Inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus gasseri isolated from the vaginal tract of cattle

Otero, María Claudia; Nader‐Macías, María Elena Fátima

doi:10.1016/j.anireprosci.2005.11.004

Cited by 96 publications

(47 citation statements)

References 30 publications

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“…LAB have frequently been considered to be a factor that influences S. aureus growth by physically and chemically changing the environment. Several possibilities have been proposed to explain the inhibition of S. aureus by LAB, including the production of bacteriocins (2) and hydrogen peroxide (2,58), competition for nutrients (28), and needless to say, acidification (4,20,52), even if the impact of the latter mechanism has been questioned (12). Studies of the inhibition of S. aureus virulence expression by LAB, including the inhibition of SE production, are quite scarce.…”

mentioning

confidence: 99%

Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures

Even

Charlier

Nouaille

et al. 2009

Appl Environ Microbiol

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Staphylococcus aureus is responsible for numerous food poisonings due to the production of enterotoxins by strains contaminating foodstuffs, especially dairy products. Several parameters, including interaction with antagonistic flora such as Lactococcus lactis, a lactic acid bacterium widely used in the dairy industry, can modulate S. aureus proliferation and virulence expression. We developed a dedicated S. aureus microarray to investigate the effect of L. lactis on staphylococcal gene expression in mixed cultures. This microarray was used to establish the transcriptomic profile of S. aureus in mixed cultures with L. lactis in a chemically defined medium held at a constant pH (6.6). Under these conditions, L. lactis hardly affected S. aureus growth. The expression of most genes involved in the cellular machinery, carbohydrate and nitrogen metabolism, and stress responses was only slightly modulated: a short time lag in mixed compared to pure cultures was observed. Interestingly, the induction of several virulence factors and regulators, including the agr locus, sarA, and some enterotoxins, was strongly affected. This work clearly underlines the complexity of L. lactis antagonistic potential for S. aureus and yields promising leads for investigations into nonantibiotic biocontrol of this major pathogen.

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mentioning

confidence: 99%

Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures

Even

Charlier

Nouaille

et al. 2009

Appl Environ Microbiol

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“…For analysis of hydrogen peroxide production (Juárez Tomás et al, 2004), cells were grown on PYG agar without resazurin and supplemented with 0.01 g horseradish peroxidase (type VI-A; Sigma) l 21 and 20 ml TMB (3,39,5,59-tetramethylbenzidine; Sigma) solution [25 mg TMB (ml ¢99.8 % ethanol) 21 ] l 21 . Hydrogen peroxide assays were incubated anaerobically for 7 days and then exposed to air under light-protected conditions at room temperature for 24 h and evaluated as described by Otero & Nader-Macías (2006) Production of short-chain volatile and non-volatile fatty acids and ammonium was analysed using anaerobic cultures in PYG broth and microaerobic cultures in URAS PYG broth. Meat extract was omitted for analysis of lactic acids.…”

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confidence: 99%

Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa

Kraatz

Wallace

Svensson

2011

International Journal of Systematic and Evolutionary Microbiology

123

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Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa Strain A2 is an anaerobic, variably Gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been described informally as a representative of 'Olsenella (basonym Atopobium) oviles'. Three phenotypically similar bacterial strains (lac15, lac16 and lac31 T ) were isolated in concert with Veillonella magna lac18 T from the mucosal jejunum of a pig. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strains A2, lac15, lac16 and lac31 T formed a genetically coherent group (100 % interstrain sequence similarity) within the bigeneric Olsenella-Atopobium branch of the family Coriobacteriaceae, class Actinobacteria. This group was most closely related to the type strains of the two recognized Olsenella species, namely Olsenella uli (sequence similarity of 96.85 %) and Olsenella profusa (sequence similarity of 97.20 %). The sequence similarity to the type strain of Atopobium minutum, the type species of the genus Atopobium, was 92.33 %. Unlike those of O. uli and O. profusa, outgrown colonies of strains A2, lac15, lac16 and lac31 T were opaque and greyish-white with an umbonate elevation on solid culture media. The four novel strains were characterized as being well-adapted and presumably indigenous to the gastrointestinal tract of homoeothermic vertebrates: they were mesophilic, microaerotolerant, neutrophilic and acidotolerant, bile-resistant, mucin-utilizing and markedly peptidolytic lactic acid bacteria. The results of DNA-DNA hybridizations, cellular fatty acid analysis and other differential phenotypic (physiological and biochemical) tests confirmed that strains A2, lac15, lac16 and lac31 T represent a novel species of the genus Olsenella. On the basis of the genotypic and phenotypic results, we therefore describe Olsenella umbonata sp. nov., with lac31 T (5CCUG 58604 T 5DSM 22620 T 5JCM 16156 T ) as the type strain and A2 (5CCUG 58212 5DSM 22619 5JCM 16157) as an additionally available reference strain. Also, based on our data, we propose emended descriptions of the genus Olsenella and the species Olsenella uli and Olsenella profusa.

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“…[13][14][15] The results of the present study indicate that at 25°C the inhibition of both S. aureus growth and sea expression is greater than that at 35°C in both Lactobacillus co-cultures. Sameshima et al and Gonzalez-Fandos et al indicated that S. aureus growth inhibition varied depending on the temperature.…”

Section: Discussionmentioning

confidence: 85%

“…18 Other previous reports also suggest that growth of S. aureus is generally inhibited to a greater degree at temperatures lower than 30°C when cultured with other organisms. 13,19 A number of propositions have been made to explain the mechanism of inhibition of S. aureus growth by LAB, including the production of bacteriocins and hydrogen peroxide, 15,19 competition for nutrients, and acidification.…”

Section: Discussionmentioning

confidence: 99%

The Inhibitory Effects of 2 Commercial Probiotic Strains on the Growth of Staphylococcus aureus and Gene Expression of Enterotoxin A

Parsaeimehr¹,

Azizkhani²,

Javan³

2017

Int J Enteric Pathog

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Inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus gasseri isolated from the vaginal tract of cattle

Cited by 96 publications

References 30 publications

Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures

Staphylococcus aureus Virulence Expression Is Impaired by Lactococcus lactis in Mixed Cultures

Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa

The Inhibitory Effects of 2 Commercial Probiotic Strains on the Growth of Staphylococcus aureus and Gene Expression of Enterotoxin A

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