Inhibition of Rat Bladder Tumor (Rbt323) Growth by Tumor Necrosis Factor Alpha and Interferon-Gamma in Vivo

Moorselaar, R. Jeroen A. van; Hendriks, Ben Th.; Borm, George F.; Meide, P. H. Van Der; Debruyne, F.M.J.; Schalken, Jack A.

doi:10.1016/s0022-5347(17)36628-4

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…In this study, multiple injections of 20,000 units of IFN--y were given at the wound site between 4 and 29 days PW. The dosing regimen of IFN-y was derived from a study showing that peritumoral s.c. injections of 80,000 units, 3 times a week, inhibit tumor growth in rats (Van Moorselaar et al, 1992). The dose used in our study is considerably lower than the systemic dose used in a murine model (Granstein et al, 1989).…”

Section: Discussionmentioning

confidence: 69%

Local Injection of IFN-gamma Reduces the Number of Myofibroblasts and the Collagen Content in Palatal Wounds

et al. 2000

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Wound contraction and scar formation after cleft palate surgery impair maxillary growth and dentoalveolar development. Since myofibroblast numbers and scar formation are reduced by interferon-gamma (IFN-gamma) in the healing of dermal wounds, the hypothesis was tested that local administration of IFN-gamma reduces the numbers of myofibroblasts and the elevated amount of collagen during palatal mucoperiosteal wound healing. Standardized mucoperiosteal excision wounds were made in the palatal mucoperiosteum of young rats. Either IFN-gamma or vehicle alone (sham group) was repeatedly injected into the wound site between 4 and 29 days post-wounding. The results were compared with unmanipulated control wounds. Samples of wound tissue were prepared for biochemical and microscopic analysis. The hydroxyproline, sulfated glycosaminoglycan and DNA contents of the wound tissues were analyzed biochemically. The degree of re-epithelialization, tissue thickness, the numbers of myofibroblasts, and the amounts of elastin and collagen types I and III were evaluated on histological sections. Injection of vehicle alone affected almost all healing parameters, compared with the controls, and delayed the wound-healing process. IFN-gamma stimulated re-epithelialization and decreased the numbers of myofibroblasts when compared with vehicle-treated wounds. It also decreased the hydroxyproline and glycosaminoglycan contents of 60-day-old wound tissue, but the histological characteristics of scar tissue persisted. Therefore, IFN-gamma is able to reduce the numbers of myofibroblasts and the collagen content of scar tissue after palatal wound healing. It may be a promising pharmaceutical agent for the reduction of wound contraction and scarring after cleft palate surgery.

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Section: Discussionmentioning

confidence: 69%

Local Injection of IFN-gamma Reduces the Number of Myofibroblasts and the Collagen Content in Palatal Wounds

et al. 2000

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“…The RBT323 tumor is a non-carcinogen-induced tumor and arose spontaneously in an ACI rat. In the past, this RBT323 tumor was used as a subcutaneous rat bladder tumor model in our laboratory [ 31 , 32 ]. Histologically, the RBT323 tumor is a grade 2–3 pure urothelial cell tumor with no squamous cells and the RBT323 cell line closely resembles human non-muscle invasive UCC both phenotypically and cytogenetically [ 11 ].…”

Section: Potential New Orthotopic Aci Rat Bladder Tumor (Rbt323) Modementioning

confidence: 99%

Experimental rat bladder urothelial cell carcinoma models

et al. 2009

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Bladder cancer is a major public health problem. Currently available therapeutic options seem to be unable to prevent bladder cancer recurrence and progression. To enable preclinical testing of new intravesical therapeutic agents, a suitable bladder tumor model that resembles human disease is highly desirable. The aim of this topic paper was to discuss the problems associated with current in vivo animal bladder tumor models, focusing on the orthotopic syngeneic rat bladder tumor model. In the second part of the paper the development of a potential new orthotopic rat bladder tumor model is described.

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“…The tumor burden of TNFtreated mice was smaller or equal compared to rhIL-1 pitreated mice. TNF increased the survival of tumor-bear ing mice [63], hit rat amoral or Locoregional Treatment with TNF In the mouse bladder tumor model, MTB-2, intravesi cal treatment with TNF alone together with actinoinycin D showed no significant results [41], In a new nonimmunogenic rat bladder tumor model, RBT323, TNF showed dose-dependent and in combination with IFN-y synergis tic growth-inhibiting effects by administration peritumorally, and prolonged the time to tumor recurrence [64], In a rat model for colon adenocarcinoma, tumor frag ments implanted subcutaneously or under the hepatic capsule were treated with TNF i.v. or i.t.…”

Section: Intraperitoneal Tnfmentioning

confidence: 99%

Tumor Necrosis Factor for the Treatment of Malignancies

Hieber¹,

Heim

1994

Oncology

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Tumor necrosis factor (TNF) is a cytokine produced in vivo by activated macrophages and monocytes with pleiotropic effects on normal and malignant cells. TNF is cytotoxic to several tumor cell lines in vitro and in vivo. Phase I and phase II trials have been conducted to determine toxicity to humans and to evaluate responses. Recent investigations will be reviewed. Despite promising results in vitro and in vivo, data from systemic administration in the treatment of malignancies have been disappointing. Local administration has been successful. Therefore, we suggest that future efforts concerning TNF administration in the treatment of malignancies should aim at local treatment.

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Inhibition of Rat Bladder Tumor (Rbt323) Growth by Tumor Necrosis Factor Alpha and Interferon-Gamma in Vivo

Cited by 6 publications

References 21 publications

Local Injection of IFN-gamma Reduces the Number of Myofibroblasts and the Collagen Content in Palatal Wounds

Local Injection of IFN-gamma Reduces the Number of Myofibroblasts and the Collagen Content in Palatal Wounds

Experimental rat bladder urothelial cell carcinoma models

Tumor Necrosis Factor for the Treatment of Malignancies

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