Inhibition of the macrophage demethylase LSD1 reversesLeishmania amazonensis-induced transcriptomic changes and causes a decrease in parasite load

Abstract

Intracellular pathogens exploit host cell functions to favor their own survival. In recent years, the subversion of epigenetic regulation has emerged as a key microbial strategy to modify host cell gene expression and evade antimicrobial immune responses. Using the protozoan parasiteLeishmaniaas a model system, we have recently demonstrated that infection causes histone H3 hypomethylation, which is associated with the establishment of an anti-inflammatory phenotype, suggesting that host cell demethylases may play a role in the intracellular survival of these parasites. In this study, we employed a combination of pharmacological, RNA sequencing and interaction studies to investigate the role of the macrophage lysine demethylase LSD1 (KDM1a) inLeishmaniaintracellular infectionin vitro. Treatment of infected macrophages with two validated LSD1-specific inhibitors resulted in a significant reduction in parasite burden. We confirmed the impact of these inhibitors on LSD1 activity within macrophage nuclear extracts using anin vitrodemethylase assay and established their LSD1 target engagementin situby cellular thermal shift assay. RNA-seq analysis of infected and inhibitor-treated macrophages linked parasite killing to a partial reversion of infection-dependent expression changes, restoring the macrophage anti-microbial response and limiting cholesterol biosynthesis. While we ruled out any impact ofLeishmaniaon LSD1 expression or localization, we uncovered significant alterations in LSD1 complex formation within infected macrophages, involving unique interactions with host proteins as well as interactions withLeishmaniaproteins that appear to be secreted into the macrophage nucleus. Our study sheds important new light on the epigenetic mechanisms of macrophage immuno-metabolic subversion by intracellularLeishmaniaand identifies LSD1 as a potential candidate for host-directed, anti-leishmanial therapy.

MAPPING CHANGES OF MIRNA-MRNA NETWORKS INLEISHMANIA-INFECTEDMACROPHAGES PREDICTS REGULATORY MIRNA-TF LOOPS AS NOVEL TARGETS OF PARASITE IMMUNE SUBVERSION

“…Cholesterol biosynthesis represents one of the key biological processes induced during L. amazonensis infection believed to be essential for parasite development and vacuole membrane biogenesis (Osorio y Fortea et al . , Gutierrez Sanchez et al . 2023).…”

“…Recently, epigenetic subversion has emerged as a major strategy that these parasites use to escape host defenses (Kamhawi and Serafim 2020, Lecoeur et al . 2022, Gutierrez Sanchez et al . 2023).…”

“…Computational analysis of these data sets allowed us to map putative miRNA-mRNA regulatory networks that are predicted to control the observed inhibition of the pro-inflammatory TLR / NF-κB signalling pathway and increased cholesterol biosynthesis, both of which are prerequisites for intracellular parasite survival (Lecoeur et al . 2020, Gutierrez Sanchez et al . 2023).…”