Inhibition of the intestinal sodium‐coupled glucose transporter 1 (SGLT1) by extracts and polyphenols from apple reduces postprandial blood glucose levels in mice and humans

Schulze, Christine; Bangert, Adina; Kottra, Gábor; Geillinger, Kerstin E.; Schwanck, Bettina; Vollert, Henning; Blaschek, W.; Daniel, Hannelore

doi:10.1002/mnfr.201400016

Cited by 121 publications

(103 citation statements)

References 62 publications

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“…Since quercetin is bioavailable and is filtered in kidneys, we tested whether the onion extract given to the human volunteers affected glucose disposal into urine after the OGTT. Such an effect was shown previously after ingestion of an apple extract rich in phlorizin (Schulze et al, 2014). Ingestion of the onion extract resulted however not in any changes in urinary glucose output assessed over 24 hours after the OGTT.…”

Section: Effects Of the Onion Extract On Glucose Homeostasissupporting

confidence: 78%

“…However, it is also conceivable that by this mode of administration a certain amount of Q4′glc already underwent hydrolysis with release of quercetin which hardly inhibits SGLT1. We recently reported in a similar study that the administration of an apple extract 30 min prior to an OGTT resulted in a significant reduction in the rise of blood glucose and plasma insulin concentrations in healthy volunteers (Schulze et al, 2014). This suggests that the lack of an effect here is specific for the onion extract and not dependent on study design.…”

Section: Effects Of the Onion Extract On Glucose Homeostasismentioning

confidence: 72%

“…The experimental procedure was carried out as described previously by Schulze et al (2014). Apparent kinetic constants Km and Imax (nA) and IC50 values were determined as described by Kottra and Daniel (2007).…”

Section: Electrophysiological Studies In Oocytes Expressing Hsglt1mentioning

confidence: 99%

“…Afterwards mice were anaesthetized with isoflurane (Baxter, Unterschleißheim, Germany) and killed by cervical dislocation. The experimental procedure was carried out as described previously by Schulze et al (2014).…”

Section: Glucose Uptake Studies Employing Everted Jejunal Ringsmentioning

confidence: 99%

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Extracts and flavonoids from onion inhibit the intestinal sodium-coupled glucose transporter 1 (SGLT1) in vitro but show no anti-hyperglycaemic effects in vivo in normoglycaemic mice and human volunteers

Schulze¹,

Bangert²,

Schwanck

et al. 2015

Journal of Functional Foods

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Section: Effects Of the Onion Extract On Glucose Homeostasissupporting

confidence: 78%

Section: Effects Of the Onion Extract On Glucose Homeostasismentioning

confidence: 72%

Section: Electrophysiological Studies In Oocytes Expressing Hsglt1mentioning

confidence: 99%

Section: Glucose Uptake Studies Employing Everted Jejunal Ringsmentioning

confidence: 99%

See 2 more Smart Citations

Extracts and flavonoids from onion inhibit the intestinal sodium-coupled glucose transporter 1 (SGLT1) in vitro but show no anti-hyperglycaemic effects in vivo in normoglycaemic mice and human volunteers

Schulze¹,

Bangert²,

Schwanck

et al. 2015

Journal of Functional Foods

Self Cite

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“…The inhibitory activity of tea polyphenols against starch digestion in vivo is supposed to be caused by the inhibition of both starch-hydrolysing enzymes (α-amylase and α-glucosidase) and glucose transporters (GLUT2 and SGLT1) in the small intestine (Forester, Gu, & Lambert, 2012;C. Schulze, A. Bangert, G. Kottra, K. E. Geillinger, B. Schwanck, H. Vollert, et al, 2014).…”

Section: Teamentioning

confidence: 99%

Studying the interactions between tea polyphenols and α-amylase

Sun¹

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Dixon, Cornish-Bowden and Lineweaver-Burk plot analyses were applied to study the detailed kinetics of inhibition of porcine pancreatic α-amylase (PPA) by tea polyphenols.Fluorescence quenching (FQ), differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) were combined with the kinetics of inhibition to elucidate the mechanism of binding interactions of tea polyphenols and PPA. Then, the reciprocal of competitive inhibition constant (1/K ic ), fluorescence quenching constant (K FQ ) and binding constant (K itc ) obtained from these measurements were compared and correlated to analyse the relations between PPA inhibition and binding behaviour. The role of the galloyl moiety in binding of catechins and theaflavins with PPA was highlighted as well. In addition, the effects of three soluble polysaccharides (citrus pectin (CP), wheat arabinoxylan (WAX) and oat β-glucan (OBG)) on the inhibition of PPA by tea polyphenols were studied through PPA inhibition, IC 50 value, inhibition kinetics and fluorescence quenching methods. Furthermore, the effects of tea polyphenols on binding of PPA with normal maize starch granules were studied in terms of binding ratio, dissociation constant (K d ) and binding rate. Then, the association constant (1/K d ), 1/K ic and K FQ of tea polyphenols were correlated to analyse the effects of PPA inhibition on the binding of the enzyme with starch.The results show that green, oolong and black tea extracts, epigallocatechin gallate, theaflavin-3, 3'-digallate and tannic acid were competitive inhibitors of PPA, whereas epicatechin gallate, theaflavin-3'-gallate and theaflavin were mixed-type inhibitors with both competitive and uncompetitive inhibitory characteristics. Only catechins with a galloyl substituent at the 3-position showed measurable inhibition. K ic values were lower for theaflavins than catechins, with the lowest value for theaflavin-3, 3'-digallate. The lower K ic than the uncompetitive inhibition constant for the mixed-type inhibitors suggests that they bind more tightly with free PPA than with the PPA-starch complex. A 3 and/or 3'-galloyl moiety in catechin and theaflavin structures was consistently found to increase inhibition of PPA through enhanced association with the enzyme active site. A higher quenching effect of polyphenols corresponded to a stronger inhibitory activity against PPA. The red-shift of maximum emission wavelength of PPA bound with some polyphenols indicated a potential IV

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Does an apple a day keep away diseases? Evidence and mechanism of action

Zhang

Zeng

Zhang

et al. 2023

Food Science & Nutrition

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Apples and their products exemplify the recently reemphasized link between dietary fruit intake and the alleviation of human disease. Their consumption does indeed improve human health due to their high phytochemical content. To identify potentially relevant articles from clinical trials, some epidemiological studies and meta‐analyses, and in vitro and in vivo studies (cell cultures and animal models), PubMed was searched from January 1, 2012, to May 15, 2022. This review summarized the potential effects of apple and apple products (juices, puree, pomace, dried apples, extracts rich in apple bioactives and single apple bioactives) on health. Apples and apple products have protective effects against cardiovascular diseases, cancer, as well as mild cognitive impairment and promote hair growth, healing of burn wounds, improve the oral environment, prevent niacin‐induced skin flushing, promote the relief of UV‐induced skin pigmentation, and improve the symptoms of atopic dermatitis as well as cedar hay fever among others. These effects are associated with various mechanisms, such as vascular endothelial protection, blood lipids lowering, anti‐inflammatory, antioxidant, antiapoptotic, anti‐invasion, and antimetastatic effects. Meanwhile, it has provided an important reference for the application and development of medicine, nutrition, and other fields.

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Inhibition of the intestinal sodium‐coupled glucose transporter 1 (SGLT1) by extracts and polyphenols from apple reduces postprandial blood glucose levels in mice and humans

Abstract: Although the apple extract substantially decreased intestinal glucose absorption in all test systems, the finding that there are systemic effects that relate to inhibition of glucose transport processes beyond the intestine addresses safety issues that need further exploitation.

Cited by 121 publications

References 62 publications

Extracts and flavonoids from onion inhibit the intestinal sodium-coupled glucose transporter 1 (SGLT1) in vitro but show no anti-hyperglycaemic effects in vivo in normoglycaemic mice and human volunteers

Extracts and flavonoids from onion inhibit the intestinal sodium-coupled glucose transporter 1 (SGLT1) in vitro but show no anti-hyperglycaemic effects in vivo in normoglycaemic mice and human volunteers

Studying the interactions between tea polyphenols and α-amylase

Does an apple a day keep away diseases? Evidence and mechanism of action

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