Inhibition of the mitochondrial nicotinamide nucleotide transhydrogenase by dicyclohexylcarbodiimide and diethylpyrocarbonate.

NNT mediates redox-dependent pigmentation via a UVB- and MITF-independent mechanism

“…However, subsequent treatment of NNT overexpressing fish with 2,3 BD prevented the NNT OE-induced decrease in melanocytic pigmentation (Figure S5C). This finding is in line with previous publications confirming an inhibitory role 2,3BD and DCC on NNT enzyme activity (Phelps and Hatefi, 1981) (Moody and Reid, 1983). Next, we examined the status of NNT in human hyperpigmentation disorders including post inflammatory hyperpigmentation (PIH) and lentigo.…”

Inhibition of NADH-ubiquinone reductase by N,N'-dicyclohexylcarbodiimide and correlation of this inhibition with the occurrence of energy-coupling site 1 in various organisms

“…In the mitochondrial oxidative phosphorylation system, four energy-transducing enzymes (complexes III, IV, and V, and nicotinamide nucleotide transhydrogenase) have been shown to be inhibited by DCCD (Azzi et al, 1984;Hatefi, 1985;Phelps & Hatefi, 1981). The results presented in this paper demonstrate that complex I, the fifth energy-transducing enzyme of the mitochondrial oxidative phosphorylation system, is also inhibited by DCCD. In addition, it has been shown that DCCD inhibition of NADH-Q, reductase activity correlates with the presence of an energy-transducing site in this segment of the respiratory chain of various organisms.…”

The cutaneous redox system as a driver of skin pigmentation and skin cancer risk

“…However, subsequent treatment of NNT-overexpressing fish with 2,3BD prevented the NNT overexpression [OE]-induced decrease in melanocytic pigmentation (Figure S5C). This finding is in line with previous publications confirming an inhib-itory role 2,3BD and DCC on NNT enzyme activity (Phelps and Hatefi, 1981;Moody and Reid, 1983). Next we examined the status of NNT in human hyperpigmentation disorders, including postinflammatory hyperpigmentation (PIH) and lentigo.…”