Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3

Rattanachuay, Pattamarat; Kantachote, Duangporn; Tantirungkij, Manee; Nitoda, Teruhiko; Kanzaki, Hiroshi

doi:10.2225/vol13-issue1-fulltext-2

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…This finding is in accordance with previous reports showed Vibrio sp. can be inhibited by grampositive and gram-negative bacteria [35,36]. However, due to most of probiotic bacteria applying in aquaculture is gram-positive such as bacillus, lactobacillus,… [36], thus, the gram-positive bacteria are being selected for further study.…”

Section: Isolation and Identification Of Vibrio Parahaemolyticus Inhibiting Bacteriamentioning

confidence: 99%

Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition

Huy¹

2020

IJST

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Objective: The present study reported the isolation of lactic acid bacteria from the shrimp gastrointestinal tract and evaluation of its antagonistic activity against pathogenic bacteria. Methods/ statistical analysis: Lactic bacteria from the gastrointestinal tract of alive healthy shrimp were screened on sterilized MRS medium. Vibrio sp. inhibition by isolates was conducted by the agar-well diffusion assay. The isolates were identified by 16S rRNA sequencing. Antibacterial activity was investigated against 25 Vibrio sp. isolates, Staphylococcus aureus ATCC 25023, and Escherichia coli ATCC 85922. Hemolytic activity, salt, and antibiotic resistance were also evaluated. Findings: Total of 22 isolates exhibited Vibrio parahaemolyticus inhibition with antagonistic activities ranged from 220 AU/ml to 460 AU/ml. Sequence analysis of 16S rRNA indicated the 5 isolates belong to Weissella cibaria species, namely HN02, HN03, HN04, and HN06 AND HN07. Among these, W. cibaria HN05 had highest antagonistic activity and exhibited the broad inhibition spectrum against Vibrio sp. as well as E. coli ATCC 85922 but not S. aureus ATCC 25023. β-hemolytic and salt resistance assays showed that W. cibaria HN05 had no β-hemolytic activity and grew well in medium containing 5% of salt. Antibiotic resistance analysis indicated W. cibaria HN05 was suppressed by ampicillin, chloramphenicol, oxytetracycline hydrochloride, and chlortetracycline hydrochloride. Application/improvements: This is the first study on isolation and Article Type: Article

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Section: Isolation and Identification Of Vibrio Parahaemolyticus Inhibiting Bacteriamentioning

confidence: 99%

Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition

Huy¹

2020

IJST

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“…W3 has been proven to secrete secondary metabolites, particularly those of small molecular weight that inhibit vibrios i.e. V. harveyi (Rattanachuay et al 2010). The W3 isolate was grown in Frazier gelatin medium (Frazier and Rupp 1982) containing 2% NaCl under optimum growth conditions: 30°C on a shaker (Gallenkamp, orbital incubator), shaking speed 150 rev/min, time 18 h and the culture broth was used to extract antivibrio compounds.…”

Section: Bacteria Used and Culture Conditionsmentioning

confidence: 99%

“…Bacterial cells in 10% DMSO without addition of the EtOAc-W3 served as a negative control set, while bacterial cells in concentrations of nalidixic acid of 0.391-800 lg/ml served as a positive control set. This antibiotic was used as the positive control due to the report by Rattanachuay et al (2010) that shrimp pathogenic strains of V. harveyi were sensitive to nalidixic acid and in Thailand, this antibiotic is allowed for use in shrimp farming and hatcheries. The 96-well plate was incubated at 30°C for 24 h and the bacterial growth was measured as turbidity by a microplate reader at a wavelength of 660 nm.…”

Section: Shrimp Usedmentioning

confidence: 99%

“…Continued antibiotic use also leads to selection of antibioticresistant microbes and eventually these might transfer to humans as pathogens (Holmström et al 2003;Rahman et al 2009). Based on a list of the antibiotics that can be used in aquaculture, only nalidixic acid and norfoxazine can be used treat shrimp diseases effectively (Musa et al 2008;Rattanachuay et al 2010;Teo et al 2002). In addition, in Thailand sulphamethoxazole is extensively used in shrimp hatcheries, but this has only moderate ability to inhibit shrimp pathogens (Rattanachuay et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Our previous work also showed that one of most active antivibrio compounds of one of our isolates, Pseudomonas sp. W3, has a small molecular weight, is heat stable, pH resistant and mostly tolerant to a variety of enzymes such as lysozyme, proteolytic, lipolytic and amylolytic enzymes (Rattanachuay et al 2010). Hence, in the present study, the crude extracted compounds produced by strain W3 were investigated for their ability to control V. harveyi and also for their toxicity to shrimps for possible use in shrimp farming.…”

Section: Introductionmentioning

confidence: 99%

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Antivibrio compounds produced by Pseudomonas sp. W3: characterisation and assessment of their safety to shrimps

Rattanachuay

Kantachote

Tantirungkij

et al. 2010

World J Microbiol Biotechnol

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In order to explore compounds naturallly inhibitory to shrimp pathogenic vibrios, a culture filtrate of Pseudomonas sp. W3 at a pH of 2 was extracted with ethyl acetate (EtOAc) to produce 82.15 mg/l of a yellow-brown extract (EtOAc-W3) that had MIC values of 225-450 lg/ml against the growth of 18 shrimp pathogenic Vibrio harveyi strains. The MIC of EtOAc-W3 against the most pathogenic strain PSU 2015 was 450 lg/ml and this strain had the lowest LD 50 (50% lethal dose) to pacific white shrimp (Litopenaeus vannamei, PL 21). At this MIC value, EtOAc-W3 in artificial sea water (ASW) killed strain PSU 2015; however in natural sea water, only a partial growth inhibition was observed. The toxicity to pacific white shrimp and antivibrio activity of the EtOAc-W3 were investigated by conducting an experiment with 4 sets; native control (commercial ASW), EtOAc-W3 control (MIC/10, 45 lg/ml), challenge (inoculation 6.0 9 10 6 c.f.u./ml PSU 2015) and treatment (6.0 9 10 6 c.f.u./ml PSU 2015 ? 45 lg/ml EtOAc-W3). The same experiment was repeated by increasing the dose of EtOAc-W3 to 90 lg/ml (MIC/5). Both concentrations of EtOAc-W3 tested had no toxicity to postlarval shrimps. A significant decrease in shrimp mortality was observed over a 72 h period as approximately 80% of the shrimps died in each challenge set but only 63 and 23% died in the presence of 45 and 90 lg/ml EtOAc-W3. The major component of EtOAc-W3 was supposed to be 2-heptyl-4-quinolone (HHQ) by FAB-MS and 1 H-NMR analyses of the purified fraction.

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The purification and functional study of new compounds produced byEscherichia coli that influence the growth of sulfate reducing bacteria

Oyewole

Mitchell

Thresh

et al. 2020

Egyptian Journal of Basic and Applied Sciences

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The induction and inhibition of sulfate-reducing bacterial (SRB) growth are of academic and biotechnological interests. The purification and functional study of two compounds produced by Escherichia coli were reported in this research. One of the compounds induces the growth of SRB and was termed as SRB growth enhancer (SGE) and the other inhibits their growth and was referred to as SRB growth inhibitor (SGI). The E. coli was cultured in M9 medium (1:20) and grown at 37°C for 24 h under limited oxygen condition. The cultures were spun and the supernatant was filter-sterilized. The cell-free supernatant was purified by ion-exchange chromatography and size exclusion chromatography. The fractions obtained were tested for inhibitory and enhancing activities of serially diluted Desulfovibrio indonesiensis, D. vulgaris and D. alaskensis. The effect of SGE and SGI on the morphology of SRB cells and biofilm formation was investigated using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The structural analysis of purified SGE and SGI was carried out using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) spectrometry. SRB cultures in the presence of SGE showed two order of magnitude higher growths than untreated SRB cultures while with SGI they showed growth with two order of magnitude less. The SRB treated with the SGE improved the SRB growth while the inhibitor altered SRB morphology. The MALDI-TOF spectra in linear mode showed the compounds to be an oligomeric series with repeat~213 m/z unit between each peaks. The data revealed that the molecular mass of SGE is around 1700 Da while that of SGI is estimated as 2400 Da. The result of this study suggests that SGE can be used as a supplement in the media for rapid SRB detection while the SGI can be used for the prevention of SRB colonization.

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Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3

Cited by 9 publications

References 30 publications

Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition

Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition

Antivibrio compounds produced by Pseudomonas sp. W3: characterisation and assessment of their safety to shrimps

The purification and functional study of new compounds produced byEscherichia coli that influence the growth of sulfate reducing bacteria

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