adipogenesis inhibition and PPARgamma upregulation, inhibition of protein tyrosine phosphatase 1B and regulation of insulin signalling markers [77,84]. α-Glucosidase inhibition [85].…”

Pre-meal tomato (Lycopersicon esculentum) intake can have anti-obesity effects in young women?

“…and decrease rat body weight and abdominal fat pad weight (Andrade-Cetto & Vázquez, 2010;Kim et al, 2011;Muthusamy et al, 2010). Feeding rats with foods rich in the flavonol glycoside rutin decreased their weight, number of fat cells, insulin, leptin, hepatic triacylglycerol, cholesterol, with an improved overall serum lipid profile (Hsu et al, 2009;Rickman et al, 2010).…”