Inhibition of the infectivity of influenza virus by tea polyphenols

Nakayama, Mikio; Suzuki, Kenji; Toda, Masako; Okubo, Sachie; Hara, Yukihiko; Shimamura, Tadakatsu

doi:10.1016/0166-3542(93)90008-7

Cited by 352 publications

(249 citation statements)

References 13 publications

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“…Several studies have demonstrated the effect of EGCG on the yield of infectious FluA and determined an ED 50 of 8 μmol/L; however, the mechanism of [22] . In the current study, a dramatic reduction in infectious progeny particles at 28 h post-infection, which coincided with the pattern of vRNA synthesis, was found in influenza-infected MDCK cells treated with EGCG.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Ling

Wei

et al. 2012

Acta Pharmacol Sin

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Aim: To study whether epigallocatechin gallate (EGCG), a green tea-derived polyphenol, exerted anti-influenza A virus activity in vitro and in vivo. Methods: Madin-Darby canine kidney (MDCK) cells were tested. The antiviral activity of EGCG in the cells was determined using hemagglutination assay and qPCR. Time of addition assay was performed to determine the kinetics of inhibition of influenza A by EGCG. The level of reactive oxygen species (ROS) were determined with confocal microscopy and flow cytometry. BALB/c mice were treated with EGCG (10, 20 or 40 mg·kg -1 ·d -1 , po) for 5 d. On the 3rd d of the treatment, the mice were infected with influenza A virus. Histopathological changes, lung index and virus titers in the lungs were determined. Results: Treatment of influenza A-infected MDCK cells with EGCG (1.25-100 nmol/L) inhibited influenza A replication in a concentration-dependent manner (the ED 50 value was 8.71±1.11 nmol/L). Treatment with EGCG (20 nmol/L) significantly suppressed the increased ROS level in MDCK cells following influenza A infection. In BALB/c mice infected with influenza virus, oral administration of EGCG (40 mg·kg -1 ·d -1 ) dramatically improved the survival rate, decreased the mean virus yields and mitigated viral pneumonia in the lungs, which was equivalent to oral administration of oseltamivir (40 mg·kg -1 ·d -1 ), a positive control drug. Conclusion:The results provide a molecular basis for development of EGCG as a novel and safe chemopreventive agent for influenza A infection.

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Section: Discussionmentioning

confidence: 99%

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Ling

Wei

et al. 2012

Acta Pharmacol Sin

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“…Numerous studies have demonstrated that plant polyphenols including the tea catechins (-) epigallocatechin gallate (EGCG) and theaflavin digallate [34], resveratrol (RV) [26], a polyphenol rich extract (CYSTUS052) [11], pomegranate polyphenol extract (PPE) [13] , oligonol [12], and Hydroxytyrosol (HT) [38], have potent antiviral activity against influenza virus, that are related to the nature of their interactions with viral particles. Additionally, indirect effects of some polyphenols, such as EGCG and strictinin, on host cells that might interfere with virus-cell membrane fusion have also been suggested [23,32]. Influenza virus budding and release are essential for the transmission of the virus and in the pathogenesis of disease.…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

et al. 2011

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Influenza A virus (IAV) infection is a major public health threat leading to significant morbidity and mortality. The emergence of drug-resistant virus strains highlights urgent needs to develop novel antiviral drugs with alternative modes of action. Pentagalloyl glucose (PGG), a natural occuring polyphenolic compound, possesses broad spectrum of biological activities. In this study, we found PGG has anti-influenza virus activity and investigated its possible mechanism(s) of action in vitro.

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“…Epigallocatechin gallate (EGCG), a major ingredient of green tea has been known to have a variety of physiological activities; anticarcinogenic, antioxidant, antiangiogenic, and antiviral activity (Nakayama et al, 1993;Yang and Wang, 1993;Katiyar and Mukhtar, 1997). In addition, EGCG was also reported to function as antidiabetic.…”

Section: Introductionmentioning

confidence: 99%

Epigallocatechin gallate, a constituent of green tea, suppresses cytokine-induced pancreatic β-cell damage

Han

2003

Exp Mol Med

107

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Cytokines produced by immune cells infiltrating pancreatic islets have been implicated as one of the important mediators of β-cell destruction in insulin-dependent diabetes mellitus. In this study, the protective effects of epigallocatechin gallate (EGCG) on cytokine-induced β-cell destruction were investigated. EGCG effectively protected IL-1β and IFN-γ-mediated cytotoxicity in insulinoma cell line (RINm5F). EGCG induced a significant reduction in IL-1β and IFN-γ-induced nitric oxide (NO) production and reduced levels of the inducible form of NO synthase (iNOS) mRNA and protein levels on RINm5F cells. The molecular mechanism by which EGCG inhibited iNOS gene expression appeared to involve the inhibition of NF-κB activation. These findings revealed EGCG as a possible therapeutic agent for the prevention of diabetes mellitus progression.

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Inhibition of the infectivity of influenza virus by tea polyphenols

Cited by 352 publications

References 13 publications

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

Epigallocatechin gallate, a constituent of green tea, suppresses cytokine-induced pancreatic β-cell damage

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