Inhibition of Streptococcus mutans by a commercial yogurt drink

Abstract

Background/Purpose Studies have been focused on using probiotics to prevent caries. The lactobacillus probiotic bacteria in Yakult ® (LcY) has been shown to inhibit the growth or biofilm formation of Streptococcus mutans . However, sucrose in Yakult ® raised concerns. The purpose of this study was to determine effects of Yakult ® on the growth and adhesion of S. mutans . Materials and methods S. mutans was grown in serial diluted Yakult ® , filtered Yakult ® or 20% heated Yakult ® . S. mutans was co-cultured with LcY in media with or without diluted filtered Yakult ® , or in LcY grown in media with or without sugars. Colony forming units and pH values of bacterial cultures were determined. SYTO 9-stained adhered bacteria were observed. Results Yakult ® inhibited the growth of S. mutans . Filtering or heating Yakult ® reduced its inhibitory ability against S. mutans . The inhibitory effect of LcY against S. mutans was enhanced when cultured in the presence of 20% filtered Yakult ® . LcY cultured in sucrose media for 24 h inhibited the growth of S. mutans , but this effect was less evident when LcY was grown for 48 h. LcY grown in glucose or lactose media similarly reduced S. mutans growth. Culturing S. mutans with LcY grown in sucrose or glucose media reduced bacterial adhesion. However, co-culturing S. mutans with LcY grown in the lactose media did not decrease bacterial adhesion. Conclusion Yakult ® and its probiotic content may inhibit S. mutans growth and the effect may be moderated by the type of sugar added for LcY cultivation.

Manipulating the diseased oral microbiome: the power of probiotics and prebiotics

“…[ 44 , 53 , 55 ] These may be attributed to the antimicrobial effect of reuterin, but also co-aggregation with pathogenic bacteria as observed by Saha et al [ 53 ]. or competitive adhesion as demonstrated in lactobacilli contributing to Yakult’s gastrointestinal probiotics [ 147 , 148 ].…”

Antimicrobial potential of AH Plus supplemented with bismuth lipophilic nanoparticles on E. faecalis isolated from clinical isolates

“…Hereto, a 12-well plate, coated with (~35 mg) of AH Plus with or without BisBAL NPs were seeded with 1 × 10 6 cells/well in 100 µL of TSB (ATCC strain or a human isolated specimen) and incubated at 37°C for 48 h. The biofilms of E. faecalis strain 11420 and clinical isolates cultured in non-coated wells served as growth controls. After incubation, cells were stained with 20 µM SYTO 9 (Invitrogen, Carlsbad, CA, USA) [22][23][24] for 30 min at 37°C in the dark. After staining, the biofilm was washed with sterile water and observed at 485 nm by fluorescence microscopy (EVOS, Floyd, Thermo Fisher Scientific, MA, USA).…”