INHIBITION OF RAT PITUITARY THYROTROPHIC, GONADOTROPHIC AND GROWTH HORMONE ACTIVITY WITH PITUITARY ANTISERUM¹

Neutralization of Activity of Circulating Gonadotrophic Hormones by Antiserum to Rat Pituitary

“…The inhibition of moderately strong hormonal activity by very small amounts of this potent antiserum suggests that the neutra¬ lization is effected by a specific antigen-antibody reaction. Evidence has been pre¬ viously presented that this inhibitory effect is probably not due to any non-specific toxic effect of the antiserum (Hayashida et al 1961). The likelihood of such a non¬ specific effect was considerably reduced by the prior absorption of the antiserum with homologous (rat) serum proteins, red blood cells and concentrated liver homogenate (Hayashida et al 1961).…”

“…Evidence has been pre¬ viously presented that this inhibitory effect is probably not due to any non-specific toxic effect of the antiserum (Hayashida et al 1961). The likelihood of such a non¬ specific effect was considerably reduced by the prior absorption of the antiserum with homologous (rat) serum proteins, red blood cells and concentrated liver homogenate (Hayashida et al 1961). In most studies of a similar nature in which the antiserum has been prepared against crude pituitary extracts, the control of non-specific or toxic factors has not received adequate attention.…”

“…It has been shown that a rabbit antiserum can be produced which contains anti¬ bodies to rat pituitary gonadotrophic, thyrotrophic, and growth hormones (Haya¬ shida, Rankin, McClelland & Contopoulos, 1960;Hayashida, Rankin, McClelland & Contopoulos, 1961). This antiserum, administered simultaneously with rat pituitary extract, neutralizes hormonal effects on target organs (testes, thyroid glands, tibial epiphysial plate) of recipient hypophysectomized rats (Hayashida et al 1961).…”

“…This antiserum, administered simultaneously with rat pituitary extract, neutralizes hormonal effects on target organs (testes, thyroid glands, tibial epiphysial plate) of recipient hypophysectomized rats (Hayashida et al 1961).…”

Inhibition of Spermiogenesis, Prostate and Seminal Vesicle Development in Normal Animals With Anti-Gonadotrophic Hormone Serum

“…It was observed that the anti-ICSH serum contained very large amounts of antibodies to sheep serum proteins. Interestingly, the amount of A/S protein anti¬ bodies present in the A/S was roughly about as much as present in A/S from rabbits immunized with crude extracts of rat anterior pituitary glands (Hayashida, Rankin, McClelland & Contopoulos, 1961). However, after adequate absorption, no reaction of the anti-ICSH serum could be demonstrated when allowing it to interact simul¬ taneously with 0-25 ml.…”