Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish

Lu, Fugang; Bai, Chenglian; Chen, Yuanhong; Dai, Jun; Xiang, Yang; Ji, Xiang‐Shan; Huang, Changjiang; Dong, Qiaoxiang

doi:10.1016/j.cryobiol.2014.09.005

Cited by 78 publications

(51 citation statements)

References 41 publications

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“…Recently, a research group observed similar effects of UCP2 expression in the spermatozoa of zebrafish and yellow catfish [22,23]. It was found that a UCP2 promoter significantly improved the viability of post-thaw spermatozoa, accompanied by the decreased levels of ROS and MDA [23]. The UCP2 expression in the zebrafish spermatozoa can be down-regulated by Genipin [30] consistent with our observation in humanspermatozoa, and furthermore, could be up-regulated by acute cold exposure [30].…”

Section: Discussionsupporting

confidence: 77%

“…In this study, the results demonstrate that UCP2 plays a pivotal rolein ROS scavenging in human spermatozoa, and thus affects spermatozoa motility. Recently, a research group observed similar effects of UCP2 expression in the spermatozoa of zebrafish and yellow catfish [22,23]. It was found that a UCP2 promoter significantly improved the viability of post-thaw spermatozoa, accompanied by the decreased levels of ROS and MDA [23].…”

Section: Discussionmentioning

confidence: 73%

“…The concentrations of Genipin used in this study are based on a published study [23]. Interestingly, as shown in Fig.…”

Section: Effect Of the Ucp2-specific Inhibitor On Spermatozoa Motilitmentioning

confidence: 99%

“…However, the function of UCP2 in male reproduction remains unclear. Recently, a research group found expression of UCP2 in the spermatozoa of zebrafish and yellow catfish and revealed the close relevance between the expression of UCP2 and spermatozoa ROS level, degree of oxidative damage, and post-thaw viability [22,23]. In human spermatozoa, the transcription of UCP2-encoding gene has been observed by quantifying mRNA,however, the UCP2 protein level was not further measured in the same study [24].…”

Section: Introductionmentioning

confidence: 99%

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UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Wang

Hua

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: To demonstrate the function of uncoupling protein 2 (UCP2) in the regulation of human spermatozoa motility. Methods: Semen samples were collected from donors with either normal spermatozoa motility (normospermia [NS]) or poor spermatozoa motility (asthenospermia [AS]). UCP2 protein in spermatozoawas quantified by Western blotting. The level of mitochondrial reactive oxygen species (mROS) was evaluated by MitoSOX Red. The activity of mitochondrial membrane potential (MMP) in spermatozoa was evaluated by a JC-1 assay and the ATP level was monitored by a luciferin-luciferase assay. Results: UCP2 was expressed in both NS and AS groups, with the former exhibiting a higher level than the latter. Immunofluorescence analysis shows that UCP2 is mainly located at the mid-region of human spermatozoa. The inhibition of UCP2 by a highly selective inhibitor, Genipin, results in not only impaired spermatozoa mobility (P<.05) but also an elevated level of mROS (P<.05), suggesting that UCP2 is involved in the maintenance of the spermatozoa mobility, which probably is achieved by promoting mROS elimination. Furthermore, H₂O₂ treatment of spermatozoa increases the mROS level coupled with the loss of spermatozoa mobility. Unexpectedly, this treatment also has a positive impact on the expression of UCP2 within a certain range of supplemental H₂O₂, indicating the moderate mROS level possibly serves as a feedback signal to stimulate the expression of UCP2. Finally, the treatment of spermatozoa by an ROS scavenger, N-acetyl-l-cysteine (NAC),decreases the level of mROS and increases the curvilinear velocity (VCL) of spermatozoa, but the UCP2 level is not affected. Conclusion: These results suggest an UCP2–mROS–motility regulatory system exists for maintaining spermatozoa mobility in humans. In such a system, UCP2 fulfills its function by promoting mROS elimination, and slightly over-produced mROS in turn serves as a signal to stimulates the expression of UCP2. This regulatory system represents a new potential target for the discovery of novel pharmaceuticals for the treatment of patients with low spermatozoa motility.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 73%

“…The concentrations of Genipin used in this study are based on a published study [23]. Interestingly, as shown in Fig.…”

Section: Effect Of the Ucp2-specific Inhibitor On Spermatozoa Motilitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Wang

Hua

Huang

et al. 2018

Cell Physiol Biochem

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“…ROS Mitochondrial uncoupling separates oxidative phosphorylation from adenosine triphosphate 35 (ATP) synthase with energy dissipated as heat, and incomplete or mild mitochondrial uncoupling 36 has been shown to reduce oxidative damage [9]. For example, uncoupling induced by the 37 chemical protonophore 2,4-dinitrophenol (DNP) has been shown to be beneficial for in vitro 38 culture of porcine and bovine embryos [31; 45], and for sperm cryopreservation in rhesus 39 macaques [14] and yellow catfish [19]. Physiological uncoupling is mediated by protein 40 complexes embedded in the mitochondrial inner membrane, most notably the uncoupling 41 proteins (UCPs) [3; 42].…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish

et al. 2015

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The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

Aktar,

Toker,

Koca

et al. 2024

Veterinary Medicine & Sci

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ObjectivesThis study aimed to examine the effects of supplementation of vitamin D to the egg‐yolk extender on characteristics of frozen–thawed ram semen.MethodsSemen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.ResultsTotal motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05).ConclusionsIn the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.

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Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish

Cited by 78 publications

References 41 publications

UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish

The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

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