Inhibition of Pseudomonas aeruginosa quorum sensing by methyl gallate from Mangifera indica

Naga, Nourhan G.; Zaki, Ahmed A.; El-Badan, Dalia E.; Rateb, Heba S.; Ghanem, Khaled M.; Shaaban, Mona I.

doi:10.1038/s41598-023-44063-0

Cited by 11 publications

(2 citation statements)

References 69 publications

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“…The use of natural compounds as antivirulence and anti-QS agents was previously reported. Methyl gallate showed a strong inhibition of QS in P. aeruginosa strains PAO1 and PA14 at ¼ MICs without affecting bacterial viability ( Naga et al., 2023 ). Moreover, flavanones, naringenin, eriodictyol, and taxifolin extracted from Combretum albiflorum interfered with pyocyanin production and elastase activity in P. aeruginosa without impacting bacterial growth.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone is a natural antibiofilm and pathogenicity attenuating agent in Pseudomonas aeruginosa

Al-Rabia,

Asfour,

Alhakamy

et al. 2024

Front. Cell. Infect. Microbiol.

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Pseudomonas aeruginosa belongs to the critical pathogens that represent a global public health problem due to their high rate of resistance as listed by WHO. P. aeruginosa can result in many nosocomial infections especially in individuals with compromised immune systems. Attenuating virulence factors by interference with quorum sensing (QS) systems is a promising approach to treat P. aeruginosa-resistant infections. Thymoquinone is a natural compound isolated from Nigella sativa (black seed) essential oil. In this study, the minimum inhibitory concentration of thymoquinone was detected followed by investigating the antibiofilm and antivirulence activities of the subinhibitory concentration of thymoquinone against P. aeruginosa PAO1. The effect of thymoquinone on the expression of QS genes was assessed by quantitative real-time PCR, and the protective effect of thymoquinone against the pathogenesis of PAO1 in mice was detected by the mouse survival test. Thymoquinone significantly inhibited biofilm, pyocyanin, protease activity, and swarming motility. At the molecular level, thymoquinone markedly downregulated QS genes lasI, lasR, rhlI, and rhlR. Moreover, thymoquinone could protect mice from the pathologic effects of P. aeruginosa increasing mouse survival from 20% to 100%. In conclusion, thymoquinone is a promising natural agent that can be used as an adjunct therapeutic agent with antibiotics to attenuate the pathogenicity of P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Thymoquinone is a natural antibiofilm and pathogenicity attenuating agent in Pseudomonas aeruginosa

Al-Rabia,

Asfour,

Alhakamy

et al. 2024

Front. Cell. Infect. Microbiol.

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“…The fourth is the AI-based integrated quorum sensing system. 24 In particular, QS blockade abrogates the virulence without affecting bacterial growth. Therefore, QS represents an attractive target for anti-virulence therapy.…”

Section: Discussionmentioning

confidence: 99%

Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors inPseudomonas aeruginosa

González,

García Contreras,

García Valdés

et al. 2024

Preprint

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ObjetiveTo evaluate the inhibitory effect of Quorum Sensing-dependent virulence factors inPseudomonas aeruginosaby 2 molecules; curcumin and 5-bromoindole-3-carboxyaldehyde.MethodsFor the tests, 3 strains ofP. aeruginosawere used; Pa01, Pa14 and ATCC 27853. The strains were reseeded in LB medium boxes for 12 hours at 37°. ON cultures of each strain were inoculated in LB broth, grown for 24 hours at 37°. Subsequently, new cultures of the strains were inoculated at starting from the ON cultures with an initial O.D 600 nm of approximately 0.0125, the following concentrations of the molecules were evaluated with these cultures: curcumin (1 and 5 μg/ml) and 5-bromoindole-3-carboxyaldehyde (30, 50 and 100 μg/ml). The factors were read virulence with their respective wavelength; protease (595 nm), elastase (495 nm), pyocyanin (520 nm) and alginate (530 nm).Results5-bromoindole-3-carboxyaldehyde significantly inhibited pyocyanin production in Pa01 (92.5% at 100 μg/ml), elastase in Pa01 (79.85% at 100 μg/ml), protease in Pa01 (92.8% at 50 μg/ml), and alginate in ATCC 27853 (76.16% at 100 μg/ml). Curcumin showed substantial inhibition of pyocyanin in ATCC 27853 (100% at 5 μg/ml), elastase in Pa14 (56.19% at 5 μg/ml), protease in Pa01 (87.04% at 5 μg/ml), and alginate in Pa01 (48.26% at 5 μg/ml).ConclusionsCurcumin and 5-bromoindole-3-carboxyaldehyde demonstrated effective inhibition of QS-dependent virulence factors inP. aeruginosa. These findings suggest the potential of these compounds as anti-virulence agents, providing a promising alternative to conventional antibiotics in combatingP. aeruginosainfections.

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Role of fatty acids in modulating quorum sensing in Pseudomonas aeruginosa and Chromobacterium violaceum: an integrated experimental and computational analysis

Gopalakrishnan,

Kanagaraja,

Sakthivelu

et al. 2024

Int Microbiol

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Inhibition of Pseudomonas aeruginosa quorum sensing by methyl gallate from Mangifera indica

Cited by 11 publications

References 69 publications

Thymoquinone is a natural antibiofilm and pathogenicity attenuating agent in Pseudomonas aeruginosa

Thymoquinone is a natural antibiofilm and pathogenicity attenuating agent in Pseudomonas aeruginosa

Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors inPseudomonas aeruginosa

Role of fatty acids in modulating quorum sensing in Pseudomonas aeruginosa and Chromobacterium violaceum: an integrated experimental and computational analysis

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