Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.

Augustine, Nimmy; Kumar, Praveen; Thomas, Sabu

doi:10.1007/s00203-010-0633-1

Cited by 53 publications

(36 citation statements)

References 18 publications

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“…4) (85), demonstrating for the first time the potential of quorum quenching in disease prevention. Following these initial reports, aiiA genes were identified in various other Bacillus species (56,60,(87)(88)(89), and heterologous expression of various aiiA alleles in numerous pathogenic bacteria, including P. aeruginosa and Burkholderia thailandensis, reduced AHL accumulation and altered QS-dependent behaviors (60,90). Additional studies have since provided further evidence of the utility of enzymatic quorum quenching for disease prevention and treatment in a variety of plant models using both transgenic plant species (91,92) and wild-type (WT) and engineered AiiA-expressing bacteria (88,89,(93)(94)(95)(96).…”

Section: Enzymatic Degradation and Inactivation Of Ahlsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

693

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Section: Enzymatic Degradation and Inactivation Of Ahlsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

693

556

View full text Add to dashboard Cite

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“…In the unicellular alga Chlamydomonas reinhardtii, expression of the codon-optimised AiiA altered the composition of the alga-associated microbiota (Rajamani et al 2011). When AiiA was expressed in V. cholera, it completely eradicated biofilm formation (Augustine et al 2010). Transgenic aiiA-expressing bacteria have also been used to disrupt industrial biofilms: the introduction of micro-encapsulated transgenic E. coli strongly reduced biofouling of membranes in wastewater treatment facilities over an extended period of time (Oh et al 2012).…”

Section: Enzymes That Degrade Biofilm-forming Qs Signalsmentioning

confidence: 99%

Mini-review: Inhibition of biofouling by marine microorganisms

2013

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Any natural or artificial substratum exposed to seawater is quickly fouled by marine microorganisms and later by macrofouling species. Microfouling organisms on the surface of a substratum form heterogenic biofilms, which are composed of multiple species of heterotrophic bacteria, cyanobacteria, diatoms, protozoa and fungi. Biofilms on artificial structures create serious problems for industries worldwide, with effects including an increase in drag force and metal corrosion as well as a reduction in heat transfer efficiency. Additionally, microorganisms produce chemical compounds that may induce or inhibit settlement and growth of other fouling organisms. Since the last review by the first author on inhibition of biofouling by marine microbes in 2006, significant progress has been made in the field. Several antimicrobial, antialgal and antilarval compounds have been isolated from heterotrophic marine bacteria, cyanobacteria and fungi. Some of these compounds have multiple bioactivities. Microorganisms are able to disrupt biofilms by inhibition of bacterial signalling and production of enzymes that degrade bacterial signals and polymers. Epibiotic microorganisms associated with marine algae and invertebrates have a high antifouling (AF) potential, which can be used to solve biofouling problems in industry. However, more information about the production of AF compounds by marine microorganisms in situ and their mechanisms of action needs to be obtained. This review focuses on the AF activity of marine heterotrophic bacteria, cyanobacteria and fungi and covers publications from 2006 up to the end of 2012.

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“…Impairing bacterial communication systems such as the quorum sensing can affect bacterial ability to form biofilm, as in the case of Bacillus cereus production of an AHL lactonase inhibiting Vibrio cholerae biofilm settlement [13]. Cugini et al (2007) also reported that C. albicans produces farnesol, a quorum sensing molecule, that inhibits the swarming mobility of P. aeruginosa , thereby enhancing its ability to form biofilm [14], [15].…”

Section: Introductionmentioning

confidence: 99%

Anti-Biofilm Activity: A Function of Klebsiella pneumoniae Capsular Polysaccharide

et al. 2014

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Competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. In this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producing bacterium, Klebsiella pneumoniae. Cell-free supernatant from K. pneumoniae planktonic cultures showed anti-biofilm effects on most Gram positive bacteria tested but also encompassed some Gram negative bacilli. The anti-biofilm non-bactericidal activity was further investigated on Staphylococcus epidermidis, by determining the biofilm biomass, microscopic observations and agglutination measurement through a magnetic bead-mediated agglutination test. Cell-free extracts from K. pneumoniae biofilm (supernatant and acellular matrix) also showed an influence, although to a lesser extend. Chemical analyses indicated that the active molecule was a high molecular weight polysaccharide composed of five monosaccharides: galactose, glucose, rhamnose, glucuronic acid and glucosamine and the main following sugar linkage residues [→2)-α-l-Rhap-(1→]; [→4)-α-l-Rhap-(1→]; [α-d-Galp-(1→]; [→2,3)-α-d-Galp-(1→]; [→3)-β-d-Galp-(1→] and, [→4)-β-d-GlcAp-(1→]. Characterization of this molecule indicated that this component was more likely capsular polysaccharide (CPS) and precoating of abiotic surfaces with CPS extracts from different serotypes impaired the bacteria-surface interactions. Thus the CPS of Klebsiella would exhibit a pleiotropic activity during biofilm formation, both stimulating the initial adhesion and maturation steps as previously described, but also repelling potential competitors.

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Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.

Cited by 53 publications

References 18 publications

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Mini-review: Inhibition of biofouling by marine microorganisms

Anti-Biofilm Activity: A Function of Klebsiella pneumoniae Capsular Polysaccharide

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