Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment.

Abstract

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.

Histomorfometri Hepar Ayam Petelur Jantan (Gallus gallus domesticus L.) Setelah Pemberian Tepung Daun Kelor (Moringa Oleifera Lam.) Sebagai Imbuhan Pakan

“…(2021) menyatakan bahwa kandungan flavonoid, tanin dan saponin pada ekstrak tumbuhan Rhizophora apiculata dapat menyebabkan hemoragi, hipertrofi dan nekrosis pada sel hepar. Daun kelor bersifat antibakteri karena memiliki senyawa kimia berupa saponin, tanin, flavonoid, alkaloid, dan antrakuinon (Kenconojati dan Rukman, 2019;Mardiana, 2012). Sifat ini dapat merusak membran sel sehingga dapat menganggu proses transport ion dan mengakibatkan lisis pada sel.…”

HAEMATOLOGY OF SALINE TILAPIA (Oreochromis niloticus) FED WITH MORINGA LEAF POWDER ENRICHED PELLETS

“…These active compounds are flavonoids, tannins, and saponins, thus helping the work of monocytes. Flavonoids are known to have anti-bacterial activity by carrying out mechanisms, namely inhibiting the synthesis of nucleic acids from bacteria, damaging the function of the cytoplasmic membrane, inhibiting the metabolism of bacteria, inhibiting cell membrane synthesis and aggregating bacterial cells 17 .…”

A Mini-Review: Effect of Moringa oleifera Leaves for Fish Health

“…The saponins contained in M. oleifera, although they taste bitter, do not have any harmful effects [71]. However, the lower the amount of antinutrients the better [72].…”

“…Oreochromis niloticus Linn) fry after being given M. oleifera extract at doses of 50, 75 and 100 mg through intra-muscular injection, there was an increase in the number of leukocyt cell. The increase in the amount of leukocyte cell at doses of 75 and 100 mg was quite high, 187.09% and 148.56%, respectively, but the highest survival occurred at a dose of 75 mg (83.33% ± 5.77) with a relative percent survival value (RPS) of72.22% ± 9.62 [63]. The normal range for RPS is> 60% [64].…”