Inhibition of the toxic effects of Bothrops asper venom by pinostrobin, a flavanone isolated from Renealmia alpinia (Rottb.) MAAS

Gómez-Betancur, Isabel; Benjumea, Dora; Patiño, Arley Camilo; Jimenez, Nora; Osorio, Edison

doi:10.1016/j.jep.2014.08.002

Cited by 30 publications

(25 citation statements)

References 32 publications

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“…Some of these compounds are likely to form complexes with metals, such as Ca 2 + , but also their hydroxyl groups may form hydrogen bonds with amino acids at the catalytic site or at the point of attachment of divalent ions, thereby reducing the catalytic action of ion-dependent enzymes. [20] Snake venoms induce hemolytic activity mainly by the action of proteases such as metalloproteases and serine proteases, which degrade membrane proteins and modify the flow of ions and liquids through the membrane. In addition, phospholipases A 2 promote the breakdown of phospholipids that make up the cell membranes.…”

Section: Resultsmentioning

confidence: 99%

“…The lipase activity was determined according to Souza et al, [57] using 8 mmol L À 1 p-nitrophenyl palmitate in a 0.05 mmol L À 1 Tris-HCl buffer, pH 8.0, containing 0.5 % Triton X-100, as substrate. In the assay, the extracts and the lipase enzyme were incubated in a water bath, at 37°C, for four different time periods (10,20,30, and 40 minutes). After the incubation, the substrate was added.…”

Section: Lipase Activitymentioning

confidence: 99%

“…The trypsin activity was determined according to the methodology described by Erlanger et al [58] Thus, the aqueous and ethanolic extracts and the trypsin were incubated in a water bath at 37°C, for different time periods (10,20,30, and 40 minutes). Only then, the pbenzoyl-DL-arginine-p-nitroanilide (BAPNA) substrate, prepared in 0.05 mol L À 1 Tris, pH 8.2, was added.…”

Section: Trypsin Activitymentioning

confidence: 99%

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Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Braga

Abreu

Trento

et al. 2019

Chemistry & Biodiversity

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The aqueous and ethanolic extracts of Lippia sidoides Cham. were chemically characterized and tested for their action on enzymes involved in processes such as inflammation, blood coagulation, and digestion. Both extracts potentiated the activity of phospholipases A2 present in the venom of Bothrops atrox in 12 % and completely inhibited the hemolysis induced by B. jararacussu and B. moojeni venoms in the proportions between 1 : 0.5 and 1 : 5 (venom/extracts (w/w)). They inhibited the thrombolysis induced by B. moojeni (10 to 25 %), potentiated the thrombolysis induced by the Lachesis muta muta venom (30 to 80 %), prolonged the coagulation time induced by B. moojeni and L. muta muta venoms, and presented antigenotoxic action. Both extracts reduced the activity of α‐glycosidases, the aqueous extract inhibited lipases, and the ethanolic extract inhibited α‐amylases. The results demonstrate the modulatory action of the extracts on proteases, phospholipases, and digestive enzymes. In addition, the rich phenolic composition of these extracts highlights their potential for nutraceutical use.

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Section: Resultsmentioning

confidence: 99%

Section: Lipase Activitymentioning

confidence: 99%

Section: Trypsin Activitymentioning

confidence: 99%

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Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Braga

Abreu

Trento

et al. 2019

Chemistry & Biodiversity

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“…Also, several saponins were detected by NMR and TLC’s analysis, but were not isolated. In a previous study, it was demonstrated that 1 is responsible, in part, for the analgesic and anti-inflammatory activity of R. alpinia (Gomez-Betancur et al, 2014). Compounds 2–6 are reported for first time from this species.…”

Section: Resultsmentioning

confidence: 99%

“…The ethanolic extract of R. alpinia has been explored for its analgesic activity (Patiño et al, 2013). Later, it was found that the main component of the dichloromethane extract is the flavanone pinostrobin and it possessed inhibitory properties against Bothrops atrox-asper poison (Gomez-Betancur et al, 2014). in fact, three of the pure compounds were tested (all three major compounds), with Siegmund test, to assess activity in animal model on the peripheral nervous system, however, only statistically significant activity was obtained with the isolated major component, we mean with Pinostrobin (Gomez-Betancur et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive activity of extracts and secondary metabolites from wild growing and micropropagated plants of Renealmia alpinia

Gómez-Betancur

Cortés

Benjumea

et al. 2015

Journal of Ethnopharmacology

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Ethnopharmacological relevance Renealmia alpinia is native to the American continent and can be found from Mexico to Brazil, and in the Caribbean islands. It is known as “matandrea” in Colombia, and it has been commonly used in traditional medicine to treat painful diseases and ailments. Based on its traditional uses, it is of interest to evaluate the pharmacologic effects of this plant and its secondary metabolites. Materials and methods Methanol and aqueous extracts of wild and micropropagated R. alpinia (leaves) were obtained and chemically compared by High Performance Thin Layer Chromatography (HPTLC). The antinociceptive activity of these extracts was examined using an in vivo assay (Siegmund test). Additionally, the dichloromethane extract of R. alpinia was fractionated and pure compounds were isolated by chromatographic methods. The structure elucidation of isolated compounds was performed by NMR experiments and spectroscopic techniques and comparison with the literature data. Purified compounds were evaluated for their in vitro binding affinity for opioids and cannabinoids receptors. Results The dichloromethane extract of the plant’s aerial part afforded sinostrobin (1), naringenin 7,4′-dimethyl ether (2), 2′,6′-dihydroxy-4′-methoxychalcone (3), 4-methoxy-6-(2-phenylethenyl)-2H-pyran-2-one (4), naringenin 7-methyl ether (5) and 3,5-heptanediol, 1,7-diphenyl (6), which were isolated using chromatographic methods. Their chemical structures were established by physical and spectroscopic techniques. The antinociceptive effects observed in mice by extracts of wild and micropropagated plants were similar. The compounds isolated from R. alpinia do not show affinity to opioid or cannabinoid receptors. Conclusion Aqueous and methanol extracts of R. alpinia provide antinociceptive and analgesic effects in an in vivo model. These results contribute additional insight as to why this plant is traditionally used for pain management. Also, this is the first comprehensive report of a phytochemical study of R. alpinia.

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Protective effect of Dracontium dubium against Bothrops asper venom

Caro

Ocampo

Castro

et al. 2017

Biomedicine & Pharmacotherapy

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Inhibition of the toxic effects of Bothrops asper venom by pinostrobin, a flavanone isolated from Renealmia alpinia (Rottb.) MAAS

Cited by 30 publications

References 32 publications

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Antinociceptive activity of extracts and secondary metabolites from wild growing and micropropagated plants of Renealmia alpinia

Protective effect of Dracontium dubium against Bothrops asper venom

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