Inhibition of the Anaphylactic Mechanism by Sympathomimetic Amines

Assem, E. S. K.; Schild, H. O.

doi:10.1159/000230439

Cited by 72 publications

(25 citation statements)

References 8 publications

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“…The effects of sympathomimetic amines on the HFC of human isolated leucocytes differed in some respects from their effects on histamine release from the same cells (Assem & Schild, 1971a).…”

Section: Discussionmentioning

confidence: 83%

“…In 10 M concentration, isoprenaline inhibited HFC more potently than did noradrenaline, whilst adrenaline had no definite inhibitory effect (Figure 1). In contrast, the potency of inhibition of histamine release was: isoprenaline > adrenaline > noradrenaline (Assem & Schild, 1969, 1971a. Salbutamol, a ,B-receptor agonist, generally recognized as being less potent than isoprenaline, also inhibited HFC less potently (Figure 2).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sympathomimetic Amines on the Histamine Forming Capacity of Human Leucocytes

Assem

1974

British J Pharmacology

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1The effects of at-and ,B-adrenoceptor stimulants on the histamine-forming capacity (HFC) of human isolated leucocytes have been studied, in vitro. 2 It was confirmed that antigen significantly stimulates the HFC of human leucocytes.3 ,B-Adrenoceptor stimulants, such as isoprenaline and salbutamol (10 -610 3 M) significantly inhibited the HFC of human leucocytes in the presence and absence of antigen. At concentrations lower than 10-6 M, this effect was not observed. In general the degree of inhibition of HFC by 3-adrenoceptor stimulants followed their potency as 3-adrenoceptor stimulants. 4 a-Adrenoceptor stimulants significantly stimulated leucocyte HFC; noradrenaline within a limited concentration of 1 -6 M, while stimulation was seen consistently with phenylephrine at concentrations of 10--10 M. Adrenaline, which stimulates both aX-and ,B-adrenoceptors, produced small inhibition, no effect, or a degree of stimulation. Phentolamine, an ca-adrenoceptor blocking agent, produced an effect opposite to that of the a-adrenoceptor stimulants, i.e. a significant inhibition of the HFC of human isolated leucocytes.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Effect of Sympathomimetic Amines on the Histamine Forming Capacity of Human Leucocytes

Assem

1974

British J Pharmacology

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“…These agents were also found to inhibit reagin-mediated reaction as well as disodium cromoglycate (DSCG). DSCG does inhibit reagin-mediated reaction with a fairly high specificity (6)(7)(8)(9)(10)(11)(12), however, inhibits not at all or only slightly non-reaginic antibody-mediated reactions (7)(8)(9)(10)(11)(12) and the mediator release by specific antigen from the leucocytes of allergic patient (13).…”

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confidence: 99%

A Comparative Study of the Anti-Allergic Effects of Disodium Baicalein 6-Phosphate (BPS) and Disodium Cromoglycate (DSCG)

Koda

Watanabe

Yanagihara

et al. 1977

Japanese Journal of Pharmacology

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Abstract-A comparative study was carried out on the effects of a soluble derivative of baicalein, disodium baicalein 6-phosphate (BPS) and disodium cromoglycate (DSCG) on the immediate type allergic reactions.BPS not only inhibited reaginic antibody mediated reactions including antigen-induced mediator release from monkey lung, homologous PCA in rats, and reaginic antibody-mediated degranulation of mast cell, but also non-reaginic antibody-mediated reactions such as mediator release from guinea pig lung sensitized with ovalbumin and that from human lung caused by anti IgE. The agent, however, did not affect the mediator release from lung of rats sensitized with dinitrophynylated ascaris extract plus Bordetella pertussis. On the other hand, DSCG showed characteristic properties as an inhibitor of reaginic antibody-mediated reaction.It is thus assumed that the functional site of reaginic antibody is well fixed with DSCG at a definite distance between the two-chromone-nuclei while that of IgG is readily fixed with the two molecules of baicalein or BPS.Baicalein and baicalin are flavones contained in the radix of Scutellaria baicalensis GEORGI (Woogon), which from ancient times has often been used in Chinese medicine as a remedy for allergic diseases and inflammation. We (1-5) have already reported the anti allergic activity of baicalein and its derivatives, and these substances also have a potent protective action against active and passive anaphylaxis. These agents were also found to inhibit reagin-mediated reaction as well as disodium cromoglycate (DSCG). DSCG does inhibit reagin-mediated reaction with a fairly high specificity (6-12), however, inhibits not at all or only slightly non-reaginic antibody-mediated reactions (7-12) and the mediator release by specific antigen from the leucocytes of allergic patient (13).From the viewpoint of chemical structures, baicalein is a monochromone derivative while DSCG is a symmetric dichromone derivative, as shown in Fig. 1

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“…Thus, reversible amine flux across the leukocyte membrane may not occur in this system. Anaphylactic histamine release is also inhibited by cyclic AMP-active agents, but the effect of these agents after the release reaction is in progress has not been studied (5,7). Therefore, the reversibility of amine flux in these systems remains to be investigated.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, allergic histamine release from leukocytes (2-4), immunological histamine release from human lung (5,6), and anaphylactic histamine release (7) are inhibited by increased intracellular levels of cyclic AMP. In the present studies, the role of cyclic AMP in leukocyte histamine release induced by human leukocyte lysates was investigated.…”

Section: Introductionmentioning

confidence: 99%

Histamine Release Induced by Human Leukocyte Lysates REABSORPTION OF PREVIOUSLY RELEASED HISTAMINE AFTER EXPOSURE TO CYCLIC AMP-ACTIVE AGENTS

Kelly¹,

White²

1973

J. Clin. Invest.

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A B S T R A C T The role of cyclic AMP in histamine release induced by human leukocyte lysates was investigated. Leukocytes were incubated with leukocyte lysates prepared by ultrasonic disruption, and histamine was determined fluorimetrically. Several cyclic AMPactive agents had a marked inhibitory effect on histamine release. Theophylline and isoproterenol produced 50% inhibition at concentrations of less than 10' M. Prostaglandin E1 and dibutyryl cyclic AMP inhibited release by 50% at 7 X 10' M and 6 X 10' M concentrations, respectively. Histamine, which has recently been shown to increase leukocyte cyclic AMP, had a pronounced inhibitory effect on lysate-induced histamine release, producing 50% inhibition at a concentration of only 2.5 X 1012 M.Leukocytes, incubated with leukocyte lysates, were sampled at various times and assayed for free histamine released into the incubation mixture supernates, and for bound histamine associated with the leukocyte buttons after centrifugation. Theophylline, prostaglandin E1 and dibutyryl cyclic AMP not only blocked histamine release, but also caused a progressive decrease in free histamine when added at any time up to 30 min after initiation of the release reaction. As the free histamine decreased after addition of the inhibitors, there was a corresponding increase in the bound histamine, suggesting that previously released histamine was reabsorbed by the leukocytes after exposure to cyclic AMPactive agents. Continued incubation of leukocytes in their own histamine after completion of the release reaction also resulted in reabsorption of the previously released histamine. Previous studies have indicated that cyclic AMP inhibits leukocyte histamine release. The A preliminary report of this work appeared in abstract form in 1972 Clin. Res. 20: 82.

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Inhibition of the Anaphylactic Mechanism by Sympathomimetic Amines

Cited by 72 publications

References 8 publications

Effect of Sympathomimetic Amines on the Histamine Forming Capacity of Human Leucocytes

Effect of Sympathomimetic Amines on the Histamine Forming Capacity of Human Leucocytes

A Comparative Study of the Anti-Allergic Effects of Disodium Baicalein 6-Phosphate (BPS) and Disodium Cromoglycate (DSCG)

Histamine Release Induced by Human Leukocyte Lysates REABSORPTION OF PREVIOUSLY RELEASED HISTAMINE AFTER EXPOSURE TO CYCLIC AMP-ACTIVE AGENTS

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