Inhibition of Listeria monocytogenes growth on vacuum packaged rainbow trout (Oncorhynchus mykiss) with carvacrol and eugenol

Abstract

The aim of this study was to evaluate the effects of carvacrol and eugenol, separately and in combination, on survival of Listeria monocytogenes, and sensory and microbiological characteristics in vacuum packaged Oncorhynchus mykiss during refrigerated storage (4 ± 1 °C) for 20 days. The control fish fillets were analyzed for microbial (total mesophilic and psychrotrophic bacteria and lactic acid bacteria) and sensory properties. Fish fillets treated with carvacrol, eugenol, and their combination displayed populations of L. monocytogenes significantly lower, by 1.35–2.84 log cfu/g, than the control fillets during the whole storage period. No significant differences between groups of fish fillets with different active compound(s) added were noted except at the end of the storage, when the number of L. monocytogenes was significantly lower in the fish fillets with eugenol added. Sensory analysis showed that fish fillets with eugenol added were the most acceptable to trained panelists. Practical applications Taking into account the increasing need for the production of safe fish and fish products and the fact that carvacrol and eugenol, which exhibited significant antilisterial effect, are generally recognized as safe they can find its practical application in fish industry. Furthermore, as these substances are major constituents of numerous essential oils they can be considered as natural preservatives and used in the organic production as a substitute with synthetic additives which can cause adverse effects.

Preservative Effects of Gelatin Active Coating Enriched with Eugenol Emulsion on Chinese Seabass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage

“…Eugenol could prolong the shelf life of seabass samples because of its remarkable inhibitory effect. Dimitrijević et al [30] also demonstrated that eugenol showed good antimicrobial activity on retarding TVC growth, similar results also shown towards the increase of H 2 S-producing bacteria, Pseudomonas spp., and psychrophile in the current research. H 2 S-producing bacteria is one of the specific spoilage organisms in seabass samples during cold storage [31].…”

The microbial safety of fish and fish products: Recent advances in understanding its significance, contamination sources, and control strategies

“…Listeria monocytogenes in seabream fillets packaged in 60 to 80% CO 2 and 40 to 20% N 2 was reduced by 1.5 log CFU/g after 16 days of storage at 0 °C, compared with an over 2 log CFU/g increase in air‐packaged fillets stored at 4 °C (Provincial et al., 2013). The L. monocytogenes population in vacuum‐packaged rainbow trout fillets decreased from 4.48 to 3.13 log CFU/g during storage at 4 °C for 20 days (Dimitrijevic et al., 2019).…”

Preservative Effects of Gelatin Active Coating Containing Eugenol and Higher CO2 Concentration Modified Atmosphere Packaging on Chinese Sea bass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage

“…The lower O 2 could inhibit the growth of aerobic spoilage bacteria and the decomposition of trimethylamine oxide (TMAO) into trimethylamine, which can form a strong antimicrobial barrier with high CO 2 . Furthermore, eugenol possesses "moderate-strong inhibitory" characteristics to inhibit the microbial growth to extend the shelf-life of fish during storage [28,29]. Compared with AP treated sea bass samples, the gelatin active coating containing eugenol and MAP treated samples significantly inhibited the microbial growth during superchilling storage at −0.9 • C. Marchese et al [30] reported that eugenol showed a good antimicrobial effect on Gram-negative bacteria.…”