Inhibition of Rat Heart Phosphodiesterase by some Rotenoids and Isoflavonoids

Petkov, E; Uzunov, P; Kostova, Irena; Somleva, T.; Ognyanov, I.

doi:10.1055/s-2007-969996

Cited by 17 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five-micron-thick sections, including the epidermis, the dermis, and the subcutaneous panniculus carnosus muscle, were mounted on glass slides, dewaxed, rehydrated in distilled water, and stained with hematoxylin and eosin(HE). All subsequent analyses were performed by an experienced pathologist without knowledge (Petkov et al, 1983;Li et al, 1993). The results of present study using wound healing experiments in animal models, biochemical analysis of wound biopsy, and in vitro studies showed that three different compounds isolated from the fruits of Amorpha fruticosa L. have antibacterial activity and promote the healing of wounds.…”

Section: Histological Examinationmentioning

confidence: 69%

Evaluation of anti-bacterial and wound healing activity of the fruits of <i>Amorpha fruticosa l.</i>

Qu¹,

Diao²,

Zhang³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

As the traditional Chinese medicine, the fresh fruits of Amorpha fruticosa L. were applied for the treatment of carbuncle, eczema and burn (Das et al., 2007). However, little is known about the functional roles of the fruits of Amorpha fruticosa L. during wound healing progress. In the present study, we evaluated both antimicrobial potential against a wide range of microorganisms and wound healing activity of the seven compounds isolated from the fruits of Amorpha fruticosa L in vitro and in vivo. Our results showed that compounds I (6a,12a-dehydroamorphin), V (dehydrosermundone) and VI (tephrosin) isolated from the fruits of Amorpha fruticosa L. performed dominant antimicrobial potential against microorganisms. Moreover, these compounds significantly enhanced fibroblasts proliferation and migration, leading to promotion of wound healing. Thus, it could be possible for the therapeutic utilization of Amorpha fruticosa L. for wound healing in the future.

show abstract

Section: Histological Examinationmentioning

confidence: 69%

Evaluation of anti-bacterial and wound healing activity of the fruits of <i>Amorpha fruticosa l.</i>

Qu¹,

Diao²,

Zhang³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

show abstract

“…Fruticin showed an inhibitory effect on rat heart phosphodiesterase. Its activity was found to be greater than the inhibitory activity of potent flavonoids such as luteolin, kaempferol, quercetin (Petkov et al, 1983). Hypercoagulability in rabbits with experimentally induced atherosclerosis was inhibited by oral administration of fruticin (Aǐzikov et al, 1984).…”

Section: Fruticin Contentmentioning

confidence: 99%

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

et al. 2020

View full text Add to dashboard Cite

Fruticin (amorphin) is a constituent of the fruit of the false indigo-bush (Amorpha fruticosa L.), which belongs to the class of rotenoid glycosides, and shows several interesting pharmacological activities. The aim of this study was to isolate and chemically characterize this natural product, as well as to determine the optimal period of the year for A. fruticosa fruits collection. Fruticin was obtained by re-crystallization of the precipitate that formed after partial evaporation of the extract, prepared by 3fold extraction of powdered plant material by chloroform - ethanol (1:1, v/v). The structure of the final product was determined by various techniques of instrumental analysis (NMR, UV and MS), and confirmed by comparing the obtained spectra with corresponding data in available literature. The content of fruticin in A. fruticosa fruit was determined by LC-DAD-MS, using the external standard method based on the constructed calibration curve. Limits of detection (LOD) and quantification (LOQ) were also determined. A substantial increase in fruticin content was observed during the ripening period (>50 %). It has also been established that the optimal time for fruit collection is mid-December. Obtained results indicate that the content of fruticin in the A. fruticosa fruit is highly dependable on the time of collection. Since the biosynthesis of secondary plant metabolites is influenced not only by the time of collection, but by numerous other factors as well, additional studies are needed to define, with greater certainty, the conditions that are necessary for design of prospective efficient and sustainable production process.

show abstract

“…Petkov et al(1981) and Nikaido et al(1982) have shown that flavonoids possess an inhibitory action on cyclic (c) AMP phosphodiesterase. Since inhibition of this enzyme is associated with an increase of CAMP, it has been suggested that this increase offers a basis for the explanation of the myolytic activity of flavonoids (Petkov et al 1983). On the other hand, it has been documented that phosphodiesterase, like many other enzyme systems, is regulated by the Ca2+-binding protein calmodulin (West 1982).…”

Section: M)mentioning

confidence: 99%

Effects of 3,3′-di-O-methylquercetin on guinea-pig isolated smooth muscle

Abdalla

Zarga

Afifi

et al. 1989

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

The effects of the flavone 3,3'-di-O-methylquercetin (DOMQ) have been examined and compared with those of quercetin, on guinea-pig isolated ileum, trachea, and main pulmonary artery (MPA). Except for transient contractions induced by low concentrations (10(-8)-3 x 10(-6) M), DOMQ and quercetin (up to 3 x 10(-4) M) caused reduction of the tone and the phasic contractions of the ileum. A23187 reversed the inhibitory effects of quercetin but not those of DOMQ. DOMQ and quercetin caused concentration-dependent relaxation of the trachea and the adrenaline-contracted MPA. DOMQ shifted to the right the concentration-effect curves induced by acetylcholine on the ileum and the trachea, and by adrenaline on MPA and those induced by CaCl2 on ileum, trachea and MPA. DOMQ also inhibited the contractions induced, in Ca2+-free EGTA-containing buffer, by histamine on ileum and by adrenaline on MPA. These observations suggest that DOMQ inhibits Ca2+ influx, Ca2+ release from intracellular stores and, more likely, Ca2+ binding to intracellular receptor proteins.

show abstract

Inhibition of Rat Heart Phosphodiesterase by some Rotenoids and Isoflavonoids

Cited by 17 publications

References 9 publications

Evaluation of anti-bacterial and wound healing activity of the fruits of <i>Amorpha fruticosa l.</i>

Evaluation of anti-bacterial and wound healing activity of the fruits of <i>Amorpha fruticosa l.</i>

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

Effects of 3,3′-di-O-methylquercetin on guinea-pig isolated smooth muscle

Contact Info

Product

Resources

About