Inhibition of quorum sensing in Pseudomonas aeruginosa by sesquiterpene lactones

Amaya, Susana; Pereira, José Aires; Borkosky, Susana; Valdéz, Juan Carlos; Bardón, Alicia; Arena, Mario Eduardo

doi:10.1016/j.phymed.2012.07.003

Cited by 65 publications

(42 citation statements)

References 28 publications

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“…For instance, some terpenoids inhibit: (i) the morphogenesis, adhesion, and biofilm formation in Candida albicans as the example of linalool and farnesol [39]; (ii) the biofilm formation and elastase production in P. aeruginosa and Staphylococcus aureus as the example of viridiflorol, ursolic and betulinic acids [40]; or (iii) the production of native auto-inducers acylhomoserine lactones in P. aeruginosa [41] and the QS-controlled violacein production in C. violaceum as the example of the sesquiterpene lactones derivatives [42]. …”

Section: Discussionmentioning

confidence: 99%

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Rasamiravaka

Ngezahayo

Pottier

et al. 2017

IJMS

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Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine.

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Section: Discussionmentioning

confidence: 99%

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Rasamiravaka

Ngezahayo

Pottier

et al. 2017

IJMS

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“…It was suggested that farnesol may play a role in interkingdom communications [97]. Besides farnesol, two other sesquiterpene derivatives, drimendiol from Drimys winteri and sesquiterpene lactones from Centratherum punctatum (Argentine herb), were identified as AHL-dependent QS inhibitors [83,87]. Referring to the AI-2 system, furanone, cinnamaldehyde and their derivatives may be the most effective inhibitors.…”

Section: Natural Quorum Quenching Resourcesmentioning

confidence: 99%

Quorum Quenching Agents: Resources for Antivirulence Therapy

2014

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The continuing emergence of antibiotic-resistant pathogens is a concern to human health and highlights the urgent need for the development of alternative therapeutic strategies. Quorum sensing (QS) regulates virulence in many bacterial pathogens, and thus, is a promising target for antivirulence therapy which may inhibit virulence instead of cell growth and division. This means that there is little selective pressure for the evolution of resistance. Many natural quorum quenching (QQ) agents have been identified. Moreover, it has been shown that many microorganisms are capable of producing small molecular QS inhibitors and/or macromolecular QQ enzymes, which could be regarded as a strategy for bacteria to gain benefits in competitive environments. More than 30 species of marine QQ bacteria have been identified thus far, but only a few of them have been intensively studied. Recent studies indicate that an enormous number of QQ microorganisms are undiscovered in the highly diverse marine environments, and these marine microorganism-derived QQ agents may be valuable resources for antivirulence therapy.

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“…Lactones with a p ‐menthane system have exhibited activity against stored‐product pests, aphids, lesser mealworm and Colorado potato beetle . Additionally, this group of compounds is known for a wide spectrum of antibacterial activity . They can have different mechanisms of action, for example inhibition of bacterial growth or reduction in biofilm formation .…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, this group of compounds is known for a wide spectrum of antibacterial activity . They can have different mechanisms of action, for example inhibition of bacterial growth or reduction in biofilm formation . Taking into consideration the low concentrations of these compounds in plants, their isolation from natural materials is uneconomical.…”

Section: Introductionmentioning

confidence: 99%

“…16 -19 They can have different mechanisms of action, for example inhibition of bacterial growth 16,18 or reduction in biofilm formation. 19 Taking into consideration the low concentrations of these compounds in plants, their isolation from natural materials is uneconomical. On the other hand, plant pathogens continually develop resistance to plant protection compounds or antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

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Lactones 46. Synthesis, antifeedant and antibacterial activity ofγ-lactones with ap-methoxyphenyl substituent

et al. 2015

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The results of biological tests showed that halogen atom removal significantly increased the antifeedant properties of γ-lactones with a p-methoxyphenyl substituent. Unsaturated lactones are most promising in the context of their possible industrial application as crop protection agents. Further structural modifications of lactones with aromatic rings are needed to find important structural factors increasing the antibacterial activity.

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Inhibition of quorum sensing in Pseudomonas aeruginosa by sesquiterpene lactones

Cited by 65 publications

References 28 publications

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Quorum Quenching Agents: Resources for Antivirulence Therapy

Lactones 46. Synthesis, antifeedant and antibacterial activity ofγ-lactones with ap-methoxyphenyl substituent

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