Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†

Kinterová, Veronika; Kaňka, Jiří; Petruskova, Veronika; Toralova, Tereza

doi:10.1093/biolre/ioy254

Cited by 25 publications

(25 citation statements)

References 47 publications

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“…Oocytes were collected from ovaries of freshly sacrificed 12 months old animals. Porcine and bovine oocytes were obtained from the slaughterhouse material as described previously ( 38 , 39 ).…”

Section: Methodsmentioning

confidence: 99%

“…Injected mouse oocytes were cultured in M16 media (Merck) supplemented with IBMX in 5% CO 2 at 37°C for 20 h. Bovine oocytes were cultured in MPM media (prepared in house ( 39 )) containing 0.1 mM milrinone (Sigma) without a paraffin overlay in a humidified atmosphere at 39°C with 5% CO 2 for 20 h. Pig oocytes were cultured in M-199 MEDIUM (Gibco) supplemented with 1 mM dbcAMP, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 5.5 mM Hepes, antibiotics and 5% foetal calf serum (Sigma). Injected oocytes were incubated at 38.5°C in a humidified atmosphere of 5% CO 2 for 20 h.…”

Section: Methodsmentioning

confidence: 99%

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MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

Kataruka

Modrák

Kinterová

et al. 2020

Nucleic Acids Research

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Abstract MicroRNAs (miRNAs) are ubiquitous small RNAs guiding post-transcriptional gene repression in countless biological processes. However, the miRNA pathway in mouse oocytes appears inactive and dispensable for development. We propose that marginalization of the miRNA pathway activity stems from the constraints and adaptations of RNA metabolism elicited by the diluting effects of oocyte growth. We report that miRNAs do not accumulate like mRNAs during the oocyte growth because miRNA turnover has not adapted to it. The most abundant miRNAs total tens of thousands of molecules in growing (∅ 40 μm) and fully grown (∅ 80 μm) oocytes, a number similar to that observed in much smaller fibroblasts. The lack of miRNA accumulation results in a 100-fold lower miRNA concentration in fully grown oocytes than in somatic cells. This brings a knock-down-like effect, where diluted miRNAs engage targets but are not abundant enough for significant repression. Low-miRNA concentrations were observed in rat, hamster, porcine and bovine oocytes, arguing that miRNA inactivity is not mouse-specific but a common mammalian oocyte feature. Injection of 250,000 miRNA molecules was sufficient to restore reporter repression in mouse and porcine oocytes, suggesting that miRNA inactivity comes from low-miRNA abundance and not from some suppressor of the pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

Kataruka

Modrák

Kinterová

et al. 2020

Nucleic Acids Research

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“…The SCF complex consists of S-phase kinase-related protein 1 (SKP1), the backbone protein Cullin1, and a variable F-box protein (Cardozo and Pagano, 2004). Moreover, a recent research suggested that SCF ligases were necessary for oocytes maturation, expansion of cumulus cell, and fertilization (Kinterova et al, 2019). F-box proteins are a class of proteins that contain the F-box motif, which constitute the key component of the SCF and mainly mediate substrate recognition and substrate recruitment (Kipreos and Pagano, 2000).…”

Section: Introductionmentioning

confidence: 99%

FBXO34 Regulates the G2/M Transition and Anaphase Entry in Meiotic Oocytes

Zhao

Sun

et al. 2021

Front. Cell Dev. Biol.

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There are two important events in oocyte meiotic maturation, the G2/M transition and metaphase I progression. Thousands of proteins participate in regulating oocyte maturation, which highlights the importance of the ubiquitin proteasome system (UPS) in regulating protein synthesis and degradation. Skp1–Cullin–F-box (SCF) complexes, as the best characterized ubiquitin E3 ligases in the UPS, specifically recognize their substrates. F-box proteins, as the variable adaptors of SCF, can bind substrates specifically. Little is known about the functions of the F-box proteins in oocyte maturation. In this study, we found that depletion of FBXO34, an F-box protein, led to failure of oocyte meiotic resumption due to a low activity of MPF, and this phenotype could be rescued by exogenous overexpression of CCNB1. Strikingly, overexpression of FBXO34 promoted germinal vesicle breakdown (GVBD), but caused continuous activation of spindle assembly checkpoint (SAC) and MI arrest of oocytes. Here, we demonstrated that FBXO34 regulated both the G2/M transition and anaphase entry in meiotic oocytes.

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“…SKP1 is a key skeleton protein in Skp1-Cull-Fbox protein (SCF), which mediates the ubiquitination and degradation of different cyclins [77], thereby promoting the cell cycle [78]. SCF has also been found to be crucial for oocyte division and maturation [79], as well as the process of fertilization and implantation [80]. Here, the results imply that these target genes are probably related to sheep reproduction process, but the molecular mechanism through which they affect litter size is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profile of Key CircRNAs and MiRNAs in Oviduct that Affect Sheep Reproduction

Li¹,

He²,

Zhang³

et al. 2020

Preprint

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Background: CircRNA and miRNA, as classes of non-coding RNA, have been found to play pivotal roles in sheep reproduction. There are many reports of circRNA and miRNA in ovary and uterus, but few in oviduct. In this study, RNA-Seq was performed to analyze the expression profile of circRNA and miRNA in oviduct between follicular phase and luteal phase in FecBBB (MM) and FecB++ (WW) sheep.Results: The result showed that 15 circRNAs were found differentially expressed between the follicular phase and luteal phase of FecBBB genotype (MF VS. ML), no circRNAs were found differentially expressed between the follicular phase and luteal phase of FecB++ genotype (WF VS. WL). 9 and 1 differentially expressed miRNAs were identified in MF VS. ML, and WF VS. WL, respectively. GO and KEGG analysis showed that the host genes of circRNAs in MF VS. ML were mainly involved in the Rap1 signaling pathway and PI3K-Akt signaling pathway. GO and KEGG analysis of miRNAs showed that the predicted target genes were mainly involved in the Insulin secretion and Rap1 signaling pathway in MF VS. ML. In WF VS. WL, the result showed that the predicted target genes were mainly involved in the TGF-β signaling pathway, Insulin secretion and Rap1 signaling pathway. In 3 comparison groups, GO and KEGG analysis indicated that a number of host genes and target genes (XPR1, LPAR3, SLC7A11, RAB3A, PLCB3, CREB3L4, LPAR1, LPAR2, FGF18, TACR3, BMP6, SMAD4, SKP1) were found to influence sheep reproduction. Conclusion: The results of the study will help to elucidate the regulatory mechanisms of circRNAs and miRNAs in sheep reproduction. And, this study will enrich the sheep circRNA and miRNA databases, which provide a basis for further research on sheep reproduction.

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Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†

Cited by 25 publications

References 47 publications

MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes

FBXO34 Regulates the G2/M Transition and Anaphase Entry in Meiotic Oocytes

Transcriptome Profile of Key CircRNAs and MiRNAs in Oviduct that Affect Sheep Reproduction

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