Inhibition of the in vitro activities of α‐amylase, α‐glucosidase and pancreatic lipase by yellow field pea (Pisum sativum L.) protein hydrolysates

Abstract

Summary The aim of this work was to produce yellow field pea protein‐derived peptides as inhibitors of α‐amylase, α‐glucosidase and pancreatic lipase activities. A pea protein concentrate was hydrolysed with alcalase, chymotrypsin, pepsin or trypsin and the hydrolysates separated into different fractions (<1, 1–3, 3–5, 5–10 kDa) by membrane ultrafiltration. Peptide sequence analysis showed that the alcalase hydrolysate had higher levels of di‐ and tripeptides when compared with the chymotrypsin, pepsin and trypsin hydrolysates. The peptide fractions inhibited α‐amylase and α‐glucosidase activities at levels that were similar to the unfractionated hydrolysates. The peptides were more active against α‐amylase (inhibition at μg level) than α‐glucosidase (mg level). In contrast, the fractionated peptides had reduced ability (IC50 >4.2 mg mL−1) when compared with the unfractionated hydrolysate (IC50 <4.2 mg mL−1) to inhibit lipase activity. Enzyme kinetic studies revealed that the peptides reduced α‐amylase activity through competitive inhibition. However, inhibition of α‐glucosidase activity was non‐competitive.

The nutraceutical value of grain legumes: characterisation of bioactives and antinutritionals related to diabesity management

“…α‐amylase and α‐glucosidase are key enzymes involved in the digestion of dietary starch, releasing oligosaccharides that can be further broken down to glucose, which is rapidly absorbed by the body. Therefore, the inhibition of α‐amylase/α‐glucosidase activities is regarded as an effective strategy for managing diabetes and weight gain (Awosika & Aluko, ).…”

Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

“…α-glucosidase is another enzyme that makes glucose available in the body by breaking down oligo- and disaccharides, converting them into absorbable monosaccharides. Therefore, in order to reduce serum glucose levels and manage related diseases, α-glucosidase inhibition has been proposed as a suitable approach [ 33 , 34 ]. This work showed that the vegetable extracts had weaker α-glucosidase inhibition than acarbose, the drug.…”

Characteristics of Biopeptides Released In Silico from Collagens Using Quantitative Parameters

“…Sequences of collagens (11), mainly collagen type I chains, were derived from the UniProt database of protein sequences (shortly, UniProt database) (providers: Swiss Institute of Bioinformatics, Lausanne, Switzerland and European Bioinformatics Institute, Hinxton, UK) [21] (http:/www.uniprot.org). They represented collagens derived from cow (Bos taurus; P02453), pig (Sus scrofa; A0A287BLD2), sheep (Ovis aries; W5P481), chicken (Gallus gallus; P02457), duck (Anas platyrhynchos platyrhynchos; A0A493T0N1), horse (Equus caballus; F6SSG3), salmon (Salmo salar; A0A1S3S6G4), rainbow trout (Oncorhynchus mykiss; O93484), goat (Capra hircus; A0A452FHU9), rabbit (Oryctolagus cuniculus; A0A5F9CPN0), and turkey (Meleagris gallopavo; G1NB83).…”

“…The biological function of individual peptides is also related to the inhibition of: angiotensin I-converting enzyme (ACE; EC 3.4.15.1), dipeptidyl peptidase IV (DPP-IV; EC 3.4.14.5), α-glucosidase (EC 3.2.1.20), α-amylase (EC 3.2.1.1) [10], and lipase (EC 3.1.1.3) [11]. The ACE-inhibiting activity of peptides contributes to the blood pressure reduction in humans and animals [12], while inhibitors of lipase are involved in combating obesity [11]. In contrast, other aforementioned enzyme inhibitors are involved in the regulation of blood sugar level (antidiabetic peptides) [10].…”