Inhibition of s-nitrosation of reduced glutathione in aerobic solutions of nitric oxide by phosphate and other inorganic anions

DeMaster, Eugene G.; Quast, Barry J.; Mitchell, Robert A.

doi:10.1016/s0006-2952(96)00825-8

Cited by 17 publications

(13 citation statements)

References 16 publications

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“…Although the higher pH and high concentration of inorganic phosphate in the mitochondrial matrix do not favor S-nitrosation of proteins [63], and the matrix enzymes glutathione peroxidase [64] and thioredoxin reductase [65] accelerate the decomposition of S-nitrosothiols within the matrix, the inner mitochondrial membrane and intermembrane space appear to be preferred sites of protein S-nitrosation because S-nitrosation is favored by lower pH [2] and lipophilic membranous environments [66]. The S-nitrosation of a mitochondrial intermembrane protein, caspase-3 [62], and inner membrane embedded proteins, the complex I [67], might indicate a crucial role for mitochondria in the regulation of cell signaling by NO.…”

Section: No In Mitochondriamentioning

confidence: 96%

Mitochondrial nitric oxide synthase

Ghafourifar

Cadenas

2005

Trends in Pharmacological Sciences

379

279

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Section: No In Mitochondriamentioning

confidence: 96%

Mitochondrial nitric oxide synthase

Ghafourifar

Cadenas

2005

Trends in Pharmacological Sciences

379

279

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“…Tris buffer was used instead of Krebs buffer to avoid the interfering effect of phosphate anions on NO transfer reactions (DeMaster et al 1997). SOD was added to stabilise NO in solutions.…”

Section: Preparations Of Subcellular Fractions Of Urethral Smooth Musmentioning

confidence: 99%

Differential mechanisms of urethral smooth muscle relaxation by several NO donors and nitric oxide

Garcia-Pascual¹,

Costa²,

Labadía³

et al. 1999

Naunyn-Schmiedeberg's Archives of Pharmacology

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We have examined the mechanisms of action of a broad spectrum of nitric oxide (NO) donors, including several S-nitrosothiols, sodium nitroprusside (SNP) and nitroglycerine (GTN), in relation to their relaxant activity of urethral smooth muscle. For all the compounds examined, NO release (in solution and in the presence of urethral tissue), relaxation responses, elevations in cGMP levels and the effect of thiol modulators were evaluated and compared with the effect of NO itself. Whilst all NO donors, except GTN, released NO in solution due to photolysis or chemical catalysis, this release was not correlated with their relaxant activity in sheep urethral preparations, which were furthermore not affected by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (cPTIO; 0.3 mM). A substantial NO-generating activity was found for S-nitroso-L-cysteine (CysNO) and S-nitroso-N-acetyl-D,L-penicillamine (SNAP) in the presence of urethral cytosolic fractions, suggesting metabolic activation to NO in the cytosol of the target tissue. In contrast, NO generation from S-nitroso-N-acetyl-L-cysteine (N-ac-CysNO), S-nitrosoglutathione (GSNO) and SNP were reduced by the presence of urethral homogenate and/or subcellular fractions, suggesting direct NO transfer to tissue constituents. NO donors and NO gas induced dissimilar degrees of cGMP accumulation in urethral tissue, while they were essentially equipotent as urethral relaxants. Furthermore, 1H-[1,2,4] -oxadiazole-[4,3-a]-quinoxalin-1-one (ODQ; 10 microM) inhibited both relaxation and cGMP accumulations, but with different potency for the different compounds. Oxidation of sarcolemmal thiol groups with 5-5'-dithio-bis[2-nitrobenzoic acid] (DTNB; 0.5 mM) enhanced relaxations to GSNO, an effect that was reversed by dithiotreitol (DTT; 1 mM), suggesting a direct effect through nitrosylation/oxidation reactions at the cell membrane, while relaxations to NO and to all the other compounds were not affected by these treatments. Finally, photodegradation of SNP induced the formation of a stable intermediate that still evoked NO-cGMP-mediated relaxations. This indicates that the assumption that SNP is fully depleted of NO by exposure to light should be revised. It can be concluded that important differences exist in the mechanisms by which distinct NO donors relax urethral smooth muscle and they cannot be regarded simply as NO-releasing prodrugs.

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“…The percentage hydrolysis of PPi promoted by [Cotn 2 (aq)] 3+ and nitric oxide was found to be much less than those of NPP under similar conditions. We postulate that the nitrosated intermediates of PPi moieties hydrolyze via an intramolecular mechanism as advanced in the literature (Demaster et al 1997). The nitrosated PPi would decompose to nitrite and a meta-phosphate intermediate followed by rapid hydration of the latter back to pyrophosphate as shown below.…”

Section: Discussionmentioning

confidence: 85%

Nitric Oxide-Triggered Bis-Trimethylenediaminecobalt(III)-Mediated Hydrolysis of Nitrophenylphosphate/Sodium Pyrophosphate

Tafesse

Enemchukwu

2009

Water Air Soil Pollut

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The synergistic effects of cobalt trimethylenediamine and nitric oxide toward the hydrolysis of nitrophenylphosphate/sodium pyrophosphate for different reactant ratio were investigated. Sodium nitroprusside under irradiation by UV light at 254 nm was utilized as nitric oxide donor in solutions. An increased hydrolysis was observed for activated phosphate moieties (nitrophenylphosphate/cobalt pyrophosphate) compared to that of pyrophosphate. The direct interaction of nitric oxide with the phosphorous center is presumed to be the reason for enhanced hydrolysis in those reaction solutions. This study demonstrates the possible role of nitric oxide in decontamination reactions of poorly biodegradable phosphate esters in natural waters due to phototransformation.

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Inhibition of s-nitrosation of reduced glutathione in aerobic solutions of nitric oxide by phosphate and other inorganic anions

Cited by 17 publications

References 16 publications

Mitochondrial nitric oxide synthase

Mitochondrial nitric oxide synthase

Differential mechanisms of urethral smooth muscle relaxation by several NO donors and nitric oxide

Nitric Oxide-Triggered Bis-Trimethylenediaminecobalt(III)-Mediated Hydrolysis of Nitrophenylphosphate/Sodium Pyrophosphate

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