Inhibition of testosterone 5 -reductase activity and growth of accessory sex tissues in castrated male mice

Luttge, William G.; Jasper, Thomas W.; Sheets, Cindy S.; Gray, Heewon L.

doi:10.1530/jrf.0.0530045

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…Testosterone propionate, but not DHTP, stimulation of seminal vesicle and ventral prostate gland weight was prevented by simultaneous treatment with 17/3C. This finding is in agreement with similar reports of 17/3C action in CD-I mice (Luttge et al, 1977) and rats (Bradshaw,Awh,& Baum,Note 1). Moreover, deficits in T activation of mating behavior in castrated male rats produced by the administration of the antiandrogen flutamide were also associated with a decline in genital tissue weight (Gladue & Clemens, 1980;Gray, 1977).…”

Section: Discussionsupporting

confidence: 91%

“…It is possible that despite 5a-reductase inhibition there should still be T available to interact with androgen receptors and stimulate male sexual behavior. Luttge et al (1977) reported that 17/3C pretreatment of castrated CD-I mice receiving [ 3 H]T caused a reduction in [ 3 H]DHT and an increase in [ 3 H]T recovered from genital tissues. It is not yet known whether 17/3C has a similar effect on the concentration of T and DHT by cells in the central nervous system (CNS).…”

Section: Discussionmentioning

confidence: 99%

“…The 5a-reductase inhibitor 5-androsten-3-one-17/3-carboxylic acid (17/3C) was used. This compound has been shown to inhibit 5a-reduction of T to DHT in peripheral androgen target tissues of castrated CD-I mice (Luttge, Jasper, Gray, & Sheets, 1977). It was predicted that by preventing the conversion of T to DHT, TP activation of both male copulation and growth of accessory sex organs would be blocked.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Testosterone acts as a prohormone to stimulate male copulatory behavior in male deer mice (Peromyscus maniculatus bairdi).

Clemens

Pomerantz²

1982

Journal of Comparative and Physiological Psychology

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Testosterone acts as a prohormone to stimulate male copulatory behavior in male deer mice (Peromyscus maniculatus bairdi).

Clemens

Pomerantz²

1982

Journal of Comparative and Physiological Psychology

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A study of the enzyme activity in the seminal vesicles of castrated and hormone-replaced castrated mice

Rastogi¹,

Milone²,

Caliendo³

et al. 1979

Experientia

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Reduction of fertilizing capacity of epididymal spermatozoa by 5α-steroid reductase inhibitors

1981

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Summary. Antifertility effects on epididymal spermatozoa were tested by administration of 5a-reductase inhibitors to androgen substituted adult castrated male mice. The inhibitors depressed the in vitro fertilizing ability and in vitro blastocyst development in testosterone substituted castrated male mice.It has been demonstrated that in the epididymides of various species the maturation and storage of spermatozoa are androgen-dependent. After castration, the fertilizing capacity of epididymal spermatozoa can be maintained when testosterone (T), dihydrotestosterone (DHT) or 3a-androstanediol (3a-diol) are administered 2. Although the conversion from T to DHT is rather extensive and DHT is the major androgen in the nuclei of the epididymal cells 3, the conversion from T into DHT has never been proved to be essential for normal epididymal function. It has been reported that the concept of DHT as the growth promoting active androgen in most target organs can be demonstrated by the use of 4-androsten-3-one-17/?-carboxylic acid (17tiC). Growth of accessory sex tissues of T substituted castrated mice was reduced by 17/?C treatment, but not by 17/?C administered in combination with DHT 4. Different in vitro studies have shown that 17/?C is a potent inhibitor of T 5a-reductase 5-7. In the present study we have investigated the effects of the 5a-reductase inhibitor 17tiC and its methyl ester (17tiME) on the maintenance of the fertilizing capacity of epididymal spermatozoa in castrated mice injected with T. Besides 17/?C, 17flCME was injected since it can be argued that this anti-androgenic compound 5 might have a prolonged action in comparison to 17tiC. Materials and methods.Young adult male (C57BLxCBA)-F1 mice were castrated under tribromoethanol anaesthesia (each animal 10 rag). Starting on the day of castration the animals received daily s.c. injections of different steroids (Steraloids, Pawling, N.Y.), dissolved in 0.05 ml of sesame oil as outlined in the table. The concentrations administered were 5 lag testosterone propionate (TP), 10 lag dihydrotestosterone propionate (DHTP), 100 lag 17/3C and 100 lag 17tiME. On day 11, 17 h after the last injection, the animals were killed by cervical dislocation. One cauda epididymidis and ductus deferens were removed and placed in a sterile petri dish (Falcon) in 2 ml of an embryo culture medium 8. After cutting the tissues into pieces the petri dishes were placed in an incubator at 37 ~ and gassed with 5% CO 2 in air, allowing the spermatozoa to disperse into the medium, Each sperm suspension was tested with eggs of 2 superovulated (PMSG and hCG) 8-12-week-old female (C57BLxCBA)-F 1 mice. Recently ovulated oocytes were isolated from the oviducts 20 h after the hCG injection and released into 0.2 ml of medium under liquid paraffin oil (Baker). To start in vitro fertilization, a drop of 0.2 ml from the sperm suspensions was added to the cumulus masses. After 7 h oocytes were removed, washed 3 times, and checked for the presence of 2 pronuclei. For an additional 96 h the embryos w...

show abstract

Inhibition of testosterone 5 -reductase activity and growth of accessory sex tissues in castrated male mice

Cited by 7 publications

References 7 publications

Testosterone acts as a prohormone to stimulate male copulatory behavior in male deer mice (Peromyscus maniculatus bairdi).

Testosterone acts as a prohormone to stimulate male copulatory behavior in male deer mice (Peromyscus maniculatus bairdi).

A study of the enzyme activity in the seminal vesicles of castrated and hormone-replaced castrated mice

Reduction of fertilizing capacity of epididymal spermatozoa by 5α-steroid reductase inhibitors

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