Inhibition of the Soluble Form of Testis Adenylate Cyclase by Catechol Estrogens and Other Catechols

Braun, T.

doi:10.3181/00379727-194-43055

Cited by 22 publications

(22 citation statements)

References 17 publications

(31 reference statements)

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“…Curiously, norepinephrine induced sperm capacitation at 3.1 lM and inhibited sperm capacitation at 3.1 mM [20]. Other findings suggest that catecholamines inhibit the soluble form of testis adenylate cyclase and propose that the high-concentration effect may be mediated by cytosolic catechols [47].…”

Section: Discussionmentioning

confidence: 99%

The Presence and Function of Dopamine Type 2 Receptors in Boar Sperm: A Possible Role for Dopamine in Viability, Capacitation, and Modulation of Sperm Motility1

Ramírez-Reveco¹,

Castro²,

Angulo³

et al. 2009

Biology of Reproduction

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Several studies have shown that dopamine and other catecholamines are present in oviduct luminal fluid. We recently reported that dopamine type 2 receptors (DRD2) are present in a wide range of mammalian sperm, suggesting a role for dopaminergic signaling in events such as fertilization, capacitation, and sperm motility. In the present study, we used Western blot analysis to show that boar sperm express DRD2 and that their activation with dopamine (100 nM) has a positive effect on cell viability that can be correlated with AKT/PKB phosphorylation. Bromocriptine (100 nM) and dopamine (100 nM and 10 muM) increased tyrosine phosphorylation during the capacitation period. Immunofluorescence analysis indicated that DRD2 localization is dynamic and depends on the capacitation stage, colocalizing with tyrosine phosphorylated proteins in the acrosome and midpiece region of capacitated boar sperm. This association was confirmed by coimmunoprecipitation analysis. We also showed that bromocriptine (100 nM) and low-concentration dopamine (100 nM and 10 muM) increased total and progressive motility of sperm. However, high concentrations of dopamine (1 mM) decreased tyrosine phosphorylation and motility in in vitro sperm capacitation assays. This can be explained by the presence of the dopamine transporters (DAT, official symbol SLC6A3) in sperm, as demonstrated by Western blot analysis and immunocytochemistry. Taken together, our results support the idea that dopamine may have a fundamental role during sperm capacitation and motility in situ in the female upper reproductive tract.

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Section: Discussionmentioning

confidence: 99%

The Presence and Function of Dopamine Type 2 Receptors in Boar Sperm: A Possible Role for Dopamine in Viability, Capacitation, and Modulation of Sperm Motility1

Ramírez-Reveco¹,

Castro²,

Angulo³

et al. 2009

Biology of Reproduction

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“…This study has addressed this question employing the following compounds: 2-Hydroxyestradiol (2OHE2) -Catechol oestrogens are major products of oestrogen metabolism in the testes. These steroid metabolites are known to have disruptive effects on the germinal and epididymal epithelia as well as the male germ line, inhibiting soluble adenylyl cyclase, cross linking sperm chromatin and promoting ROS generation (Braun, 1990;Seegers et al, 1991a,b;Bennetts et al, 2008). Recent studies in breast tissue, where catechol oestrogens are thought to be involved in the aetiology of cancer (Okoh et al, 2011), have suggested that these compounds stimulate H 2 O 2 production from the mitochondria (Felty et al, 2005), making it a particularly good reagent for assessing methods purported to detect mitochondrial ROS production in spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid

et al. 2013

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SummaryOxidative stress is known to have a major impact on human sperm function and, as a result, there is a need to develop sensitive methods for measuring reactive oxygen species (ROS) generation by these cells. A variety of techniques have been developed for this purpose including chemiluminescence (luminol and lucigenin), flow cytometry (MitoSOX Red, dihydroethidium, 4,5‐diaminofluorescein diacetate and 2′,7′‐dichlorodihydrofluorescein diacetate) and spectrophotometry (nitroblue tetrazolium). The relative sensitivity of these assays and their comparative ability to detect ROS generated in different subcellular compartments of human spermatozoa, have not previously been investigated. To address this issue, we have compared the performance of these assays when ROS generation was triggered with a variety of reagents including 2‐hydroxyestradiol, menadione, 4‐hydroxynonenal and arachidonic acid. The results revealed that menadione predominantly induced release of ROS into the extracellular space where these metabolites could be readily detected by luminol‐peroxidase and, to a lesser extent, 2′,7′‐dichlorodihydrofluorescein. However, such sensitivity to extracellular ROS meant that these assays were particularly vulnerable to interference by leucocytes. The remaining reagents predominantly elicited ROS generation by the sperm mitochondria and could be optimally detected by MitoSOX Red and DHE. Examination of spontaneous ROS generation by defective human spermatozoa revealed that MitoSOX Red was the most effective indicator of oxidative stress, thereby emphasizing the general importance of mitochondrial dysregulation in the aetiology of defective sperm function.

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“…Mammals have nine tmAC genes (ADCY1-9), which differ in their tissue and developmental expression as well as in some of their regulatory properties (Cooper, 2003;Hanoune and Defer, 2001 (Braun, 1974;Braun, 1975;Braun, 1990;Braun, 1991;Braun and Dods, 1975). This enzyme was termed 'soluble adenylyl cyclase' (sAC) because its activity is preferentially found in the cytosolic fraction, although some activity is also found associated with membranes.…”

Section: Discovery Of Mammalian Sacmentioning

confidence: 99%

“…*Author for correspondence (mtresguerres@ucsd.edu) 1999; Chen et al, 2000), following on from earlier reports of Mn 2+ -stimulated cAMP activity in rat testis homogenates (Braun, 1974;Braun, 1975;Braun, 1990;Braun, 1991;Braun and Dods, 1975). This enzyme was termed 'soluble adenylyl cyclase' (sAC) because its activity is preferentially found in the cytosolic fraction, although some activity is also found associated with membranes.…”

Section: Reviewmentioning

confidence: 99%

Established and potential physiological roles of bicarbonate-sensing soluble adenylyl cyclase (sAC) in aquatic animals

Tresguerres

Barott

Barron

et al. 2014

Journal of Experimental Biology

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Soluble adenylyl cyclase (sAC) is a recently recognized source of the signaling molecule cyclic AMP (cAMP) that is genetically and biochemically distinct from the classic G-protein-regulated transmembrane adenylyl cyclases (tmACs). Mammalian sAC is distributed throughout the cytoplasm and it may be present in the nucleus and inside mitochondria. sAC activity is directly stimulated by HCO 3 -, and sAC has been confirmed to be a HCO 3 -sensor in a variety of mammalian cell types. In addition, sAC can functionally associate with carbonic anhydrases to act as a de facto sensor of pH and CO 2 . The two catalytic domains of sAC are related to HCO 3 --regulated adenylyl cyclases from cyanobacteria, suggesting the cAMP pathway is an evolutionarily conserved mechanism for sensing CO 2 levels and/or acid/base conditions. Reports of sAC in aquatic animals are still limited but are rapidly accumulating. In shark gills, sAC senses blood alkalosis and triggers compensatory H + absorption. In the intestine of bony fishes, sAC modulates NaCl and water absorption. And in sea urchin sperm, sAC may participate in the initiation of flagellar movement and in the acrosome reaction. Bioinformatics and RT-PCR results reveal that sAC orthologs are present in most animal phyla. This review summarizes the current knowledge on the physiological roles of sAC in aquatic animals and suggests additional functions in which sAC may be involved.KEY WORDS: V-ATPase, Acid/base, cAMP, Carbonic anhydrase, pH sensing, Proton pump IntroductionCyclic adenosine monophosphate (cAMP) is the signaling molecule of one of the most versatile and evolutionarily conserved signaling pathways. cAMP is produced by adenylyl cyclase enzymes that use ATP as substrate. There are six different classes of adenylyl cyclases distributed throughout Bacteria, Archaea and Eukarya; these classes are unrelated in sequence and structure but all produce cAMP as a result of convergent evolution (Linder and Schultz, 2008). All known eukaryotic adenylyl cyclases, including soluble adenylyl cyclase (sAC) and transmembrane adenylyl cyclase (tmAC) from animals, belong to Class III. Discovery of mammalian sACUntil recently, vertebrate animals were believed to have only one type of adenylyl cyclase, a family of hormone and G-proteinregulated tmACs. Mammals have nine tmAC genes (ADCY1-9), which differ in their tissue and developmental expression as well as in some of their regulatory properties (Cooper, 2003;Hanoune and Defer, 2001 (Braun, 1974;Braun, 1975;Braun, 1990;Braun, 1991;Braun and Dods, 1975). This enzyme was termed 'soluble adenylyl cyclase' (sAC) because its activity is preferentially found in the cytosolic fraction, although some activity is also found associated with membranes. Subsequent immunolocalization and biochemical studies found sAC in the cell cytoplasm and in organelles (Zippin et al., 2003;Zippin et al., 2004). The intracellular localization of sAC changed the assumption that cAMP is produced exclusively in the proximity of the cell membrane by tmACs. Pr...

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Inhibition of the Soluble Form of Testis Adenylate Cyclase by Catechol Estrogens and Other Catechols

Cited by 22 publications

References 17 publications

The Presence and Function of Dopamine Type 2 Receptors in Boar Sperm: A Possible Role for Dopamine in Viability, Capacitation, and Modulation of Sperm Motility1

The Presence and Function of Dopamine Type 2 Receptors in Boar Sperm: A Possible Role for Dopamine in Viability, Capacitation, and Modulation of Sperm Motility1

On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid

Established and potential physiological roles of bicarbonate-sensing soluble adenylyl cyclase (sAC) in aquatic animals

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