Inhibition on calcium oxalate crystallization and repair on injured renal epithelial cells of degraded soybean polysaccharide

Yao, Xiu-Qiong; Ouyang, Jing; Peng, Hua; Zhu, Wenyu; Chen, Hequn

doi:10.1016/j.carbpol.2012.05.056

Cited by 25 publications

(15 citation statements)

References 20 publications

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“…, which was significantly different from the multiple peaks of the COM crystals that appeared within the 3000 cm -1 to 3600 cm -1 region with multiple peaks. 16 The asymmetrical stretching vibration u as (COO -) of carbonyl appeared at 1647 cm -1 , whereas u s (COO -) appeared at 1328 cm…”

Section: Methodsmentioning

confidence: 97%

Interaction between submicron COD crystals and renal epithelial cells

Peng¹,

Ouyang²,

Yao³

et al. 2012

IJN

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Objectives: This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 ± 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation. Methods: Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. Scanning electron microscopy was used to observe Vero-COD adhesion. Inductively coupled plasma emission spectrometry was used to quantitatively measure the amount of adhered COD microcrystals. Nanoparticle size analyzer and laser scanning confocal microscopy were performed to measure the change in the zeta potential on the Vero cell surface and the change in osteopontin expression during the adhesion process, respectively. The level of cell injury was evaluated by measuring the changes in malonaldehyde content, and cell viability during the adhesion process. Results: The adhesion capacity of Vero cells in the injury group to COD microcrystals was obviously stronger than that of Vero cells in the control group. After adhesion to COD, cell viability dropped, both malonaldehyde content and cell surface zeta potential increased, and the fluorescence intensity of osteopontin decreased because the osteopontin molecules were successfully covered by COD. Submicron COD further damaged the cells during the adhesion process, especially for Vero cells in the control group, leading to an elevated amount of attached microcrystals. Conclusion: Submicron COD can further damage injured Vero cells during the adhesion process. The amount of attached microcrystals is proportional to the degree of cell damage. The increased amount of microcrystals that adhered to the injured epithelial cells plays an important role in the formation of early-stage kidney stones.

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Section: Methodsmentioning

confidence: 97%

Interaction between submicron COD crystals and renal epithelial cells

Peng¹,

Ouyang²,

Yao³

et al. 2012

IJN

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“…From a chemical perspective, CaOx stone formation is closely related to: high levels of urine calcium and urine oxalic acid; nucleation, growth, and aggregation of CaOx crystals; and adhesion between crystals and renal tubular epithelial cells. [1][2][3] Therefore, the crystallites in urine are crucial factors affecting the formation of uroliths. [4][5][6] Previous studies 5,6 have found differences between the urinary crystallites (,1000 nm in size) of patients with urolithiasis and healthy subjects without any urolithiasis history.…”

Section: Introductionmentioning

confidence: 99%

Changes in urinary nanocrystallites in calcium oxalate stone formers before and after potassium citrate intake

Duan¹,

Xia²,

Zhang³

et al. 2013

IJN

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Abstract:The property changes of urinary nanocrystallites in 13 patients with calcium oxalate (CaOx) stones were studied before and after ingestion of potassium citrate (K 3 cit), a therapeutic drug for stones. The analytical techniques included nanoparticle size analysis, transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The studied properties included the components, morphologies, zeta potentials, particle size distributions, light intensity autocorrelation curves, and polydispersity indices (PDIs) of the nanocrystallites. The main components of the urinary nanocrystallites before K 3 cit intake included uric acid, β-calcium phosphate, and calcium oxalate monohydrate. After K 3 cit intake, the quantities, species, and percentages of aggregated crystals decreased, whereas the percentages of monosodium urate and calcium oxalate dehydrate increased, and some crystallites became blunt. Moreover, the urinary pH increased from 5.96 ± 0.43 to 6.46 ± 0.50, the crystallite size decreased from 524 ± 320 nm to 354 ± 173 nm, and the zeta potential decreased from −4.85 ± 2.87 mV to −8.77 ± 3.03 mV. The autocorrelation curves became smooth, the decay time decreased from 11.4 ± 3.2 ms to 4.3 ± 1.7 ms, and the PDI decreased from 0.67 ± 0.14 to 0.53 ± 0.19. These changes helped inhibit CaOx calculus formation.

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“…Besides that, in the presence of E.PG and I.PG at concentrations of 1 and 2 g/L, a single peak of absorption between 3474 and 3457 cm − 1 has been founded, affirming the presence the COD crystals. In [77], a bending band out of the water at 780 cm − 1 [78] and also two absorption bands have been observed at 1644 cm − 1 for (vas (COO − )) and 1328 cm − 1 for (vs (COO − )) attesting the formation of COD [74,79].…”

Section: Crystal's Characterization By Fourier Transform Infrared Spementioning

confidence: 93%

Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Kachkoul

Mohim

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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e plant Punica granatum L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. is comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. e quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. e crystallization inhibition has been studied in vitro by the turbidimetric model. e characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. e results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration "IC 50 " obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83 μg/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91 μg/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. ese results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.

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Inhibition on calcium oxalate crystallization and repair on injured renal epithelial cells of degraded soybean polysaccharide

Cited by 25 publications

References 20 publications

Interaction between submicron COD crystals and renal epithelial cells

Interaction between submicron COD crystals and renal epithelial cells

Changes in urinary nanocrystallites in calcium oxalate stone formers before and after potassium citrate intake

Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

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