Inhibition of sexual stage-specific proteins results in reduced numbers of sexual stages and oocysts of Cystoisospora suis (Apicomplexa: Coccidia) in vitro

Feix, Anna Sophia; Cruz‐Bustos, Teresa; Ruttkowski, Bärbel; Joachim, Anja

doi:10.1016/j.ijpara.2022.09.006

Cited by 2 publications

(1 citation statement)

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“…Additionally, significant research has been carried out in Hammondia, Besnoitia, Neospora, and Sarcocystis (Cruz-Bustos et al, 2021;Ramakrishnan and Smith, 2021) as well as C. suis (Feix et al, 2020;Cruz-Bustos et al, 2022). However, understanding the metabolic requirements of the sexual stages is vital for targeted interventions at this life phase of the parasites (Ramakrishnan et al, 2019;Cruz-Bustos et al, 2022;Feix et al, 2022b), and so far, little is known about these due to often limited access to sexual stages of the mentioned parasites in vitro.…”

Section: Introductionmentioning

confidence: 99%

Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies

Cruz-Bustos,

Dolezal,

Feix

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionThe apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.MethodsTo advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.ResultsA total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.DiscussionThese findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.

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