Inhibition of sepsis‐induced acute kidney injury via the<scp>circITCH‐miR‐579‐3p‐ZEB2</scp>axis

Li, Qing; Wang, Tianyi; Wang, Ximin; Ge, Xinyu; Yang, Tao; Bai, Guang; Wang, Wei

doi:10.1002/tox.23682

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Various CirRNAs such as Circ_35953, Circ_HIPK3, Circ_0114427, CIRC_0114428, Circ_0001714, circ_0020339, Circ_RASGEF1B, Circ_0114428, circ-FANCA, CircNRIP1, circ0001818, and Circ_0001806 [ 16 , 23 , 25 – 34 ] have been implicated in LPS-induced renal tubular cell apoptosis. Conversely, others such as CircITCH, CircVMA21, CIRC_0008882, and Circ_0091702 [ 35 – 38 ] exhibit an opposing role. In our present study, we highlight the antiapoptotic role of mmu_Circ_26986, substantiated by both overexpression and knockdown experiments (Figs.…”

Section: Discussionmentioning

confidence: 99%

Discovery and verification of mmu_Circ_26986/hsa_Circ_0072463 as a potential biomarker and intervention target for sepsis-associated acute kidney injury

Peng,

Li,

Zhang

et al. 2024

Cell. Mol. Life Sci.

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Approximately 60% of septic patients developed acute kidney injury (AKI). The mortality rate of septic AKI (SA-AKI) is two to three times higher than that of septic without AKI (SA-non-AKI). The actual functions and mechanisms of CircRNAs in the pathophysiology of SA-AKI remain incompletely understood. Herein, we observed that the mmu_Circ_26986 could be induced by lipopolysaccharide (LPS) and cecum ligation and puncture (CLP) in BUMPT cell line and C57BL/6 mouse kidney, respectively. Functionally, mmu_Circ_26986 suppressed BUMPT cell apoptosis induced by LPS. Mechanistically, mmu_Circ_26986 sponged miRNA-29b-1-5p to upregulate the expression of PAK7. Overexpression of mmu_Circ_26986 ameliorated the progression of CLP-stimulated AKI through miRNA-29b-1-5p/PAK7 axis. In addition, we found that hsa_Circ_0072463, homologous to mmu_Circ_26986, suppressed LPS-induced HK-2 cells apoptosis via regulation of miRNA-29b-1-5p/PAK7 axis. Furthermore, sepsis patients with AKI had a higher level of hsa_Circ_0072463 compared to those without AKI. The sensitivity, specificity and AUC of hsa_Circ_0072463 were 78.8%, 87.9% and 0.866, respectively. Spearman's test indicated a noticeable positive correlation between plasma hsa_Circ_0072463 and serum creatinine in sepsis patients (r = 0.725). In summary, this study reveals that the mmu_Circ_26986/hsa_Circ_0072463 miRNA-29b-1-5p/PAK7 axis mediates septic AKI, and hsa_Circ_0072463 is a potential diagnostic marker for septic AKI.

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Section: Discussionmentioning

confidence: 99%

Discovery and verification of mmu_Circ_26986/hsa_Circ_0072463 as a potential biomarker and intervention target for sepsis-associated acute kidney injury

Peng,

Li,

Zhang

et al. 2024

Cell. Mol. Life Sci.

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“…Not only that, ZEB2 is also involved in the inflammatory response of cells and multiple metabolic processes such as cell formation, growth, differentiation, and apoptosis (47)(48)(49). Recent study confirmed that ZEB3 overexpression relieved LPS-induced HK-2 cell apoptosis and inflammatory cytokine production (50). Ding et al showed that ZEB2 could alleviate LPS-induced HK2 cell inflammation (51).…”

Section: Discussionmentioning

confidence: 99%

Circvma21-Related Pathway Alleviates Lipopolysaccharide-Induced Hk-2 Cell Injury

Li,

Fu,

Zeng

et al. 2024

Shock

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Background It is reported that circVMA21 has an inhibition effect on sepsis-induced acute kidney injury (AKI). Therefore, the underlying molecular mechanisms of circVMA21 in AKI are worthy of further investigation. Material and methods Lipopolysaccharide (LPS) was used to induce HK2 cell injury. CircVMA21, miR-337-3p and ZEB2 expression was tested by qRT-PCR. Cell growth was detected by CCK8 assay, EdU assay and flow cytometry. Protein levels were examined by western blot. The levels of inflammatory factors and oxidative stress markers were measured to evaluate cell inflammatory response and oxidative stress. RNA relationship as verified by dual-luciferase reporter assay, RIP assay and RNA pull-down assay. Results CircVMA21 had decreased expression in AKI patients. Overexpressed circVMA21 alleviated LPS-induced HK2 cell inflammation, apoptosis, and oxidative stress. Moreover, circVMA21 sponged miR-337-3p, and miR-337-3p targeted ZEB2. The inhibitory effect of circVMA21 on LPS-induced HK2 cell injury was reversed by miR-337-3p overexpression, and ZEB2 overexpression abolished the promotion effect of miR-337-3p on LPS-induced HK2 cell injury. Conclusions CircVMA21 could inhibit LPS-induced HK2 cell injury via miR-337-3p/ZEB2 axis.

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Circ_0059662 exerts a positive role in oxygen–glucose deprivation/reoxygenation-induced SK-N-SH cell injury

An,

Xu,

Yuan

et al. 2023

Exp Brain Res

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Inhibition of sepsis‐induced acute kidney injury via thecircITCH‐miR‐579‐3p‐ZEB2axis

Cited by 6 publications

References 41 publications

Discovery and verification of mmu_Circ_26986/hsa_Circ_0072463 as a potential biomarker and intervention target for sepsis-associated acute kidney injury

Discovery and verification of mmu_Circ_26986/hsa_Circ_0072463 as a potential biomarker and intervention target for sepsis-associated acute kidney injury

Circvma21-Related Pathway Alleviates Lipopolysaccharide-Induced Hk-2 Cell Injury

Circ_0059662 exerts a positive role in oxygen–glucose deprivation/reoxygenation-induced SK-N-SH cell injury

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