Inhibition of the I _h current in isolated peripheral nerve: A novel mode of peripheral antinociception?

Modular double sucrose gap apparatus for improved recording of compound action potentials from rat and mouse spinal cord white matter preparations

“…Typically, the inter-diffusion between compartments is minimized by applying grease around the preparation at points which traverse between the compartments; however, the quality of this insulation remains low (discussed in Mert, 2007), and the grease insulation is not compact enough to suit short WM strips. Rubber membranes with holes closely matching the preparations proved highly efficient in earlier sucrose gap experiments (Berger, 1963;Boev and Golenhofen, 1974;Cleemann and Suenson, 1984;Hoyle, 1987), including studies on most challenging C-fibers in peripheral nerves, which require higher stimulation intensities and shorter conduction distances (Jirounek et al, 1991;Gaumann et al, 1992;Robert and Jirounek, 1994;Erne-Brand et al, 1999;Dalle et al, 2001). In this paper we designed and tested a DSG apparatus with rubber membrane separation of the compartments that enhances the conditions for recording and analysis of CAPs and by virtue of the compactness of the compartments is ideally designed to accommodate relatively short preparations such as those derived from rat and mouse spinal cords.…”

Characteristics of HCN Channels and Their Participation in Neuropathic Pain

“…Previous work found that clonidine was able to strengthen and prolong the peripheral nerve blocking effect of local analgesics through inhibiting axonal I h [58,87]. Consistent with the role of I h in axonal conduction, Dalle et al reported that perineuronal administration of ZD7288 significantly alleviated the mechanical allodynia of partial sciatic nerve injury rats [84,88]. However, other studies indicated that ZD7288 was not able to block axonal conduction [27,51].…”

The efficacy and safety of clonidine and dexmedetomidine as an adjuvant in supraclavicular brachial plexus block for elective upper limb surgery

“…[10] The direct action of clonidine on the nerve can be explained on the basis of a study conducted by Dalle et al They proposed that clonidine, by enhancing activity -dependent hyperpolarization generated Data are given as mean±SD, P<0.001 is statistically highly significant by the Na/K pump during repetitive stimulation, increases the threshold for initiating the action potential causing slowing or blockage of conduction. [11] …”