Inhibitive Determination of Hg(II) in Aqueous Solution Using Urease Amperometric Biosensor

Abstract

An amperometric biosensor for the indirect determination of Hg(II) has been developed based on inhibition of urease (EC 3.5.1.5) immobilized into alginate–chitosan polyelectrolyte complexes membrane. The biosensor response was monitored by following the reduction peak of hydrolyzed urea at around -0.15 V. The amperometric biosensor has a dynamic range 40–90 ppb Hg(II) with limit of detection of 66.45 ppb toward Hg(II) ions, repeatability (CV) value of 0.86% and only Ag(I) as the main potential interference. The sensor shows a stable and reproducible response for more than 2 weeks when it stored dry at 4 °C. The analytical results of Hg(II)-spiked water sample showed a good agreement with those obtained by atomic absorption spectrometry method, suggesting that the developed method may be applied in the determination of Hg(II) in the water samples.

Review of microbial biosensor for the detection of mercury in water

“…A potentiometric urease‐based enzymatic biosensor was designed, which was renewable in nature, based on self‐assembled gold nanoparticles for the detection of mercury (Yang et al., 2006). Another amperometric biosensor was designed based on the inhibition of enzymatic urease for the determination of mercury in water samples (Hermanto et al., 2019). Whole‐cell‐based biosensors: A variety of whole‐cell‐based biosensors are used for determining the presence of mercury in environmental and water solutions (Harms et al., 2006; M. Park et al., 2013).…”

“…A potentiometric urease‐based enzymatic biosensor was designed, which was renewable in nature, based on self‐assembled gold nanoparticles for the detection of mercury (Yang et al., 2006). Another amperometric biosensor was designed based on the inhibition of enzymatic urease for the determination of mercury in water samples (Hermanto et al., 2019).…”

Validation of Mercury Speciation Analysis in River Around Artisanal Small-Scale Gold Mining Area in West Nusa Tenggara, Indonesia

“…Mercury in the environment exists in di erent molecular forms according to speci c biogeochemical transformations and ecotoxicity. Inorganic ionic mercury (Hg 2+ ) is a common form/species found in water samples (Hermanto et al, 2019;Hermanto et al, 2022). Particular attention is given to mercury which undergoes a natural transformation called organomercury compounds (Hrubaru et al, 2018) , including the in-situ formation of methylmercury (MeHg) and dimethylmercury (Me 2 Hg).…”

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds

“…At constant substrate concentrations and varying metal ion concentrations, inhibition causes the enzyme catalytic activity to decrease. The reduction in enzyme catalytic activity is proportional to the number of heavy metal ions as the sample's inhibitor (Hermanto, Kuswandi, Siswanta, & Mudasir, 2019).…”