Inhibition of urease activity by humic acid extracted from sludge fermentation liquid

Xiang, Liu; Zhang, Min; Li, Zhengwen; Zhang, Chen; Wan, Chunli; Yi, Zhang; Lee, Dong Hoon

doi:10.1016/j.biortech.2019.121767

Cited by 42 publications

(28 citation statements)

References 36 publications

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“…we pipetted all of them into cuvettes sequentially with different pipettes and measured the activities at 630 nm wavelength. After finding a control value, we started the IC50 study, we found that their activities decreased by using different inhibitor concentrations, we determined that the activities decreased as the amount of inhibitor increased [14].…”

Section: P R E P R I N Tmentioning

confidence: 99%

Alpinetin: anti-human gastric cancer potential and urease inhibition activity <i>in vitro</i>

et al. 2021

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IntroductionAlpinetin is the bioactive component of a traditional Chinese medicine. This compound, one of the main constituents from the seeds of Alpinia katsumadai Hayata, belongs to flavonoids with its usefulness as anti-inflammatory, antibacterial, and other significant therapeutic activities of important potency and low systemic toxicity.Material and methodsThe molecular docking study as a complementary study was performed to provide additional data about the biological activities of alpinetin in the presence of urease. The docking calculations revealed that alpinetin with a docking score of -5.097 (kcal/mol) has an acceptable binding affinity to the enzyme, and because of various hydrophobic contacts and hydrogen bonds created by this chemical compound, alpinetin could be considered as an adequate inhibitor for urease. In the cellular and molecular part of the recent study, the treated cells with Alpinetin were assessed by MTT assay for 48h about the cytotoxicity and anti-human gastric carcinoma properties on normal (HUVEC) and gastric carcinoma cell lines i.e. SNU-1, Hs 746T, and KATO III.ResultsIn our study, inhibition result of Isoliquiritigenin on HMG-CoA reductase showed lower value IC50 = 21.86±1.44 µg / mL. The IC50 of Alpinetin were 426, 586, and 424 µg/mL against SNU-1, Hs 746T, and KATO III cell lines, respectively.ConclusionsThe viability of malignant gastric cell line reduced dose-dependently in the presence of Alpinetin. It seems that the anti-human gastric carcinoma effect of recent molecule is due to their antioxidant effects.

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Section: P R E P R I N Tmentioning

confidence: 99%

Alpinetin: anti-human gastric cancer potential and urease inhibition activity <i>in vitro</i>

et al. 2021

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“…Among known soil urease inhibitors, N-(n-Butyl)thiophosphoric triamide (NBPT) and hydroquinone are the most popular and potent compounds [ 17 ]. Many other compounds like acetohydroxamic acid (AHA) [ 18 ], humic acid [ 19 ], 1,4-benzoquinone and inorganic metal salts [ 16 ] have also been reported for their potential to reduce the activity of soil ureases. Natural plant materials have been extensively investigated to explore their urease inhibition potential but with an aim to get natural remedy against Helicobacter pylori infection in human stomach [ 9 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Soil urease inhibition by various plant extracts

2021

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Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be minimized by the inhibition of soil urease activity which has been explored using various potential chemical inhibitors. However, the soil urease activity inhibition potential of plant extracts is rarely explored to date. In the present study, extracts of 35 plant materials were taken and evaluated against jack bean urease. Eleven extracts, showing >50% jack bean urease inhibition, were selected and further investigated in 13 soils collected from various districts of Punjab, Pakistan. Interestingly, except Capsicum annum, Melia azedarach, Citrus reticulata and Quercus infectoria, the plant extracts showed urease inhibition activities in soils, the extent of which was lower as compared to that observed in jack bean urease though. Maximum urea hydrolysis inhibition (70%) was noted with Vachellia nilotica which was 40% more than that of hydroquinone (50%) followed by that of Eucalyptus camaldulensis (24%). The extracts of V. nilotica and E. camaldulensis were coated on urea and applied to soil in the next step. At 21st day, 239% and 116% more urea-N was recovered from soil treated with V. nilotica and E. camaldulensis extracts coated urea, respectively, as compared to uncoated urea. Conclusively, these results indicated that the coating of V. nilotica and E. camaldulensis extracts on urea prills prolonged urea persistence in soil owing to minimum urea hydrolysis, probably, the extracts of V. nilotica and E. camaldulensis showed their urease inhibition potential. The results of this study provide a base line for the identification of new soil urease inhibitor compounds from plant materials in future.

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“…The principle of these technologies is to promote the release of bound water, intracellular water and extracellular polymeric substances (EPS) into the water phase. However, the destruction of sludge cells and the release of organic matter increase the concentration of biochemical oxygen demand (BOD) and chemical oxygen demand (COD) in the sludge separation liquid, which not only increases the burden of subsequent processing, but also causes waste of resources Baeyens, 2003a, 2003b;Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…In this study, a simple, low-cost method for sludge dewatering and HA extraction was proposed. hydrothermal-alkali treatment was used to destroy the sludge ocs and cells to release the organic pollutants, and then HA was extracted from the decomposition liquid by using the characteristics of alkali dissolution and acid precipitation (Gu et al, 2019;Liu et al, 2019). Through single factor and response surface optimization (RSM) experiments, the optimal conditions were determined, and the mathematical models for accurately predicting the change of sludge water content and the extraction rate of HA were established.…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal-alkaline conversion of sewage sludge: Focus on optimization of process parameters and characterization of humic acid

Qiu

Xu²,

Wang³

et al. 2021

Preprint

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Sewage sludge (SS) dewatering is a key step in sludge disposal, which plays an important role in reducing sludge volume, facilitating transportation and subsequent treatment. In recent years, many studies have focused on sludge hydrothermal dewatering, while resource recovery during this process is less studied, especially humic substances. In this paper, a facile and low-cost hydrothermal-alkaline treatment for SS was proposed, which can be used for sludge dewatering and humic acid (HA) recycling at the same time. Response surface methodology (RSM) was used to determine the optimal conditions, and a mathematical model was established to accurately predict the change of sludge water content and the extraction rate of HA. Under the optimal conditions of 170 °C/42 min/0.05 (for hydrothermal temperature, hydrothermal time and mass ratio of KOH to wet sludge, respectively), the water content decreased to 46.7% and the extraction rate of HA (with a purity of 96.2%) was 89.1%. The improvement of the dewatering performance effectively facilitates the subsequent disposal of the sludge. The extraction of HA not only solves the problem that the sludge separation liquid needs further treatment, but also has potential economic value in agriculture, biological medicine, environment and other fields.

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Inhibition of urease activity by humic acid extracted from sludge fermentation liquid

Cited by 42 publications

References 36 publications

Alpinetin: anti-human gastric cancer potential and urease inhibition activity <i>in vitro</i>

Alpinetin: anti-human gastric cancer potential and urease inhibition activity <i>in vitro</i>

Soil urease inhibition by various plant extracts

Hydrothermal-alkaline conversion of sewage sludge: Focus on optimization of process parameters and characterization of humic acid

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